Screening of a Chitosanase-producing Strain Cellulophaga sp. and Optimization of the Fermentation Conditions

Abstract

Abstract: A chitosanase-producing bacterium M5 was isolated from Lianyungang sea area. It was identified as Cellulophaga sp. based on its morphological, physiological and biochemical properties and 16S rDNA sequence analysis and named Cellulophaga sp. M5. By single factors combination with orthogonal experimental, the optimum medium composition for producing chitosanase by Cellulophaga sp. M5 was as follows（g/L）：chitosan powder 10, yeast extract 3, ammonium sulfate 5, glucose 1.0, NaCl 5, MgSO₄ ?7H₂O 1.4, K₂HPO₄ ?3H₂O 1.4, the initial pH6.5. The optimum cultivation condition was to incubate in 250 mL flask installed with 70 mL medium of 1% inoculum size at 30℃, 180 r/min for 84 h. The maximal chitosanase activity could reach 6.67 U/mL when Cellulophaga sp. M5 was incubated in flask under the optimum cultural conditions. The activity increased by 1.5 times in comparision with initial activity of 2.67 U/mL.

Key words: Chitosanase Screening, Identification, Fermentation optimization, Cellulophaga

Sun Yuying, Zhang Jiquan, Wu Shiang. Screening of a Chitosanase-producing Strain Cellulophaga sp. and Optimization of the Fermentation Conditions[J]. Biotechnology Bulletin, 2013, 0(8): 150-154.

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