生物技术通报 ›› 2021, Vol. 37 ›› Issue (1): 215-222.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0674

• 研究报告 • 上一篇    下一篇

表面活性剂对琼式不动杆菌LH-1-1降解溴氰菊酯的影响

吴敏1(), 唐洁1(), 胡琼1, 雷丹1, 张庆2   

  1. 1.西华大学食品与生物工程学院,成都 610039
    2.西华大学食品与生物工程学院古法发酵生物技术研究所,成都 610039
  • 收稿日期:2020-06-02 出版日期:2021-01-26 发布日期:2021-01-15
  • 作者简介:吴敏,女,硕士研究生,研究方向:生物降解;E-mail: 1039445831@qq.com
  • 基金资助:
    四川省科技厅自然科学应用基础项目(2019YJ0389);西华大学人才培养重点项目(z1310525)

Effects of Surfactants on Deltamethrin Degradation by Acinetobacter junii LH-1-1

WU Min1(), TANG Jie1(), HU Qiong1, LEI Dan1, ZHANG Qing2   

  1. 1. School of Food and Bioengineering,Xihua University,Chengdu 610039
    2. Institute of Ancient Brewing,School of Food and Bioengineering,Xihua University,Chengdu 610039
  • Received:2020-06-02 Published:2021-01-26 Online:2021-01-15

摘要:

旨在探究表面活性剂对菌株降解溴氰菊酯(Deltamethrin,DM)的影响。以前期筛选到具备DM较高降解能力的Acinetobacter junii LH-1-1为目标菌株,通过荧光光谱法测定了3种不同类型表面活性剂(CTAB、AES和Tween-20)的临界胶束浓度(Critical Micelle Concentration,CMC),并探究不同表面活性剂各CMC对A. junii LH-1-1生长、DM增溶作用和菌株降解DM的影响。结果显示,测得CTAB、AES、Tween-20的CMC分别为0.793 mmol/L、0.547 mmol/L和0.031 mmol/L;CTAB对菌株的生长无明显影响,AES对菌株生长有抑制作用,Tween-20对菌株的生长有一定的促进作用;CTAB、AES和Tween-20对DM均具有较强的增溶作用;且CTAB可显著提高A. junii LH-1-1对DM的降解率,当其含量为2 CMC,72 h时DM降解率达到98.20%,较未添加表面活性剂的对照组(阳性)降解率提高了24.31%。阳离子表面活性剂CTAB可显著提高A. junii LH-1-1对DM的降解。

关键词: 表面活性剂, 琼式不动杆菌LH-1-1, 溴氰菊酯, 生物降解

Abstract:

It is aimed to investigate the effects of surfactants on the degradation of deltamethrin(DM)by strain. The previous screened Acinetobacter junii strain LH-1-1,which has a high degradability on DM,was selected as target strain. The critical micelle concentration(CMC)of three different types of surfactants(CTAB,AES,and Tween-20)werefirst determined by fluorescence spectrometry,and then the effects of the CMCs of CTAB,AES,and Tween-20 on the strain’s growth,and solubility and degradation of DM by the strain were investigated. The CMC of CTAB,AES,and Tween-20 was 0.793 mmol/L,0.547 mmol/L,and 0.031 mmol/L,respectively. The results showed that the strain growth was not significantly affected by using CTAB,while inhibited by AES,and Tween-20 had a certain promotion on the strain’s growth. The solubility of DM was significantly improved by using CTAB,AES and Tween-20. The degradation rate of DM was significantly enhanced by A. junii LH-1-1 in the presence of CTAB. When the concentration of CTAB was 2 CMC,the degradation rate of DM was 98.20% within 72 h,which was 24.31% higher than the positive control group(without the addition of surfactants). The cationic surfactant CTAB may significantly improve the degradation of DM by A. junii LH-1-1.

Key words: surfactant, Acinetobacter junii LH-1-1, deltamethrin, biodegradation