一株红孢囊藻（Rhodosorus sp. SCSIO-45730）总糖和β-葡聚糖的积累特性研究

doi:10.13560/j.cnki.biotech.bull.1985.2020-0639

摘要/Abstract

摘要：

红孢囊藻（Rhodosorus sp.）可以积累高含量多糖,在医药保健和生物能源领域具有潜在开发价值。为进一步评估红孢囊藻（Rhodosorus sp.）产业化潜力,研究了一株红孢囊藻SCSIO-45730在盐度不同的4种培养基中生长及总糖和β-葡聚糖积累特性,结果表明：改良的ASW培养基相对于其他培养基（f/2、Conway和Erdschreiber）更有利于红孢囊藻SCSIO-45730生物量和总糖的积累。以ASW培养基为基础培养基,研究该藻盐度适应性,结果显示：该藻可在18‰-58‰盐度范围内正常生长,随着盐度增加,藻细胞体积逐渐增大且出现聚团现象;在盐度为38‰时,获得最大生物量、总糖含量和产率以及β-葡聚糖产率,分别为13.70 g/L、48.52% DW、412.58 mg/L·d和119.70 mg/L·d,β-葡聚糖含量随着盐度增加而降低;该藻在户外平板光生物反应器中总糖和β-葡聚糖单位体积产量分别为0.73 g/L和0.23 g/L。结果表明,红孢囊藻SCSIO-45730具有较广的盐度适应性、高产总糖和β-葡聚糖潜力,是生产生物乙醇和β-葡聚糖的理想原料。

关键词: 红孢囊藻, 盐度, 总糖, 生物乙醇, β-葡聚糖

Abstract:

Rhodosorus sp. has the potential to be used in medicine,healthcare and bioenergy due to its capacity of accumulating high carbohydrates content. In order to evaluate its industrial potential,the growth,total carbohydrates and β-glucans accumulation of this strain in 4 different culture media with different salinities were investigated. Results showed that the modified ASW medium was more beneficial for the biomass and total carbohydrates accumulation in Rhodosorus sp. SCSIO-45730 compared to other media（f/2,Conway and Erdschreiber）. The salinity adaptability of this alga was studied using ASW medium as the basic medium. Results indicated that this alga grew normally in the salinity of 18‰-58‰. The cell size gradually increased and the algal cell aggregated with the increase of salinity. The highest biomass,total carbohydrates content and productivity,and β-glucans productivity of Rhodosorus sp. SCSIO-45730 were obtained at the salinity of 38‰,which was 13.70 g/L,48.52% DW,412.58 mg/L·d and 119.70 mg/L·d,respectively,while the β-glucans content decreased with the increase of salinity. The total carbohydrates and β-glucans volumetric yield of this alga in outdoor flat-plate photobioreactor were 0.73 g/L and 0.23 g/L,respectively. In conclusion,Rhodosorus sp. SCSIO-45730 is capable of adaptation to a wide range of salinity and has high potential for total carbohydrates and β-glucans production. It is considered as an optimal feedstock for the production of bioethanol and β-glucans.

Key words: Rhodosorus, salinity, total carbohydrates, bioethanol, β-glucans

代禄梅, 李涛, 吴华莲, 吴后波, 向文洲. 一株红孢囊藻（Rhodosorus sp. SCSIO-45730）总糖和β-葡聚糖的积累特性研究[J]. 生物技术通报, 2021, 37(1): 205-214.

DAI Lu-mei, LI Tao, WU Hua-lian, WU Hou-bo, XIANG Wen-zhou. Total Carbohydrates and β-glucans Accumulation of Rhodosorus sp. SCSIO-45730[J]. Biotechnology Bulletin, 2021, 37(1): 205-214.

图/表 8

表1 四种不同培养基成分

	f/2 medium	Conway medium	Erdschreiber medium	ASW medium
NaNO₃/（g·L^-1）	1.50	1.50	1.50	1.50
NaH₂PO₄/（g·L^-1）	0.01	0.03	0.03	/
K₂HPO₄/（g·L^-1）	/	/	/	0.12
NaHCO₃/（g·L^-1）	0.50	/	/	0.04
FeCl₃·6H₂O/（mg·L^-1）	3.16	1.30	1.16	3.15
Na₂EDTA·2H₂O/（mg·L^-1）	4.36	45	/	4.36
MnCl₂·4H₂O/（mg·L^-1）	0.18	0.36	0.50	0.18
ZnCl₂/（mg·L^-1）	/	4.20	0.06	/
ZnSO₄·7H₂O/（mg·L^-1）	0.023	/	/	0.022
CoCl₂·6H₂O/（mg·L^-1）	0.012	4	0.024	0.01
CuSO₄·5H₂O/（mg·L^-1）	0.01	4	/	0.01
（NH₄）₆Mo₇O₂₄·4H₂O/（mg·L^-1）	/	1.80	/	/
Na₂MoO₄·2H₂O/（mg·L^-1）	0.07	/	0.048	0.006
H₃BO₃/（mg·L^-1）	/	33.4	/	/
Thiamin HCl（VB1）/（mg·L^-1）	/	0.20	0.20	/
VB₁₂/（mg·L^-1）	/	0.10	0.10	/
Salinity/‰	25	36	36	28

图1 4种不同培养基对红孢囊藻（Rhodosorus sp. SCSIO-45730）生长的影响

图2 4种不同培养基对红孢囊藻（Rhodosorus sp. SCSIO-45730）总糖和β-葡聚糖积累的影响=== 222A：总糖;B：β-葡聚糖

图3 不同盐度对红孢囊藻（Rhodosorus sp. SCSIO-45730）生长的影响

图4 不同盐度下红孢囊藻（Rhodosorus sp. SCSIO-45730）细胞形态（第8天） A：10‰;B：18‰;C：28‰;D：38%;E：48‰;F：58‰;G：68‰

图5 不同盐度对红孢囊藻（Rhodosorus sp. SCSIO-45730）总糖含量和产率的影响 A：总糖;B：β-葡聚糖

图6 红孢囊藻（Rhodosorus sp. SCSIO-45730）在户外平板光生物反应器中的生长情况 A：生长曲线;B：培养初期藻液;C：培养后期藻液

图7 红孢囊藻（Rhodosorus sp. SCSIO-45730）在户外培养过程中总糖和β-葡聚糖含量的变化

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