Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (8): 124-129.

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Screening and Identification of Antagonistic Actinomyces 1-g-59 Against Fusarium oxysporium f. sp. cubense

Liu Xiaoyu1, 2 Zhou Dengbo1, 2 Tan Xin2 Gao Zhufen2 Chen Bo1, 2 Huang Xiao1, 2 Zhang Xiyan2   

  1. (1. College of Horticulture and Landscape,Hainan University,Haikou 571737;2. Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences, Haikou 571101)
  • Received:2013-02-25 Revised:2013-08-11 Online:2013-08-11 Published:2013-09-02

Abstract:

Five antagonistic strains against Fusarium oxysporum f. sp. cubense were isolated and screened from the rhizosphere soil of the banana garden in Meitai and Nanbao, strain 1-g-59 possessed the most prominent bioactivity. By studying the morphology, cultural, physiological and biochemical properties, cell wall components analysis, 16S rDNA and phylogenetic tree, the results showed that strain 1-g-59 was identified as Streptomyces lunalinharesii. Pot experiment showed that control effects of the fermentation filtrate of strain 1-g-59 on banana fusarium wilt disease was 64.9%.

Key words: Banana, Fusarium wilt disease, Antagonistic actinomyces, Screening , Identification