Abstract: Clostridium butyricum, as a probiotic has been studied widely, is a spore-forming, gram-positive anaerobe that is found in intestines of healthy animals and humans. As a novel spore-forming probiotic, C. butyricum can withstand the low pH, heat and many antibiotic compared with non-spore forming probiotics, and exert different health effects in host, such as regulation of intestinal microbial homeostasis, stabilization of the gastrointestinal barrier function, enzymatic activity inducing absorption and nutrition, immunomodulatory effects, improvement of the animal production performance. So it will be used more widely. In this paper, we reviewed advances in the biological function, the fermentation technology and the application in clinic and feed additive of C. butyricum. Some advices were also put forward for further research of C. butyricum.Key words: Clostridium butyricum Probiotic Fermentation Clinic Feed additive