生物技术通报 ›› 2021, Vol. 37 ›› Issue (1): 205-214.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0639

• 研究报告 • 上一篇    下一篇

一株红孢囊藻(Rhodosorus sp. SCSIO-45730)总糖和β-葡聚糖的积累特性研究

代禄梅1,2(), 李涛1,3,4, 吴华莲1,3,4, 吴后波1,3,4, 向文洲1,3,4()   

  1. 1.中国科学院南海海洋研究所 中国科学院热带海洋生物资源与生态重点实验室,广州 510301
    2.中国科学院大学,北京 100049
    3.南方海洋科学与工程广东省实验室(广州),广州 511458
    4.中国科学院南海生态环境工程创新研究院,广州 510301
  • 收稿日期:2020-05-25 出版日期:2021-01-26 发布日期:2021-01-15
  • 作者简介:代禄梅,女,博士研究生,研究方向:微藻生物技术;E-mail: dlmei610@163.com
  • 基金资助:
    广东省省级科技计划项目(2019B030316027);北海市“十三五”海洋经济创新发展示范项目(Bhsfs004);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0406);中国科学院重点部署项目(ZDRW-ZS-2017-2-2);广东省新能源和可再生能源研究开发与应用开放基金(E039kf0301)

Total Carbohydrates and β-glucans Accumulation of Rhodosorus sp. SCSIO-45730

DAI Lu-mei1,2(), LI Tao1,3,4, WU Hua-lian1,3,4, WU Hou-bo1,3,4, XIANG Wen-zhou1,3,4()   

  1. 1. Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301
    2. University of Chinese Academy of Sciences,Beijing 100049
    3. Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou 511458
    4. Institute of South China Sea Ecology and Environmental Engineering,Chinese Academy of Sciences,Guangzhou 510301
  • Received:2020-05-25 Published:2021-01-26 Online:2021-01-15

摘要:

红孢囊藻(Rhodosorus sp.)可以积累高含量多糖,在医药保健和生物能源领域具有潜在开发价值。为进一步评估红孢囊藻(Rhodosorus sp.)产业化潜力,研究了一株红孢囊藻SCSIO-45730在盐度不同的4种培养基中生长及总糖和β-葡聚糖积累特性,结果表明:改良的ASW培养基相对于其他培养基(f/2、Conway和Erdschreiber)更有利于红孢囊藻SCSIO-45730生物量和总糖的积累。以ASW培养基为基础培养基,研究该藻盐度适应性,结果显示:该藻可在18‰-58‰盐度范围内正常生长,随着盐度增加,藻细胞体积逐渐增大且出现聚团现象;在盐度为38‰时,获得最大生物量、总糖含量和产率以及β-葡聚糖产率,分别为13.70 g/L、48.52% DW、412.58 mg/L·d和119.70 mg/L·d,β-葡聚糖含量随着盐度增加而降低;该藻在户外平板光生物反应器中总糖和β-葡聚糖单位体积产量分别为0.73 g/L和0.23 g/L。结果表明,红孢囊藻SCSIO-45730具有较广的盐度适应性、高产总糖和β-葡聚糖潜力,是生产生物乙醇和β-葡聚糖的理想原料。

关键词: 红孢囊藻, 盐度, 总糖, 生物乙醇, β-葡聚糖

Abstract:

Rhodosorus sp. has the potential to be used in medicine,healthcare and bioenergy due to its capacity of accumulating high carbohydrates content. In order to evaluate its industrial potential,the growth,total carbohydrates and β-glucans accumulation of this strain in 4 different culture media with different salinities were investigated. Results showed that the modified ASW medium was more beneficial for the biomass and total carbohydrates accumulation in Rhodosorus sp. SCSIO-45730 compared to other media(f/2,Conway and Erdschreiber). The salinity adaptability of this alga was studied using ASW medium as the basic medium. Results indicated that this alga grew normally in the salinity of 18‰-58‰. The cell size gradually increased and the algal cell aggregated with the increase of salinity. The highest biomass,total carbohydrates content and productivity,and β-glucans productivity of Rhodosorus sp. SCSIO-45730 were obtained at the salinity of 38‰,which was 13.70 g/L,48.52% DW,412.58 mg/L·d and 119.70 mg/L·d,respectively,while the β-glucans content decreased with the increase of salinity. The total carbohydrates and β-glucans volumetric yield of this alga in outdoor flat-plate photobioreactor were 0.73 g/L and 0.23 g/L,respectively. In conclusion,Rhodosorus sp. SCSIO-45730 is capable of adaptation to a wide range of salinity and has high potential for total carbohydrates and β-glucans production. It is considered as an optimal feedstock for the production of bioethanol and β-glucans.

Key words: Rhodosorus, salinity, total carbohydrates, bioethanol, β-glucans