生物技术通报 ›› 2021, Vol. 37 ›› Issue (1): 215-222.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0674
收稿日期:
2020-06-02
出版日期:
2021-01-26
发布日期:
2021-01-15
作者简介:
吴敏,女,硕士研究生,研究方向:生物降解;E-mail: 基金资助:
WU Min1(), TANG Jie1(), HU Qiong1, LEI Dan1, ZHANG Qing2
Received:
2020-06-02
Published:
2021-01-26
Online:
2021-01-15
摘要:
旨在探究表面活性剂对菌株降解溴氰菊酯(Deltamethrin,DM)的影响。以前期筛选到具备DM较高降解能力的Acinetobacter junii LH-1-1为目标菌株,通过荧光光谱法测定了3种不同类型表面活性剂(CTAB、AES和Tween-20)的临界胶束浓度(Critical Micelle Concentration,CMC),并探究不同表面活性剂各CMC对A. junii LH-1-1生长、DM增溶作用和菌株降解DM的影响。结果显示,测得CTAB、AES、Tween-20的CMC分别为0.793 mmol/L、0.547 mmol/L和0.031 mmol/L;CTAB对菌株的生长无明显影响,AES对菌株生长有抑制作用,Tween-20对菌株的生长有一定的促进作用;CTAB、AES和Tween-20对DM均具有较强的增溶作用;且CTAB可显著提高A. junii LH-1-1对DM的降解率,当其含量为2 CMC,72 h时DM降解率达到98.20%,较未添加表面活性剂的对照组(阳性)降解率提高了24.31%。阳离子表面活性剂CTAB可显著提高A. junii LH-1-1对DM的降解。
吴敏, 唐洁, 胡琼, 雷丹, 张庆. 表面活性剂对琼式不动杆菌LH-1-1降解溴氰菊酯的影响[J]. 生物技术通报, 2021, 37(1): 215-222.
WU Min, TANG Jie, HU Qiong, LEI Dan, ZHANG Qing. Effects of Surfactants on Deltamethrin Degradation by Acinetobacter junii LH-1-1[J]. Biotechnology Bulletin, 2021, 37(1): 215-222.
[1] |
Wu YQ, Li WH, Yuan MR, et al. The synthetic pyrethroid deltamethrin impairs zebrafish(Danio rerio)swim bladder development[J]. Sci Total Environ, 2020,701:134870.
URL pmid: 31726413 |
[2] | 张小俊, 陆宏达, 田全全, 等. 溴氰菊酯对中华绒螯蟹的毒性作用和组织病理研究[J]. 生态毒理学报, 2018(6):342-351. |
Zhang XJ, Lu HD, Tian QQ, et al. Effects of deltamethrin on histopathology of Chinese Mitten Crab Eriocheir sinensis[J]. Asian Journal of Ecotoxicology, 2018,13(6):342-351. | |
[3] | 洪宇航, 黄毅. 溴氰菊酯对中华绒螯蟹血细胞DNA的损伤[J]. 水产科学, 2018,37(4):544-549. |
Hong YH, Huang Y. Effects of deltamethrin on DNA damage in hemocytes of Chinese Mitten Handed Crab, Eriocheir sinensis[J]. Fisheries Science, 2018,37(4):544-549. | |
[4] | 苏志俊, 刘永涛, 徐春娟, 等. 一株溴氰菊酯降解菌的分离鉴定及降解特性研究[J]. 淡水渔业, 2019,49(1):98-106. |
Su ZJ, Liu YT, Xu CJ, et al. Isolation, identification and degradation characteristics of a deltamethrin-degrading bacterial strain[J]. Freshwater Fisheries, 2019,49(1):98-106. | |
[5] | Zhao JY, Chi YL, et al. Effects of two surfactants and beta-cyclodex-trin on beta-cypermethrin degradation by Bacillus licheniformis B-1[J]. J Agric Food Chem, 2015,50:10729-10735. |
[6] |
Hao XX, Zhang XQ, Duan BH, et al. Screening and genome sequencing of deltamethrin-degrading bacterium ZJ6[J]. Curr Microbiol, 2018,75(11):1468-1476.
URL pmid: 30066155 |
[7] | 孙胜利. 焦化废水中产表面活性剂菌的筛选及其对多环芳烃降解的影响[D]. 广州:华南理工大学, 2018. |
Sun SL. The biosurfactant-producing microorganisms isolated from coking wastewater and its effect on the degradation of polycyclic aromatic hydrocarbons[D]. Guangzhou:South China University of Technology, 2018. | |
[8] | Zhang JS, Sun ZT, Li YY, et al. Biodegradation of p-nitrophenol by Rhodococcus sp. CN6 with high cell surface hydrophobicity[J]. Journal of Hazardous Materials, 2009,163(2-3):723-728. |
[9] |
Zhang C, Wang SH, Yan YC. Isomerization and biodegradation of beta-cypermethrin by Pseudomonas aeruginosa CH7 with biosurfactant production[J]. Bioresource Technology, 2011,102(14):7139-7146.
URL pmid: 21570279 |
[10] | 荣璐阁. 表面活性剂强化微生物修复柴油污染土壤研究[D]. 沈阳:沈阳大学, 2019. |
Rong LG. Study on microbial remediation of diesel contaminated soil by surfactant[D]. Shenyang:Shenyang University, 2019. | |
[11] | 黄慧. 一株产生物表面活性剂的苯酚降解菌的筛选及性能研究[D]. 武汉:武汉科技大学, 2019. |
Huang H. Screening and characterization analysis of a phenol-degrading bacteria of producing biosurfactant[D]. Wuhan:Wuhan University of Science and Technology, 2019. | |
[12] | 郭娟. 考马斯亮蓝G250与CTAB、OP-10的缔合作用研究[D]. 兰州:兰州交通大学, 2018. |
Guo J. The association interactions between CTAB、OP-10 and coomassie brilliant blue G250[D]. Lanzhou:Lanzhou Jiaotong University, 2018. | |
[13] | 柏青海. CTAB及碳纤维负载碘活化PMS降解染料的研究[D]. 杭州:浙江理工大学, 2017. |
Bo QH. Degradation of dyes based on CTAB and lodine supported carbon fibers to activate PMS[D]. Hangzhou:Zhejiang University of Science and Technology, 2017. | |
[14] | 黄敏, 谭丽泉, 余梅, 等. 表面活性剂对白腐真菌降解石油的影响[J]. 石油炼制与化工, 2012,43(8):77-81. |
Huang M, Tan LQ, Yu M, et al. Effect of surfactants on petroleum degradation by white rot fungi[J]. Petroleum Processing and Petrochemicals, 2012,43(8):77-81. | |
[15] | Wei ZS, Hessler CM, George S, et al. Influence of nonionic surfactants on fungal and bacterial degradation of hexane[J]. CLEAN-Soil, Air, Water, 2016,44(7):1-8. |
[16] | 尚琼琼, 张秀霞, 李振伟, 等. 表面活性剂复配强化微生物修复水土体系中的菲[J]. 石油学报(石油加工), 2018,34(3):574-580. |
Shang QQ, Zhang XX, Li ZW, et al. Enhancing microorganism remediation of phenanthrene in water-soil system by mixed surfactants[J]. Acta Petrolei Sinica(Petroleum Processing Section), 2018,34(3):574-580. | |
[17] | 林维佳. 生物表面活性剂对菌株GY2B和F14降解菲的影响机制研究[D]. 广州:华南理工大学, 2017. |
Lin WJ. Mechanism of the effect of biosurfactants on the biodegradation of phenanthrene by bacterial strain GY2B and F14[D]. Guangzhou:South China University of Technology, 2017. | |
[18] | 肖鹏飞, 李玉文, Kondo R. Tween60和SDS强化白腐真菌修复DDT污染土壤[J]. 中国环境科学, 2015,35(12):3737-3743. |
Xiao PF, Li YW, Kondo R. Enhancement of Tween60 and SDS for bioremediation of DDT-contaminated soil by white rot fungus[J]. China Environmental Science, 2015,35(12):3737-3743. | |
[19] | 姜霞, 区自清, 应佩峰, 等. 表面活性剂对分枝杆菌KR2菌株降解菲的影响[J]. 应用生态学报, 2003(11):2011-2014. |
Jiang X, Qu ZQ, Ying PF, et al. Effects of surfactants on degradation of phenanthrene by Mycobacterium sp. KR2[J]. Chinese Journal of Applied Ecology, 2003(11):2011-2014. | |
[20] | Zhong H, Wang ZQ, Liu ZF, et al. Degradation of hexadecane by pseudomonas aeruginosa with the mediation of surfactants:Relation between hexadecane solubilization and bioavailability[J]. International Biodeterioration & Biodegradation, 2016,115:141-145. |
[21] | 王为中, 王向阳, 孙小涵, 等. 表面活性剂液相微萃取在白蚁药物残留分析中的应用研究[J]. 中华卫生杀虫药械, 2017(2):75-78. |
Wang WZ, Wang XY, Sun XH, et al. Application of surfactant-assisted dispersive liquid-phase micro-extraction for detection of termiticide residues[J]. Chinese Journal of Hygienic Insecticides & Equipments, 2017(2):75-78. | |
[22] | Ahmad S, Yasin K. Removal of organic pollutants by using surfactant modified bentonite[J]. Journal of the Chemical Society of Pakistan, 2018,3:447-456. |
[23] |
Cheng M, Zeng GM, Huang DL, et al. Tween 80 surfactant-enhanced bioremediation:toward a solution to the soil contamination by hydrophobic organic compounds[J]. Critical Reviews in Biotechnology, 2018,38(1):1-14.
doi: 10.1080/07388551.2017.1311295 URL pmid: 28462596 |
[24] | 龚铃堰, 廖广志, 陈权生, 等. 表面活性剂溶胀胶束:性能及应用[J]. 物理化学学报, 2019,35(8):816-828. |
Gong LY, Liao GZ, Chen QS, et al. Surfactant swelling micelles:properties and applications[J]. Acta Physico-Chimica Sinica, 2019,35(8):816-828. | |
[25] | 胡琼, 唐洁, 雷丹, 等. 一株溴氰菊酯降解菌的分离鉴定及其降解条件优化[J]. 微生物学通报, 2020,47(3):699-709. |
Hu Q, Tang J, Lei D, et al. Isolation, identification and optimization of degradation conditions of a deltamethrin degrading strain[J]. Microbiology China, 2020,47(3):699-709. | |
[26] | 张春燕, 罗建新, 潘春跃, 等. 光谱法测定十二烷基苯磺酸钠临界胶束浓度[J]. 环境科学与技术, 2016,39(8):99-102. |
Zhang CY, Luo JX, Pang CY, et al. Determination of critical micelle concentration of sodium dodecyl benzene sulfonate by spectrometry[J]. Environ Sci Technol, 2016,39(8):99-102. | |
[27] | 王冠石, 王小永. 添加中低浓度离子液体[bmim][BF4]对吐温-20临界胶束浓度和胶束结构的影响[J]. 化学通报, 2017,80(8):777-782. |
Wang GS, Wang XY. Influence of medium-low concentration of ionic liquid[Bmim][BF4]on critical micellization concentration and micelle structures[J]. Chemistry, 2017,80(8):777-782. | |
[28] | 张栋. 表面活性剂对PAHs微生物界面行为的影响及调控机制[D]. 杭州:浙江大学, 2013. |
Zhang D. Surfactant controlled bacterial interfacial behaviors of PAHs and its mechanisms[D]. Hangzhou:Zhejiang University, 2013. | |
[29] | 鲍根莲, 邓欢欢, 李少君, 等. 生物表面活性剂和化学螯合剂强化无柄小叶榕修复Cd、Cu重金属污染盐碱地[J]. 环境化学, 2019,38(7):1497-1506. |
Bao GL, Deng HH, LI SJ, et al. Biosurfactants generated from marine microbe and chelator assisted remediation of Cadmium and Copper contaminated saline-alkaline soil by Ficus concinna var. Subsessilis[J]. Environ Chem, 2019,38(7):1497-1506. | |
[30] |
Liu S, Guo C, Liang X, et al. Nonionic surfactants induced changes in cell characteristics and phenanthrene degradation ability of Sphingomonas sp. GY2B[J]. Ecotoxicology and Environmental Safety, 2016,129:210-218.
URL pmid: 27045921 |
[31] |
Tehrani-Bagha AR, Holmberg K. Solubilization of hydrophobic dyes in surfactant solutions[J]. Materials, 2013,6(2):580-608.
doi: 10.3390/ma6020580 URL pmid: 28809328 |
[32] |
Lee SY, Kim SJ, Chung SY, et al. Sorption of hydrophobic organic compounds onto organoclays[J]. Chemosphere, 2004,55(5):781-785.
doi: 10.1016/j.chemosphere.2003.11.007 URL pmid: 15013684 |
[33] |
González M, Ma DM, Sánchez L, et al. Pesticide adsorption on a calcareous soil modified with sewage sludge and quaternary alkyl-ammonium cationic surfactants[J]. Environmental Science & Pollution Research, 2008,15(1):8-14.
doi: 10.1065/espr2007.02.387 URL pmid: 18306881 |
[34] |
Lu L, Zhu L. Effect of a cationic surfactant on the volatilization of PAHs from soil[J]. Environmental Science & Pollution Research International, 2012,19(5):1515-1523.
doi: 10.1007/s11356-011-0631-0 URL pmid: 21997282 |
[35] | 闫端, 余晖, 黄国和, 等. 双子表面活性剂CG12-3-12、鼠李糖脂与TX-100对多环芳烃增溶作用的比较研究[J]. 环境科学学报, 2015,35(1):229-237. |
Yan D, Yu H, Huang GH, et al. Effects of gemini surfactant CG12-3-12,rhamnolipid and triton- X-100 on solubility enhancement of PAHs:A comparative study[J]. Acta Scientiae Circumstantiae, 2015,35(1):229-237. | |
[36] | Zhang H, Zhang YM, Hou ZG, et al. Biodegradation potential of deltamethrin by the Bacillus cereus strain Y1 in both culture and contaminated soil[J]. Int Biodeter Biodegradation, 2016,106:53-59. |
[37] | 侯少锋. 溴氰菊酯降解菌剂的制备与应用[D]. 贵阳:贵州大学, 2016. |
Hou SF. Preparation and application of deltamethrin degrading bacteria agent[D]. Guiyang:Guizhou University, 2016. | |
[38] | 杨晓棠. 溴氰菊酯降解菌的筛选及降解特性研究[D]. 长春:吉林农业大学, 2008. |
Yang XT. Study on isolation and degrading characteristics of deltamethrin-degrading bacteria[D]. Changchun:Jilin Agricultural University, 2008. | |
[39] | 向骊羽, 赵浩宇, 黄孟飞, 等. 一株溴氰菊酯降解菌的筛选及其降解特性研究[J]. 四川大学学报:自然科学版, 2012,49(4):914-918. |
Xiang LY, Zhao HY, Huang MF, et al. Isolation and identification of a strain of deltamethrin degrading bacteria and its degradation characteristics[J]. Journal of Sichuan University:Natural Science Edition, 2012,49(4):914-918. |
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