磁性纳米材料载体固定纤维素酶技术研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2015.08.009

摘要/Abstract

摘要： 生物质原料转化为还原性糖,从而为生物质燃料乙醇的生产提供基础原料。针对现在游离酶生产工艺中的不足,研究者提出了纤维素酶固定化技术,其中以磁性纳米材料作为纤维素酶的固定化载体,不仅可提高纤维素酶的催化性能,增强酶的稳定性,而且以外加磁场代替传统的机械搅拌方式可充分发挥载体材料的磁响应性,从而使制备的固定化酶从反应体系中易于分离,高效且具有重复性。研究者们提出了很多优秀的纤维素酶固定化方案,综述了近年来磁性纳米材料固定纤维素酶的不同方法,并对其做了较为详细的阐述,在此基础上进一步对其优缺点和发展前景进行了讨论。

关键词: 生物乙醇, 纤维素酶, 酶固定化, 磁性纳米载体

Abstract: Conversion of biomass to reducing sugar by cellulase is the foundation of the biomass fuel production. Nowadays researchers have invented the enzyme immobilization technology to avoid the disadvantages of free enzymatic hydrolization. Utilization of magnetic nanoparticles as carriers for the cellulase immobilization can increase the catalytic activities of enzyme and enhance stability of the enzyme. Moreover, replacing conventional mechanical stirring by external magnetic field can give full play to the role of carriers’ magnetic response; with the immobilization on magnetic nanoparticles, the produced cellulase can be separated easily from the mixture in the reaction systems. Researchers have proposed many excellent immobilization methods. Here the different methods of cellulase immobilization by magnetic nanoparticles are reviewed, their applications are explained in detail, and consequently the advantages and disadvantages as well as the development prospect are discussed.

Key words: bioethanol, cellulase, cellulase immobilization, magnetic nanoparticles as carrier

邢朝晖, 苏跃龙, 张琦, 阮馨怡, 林燕, 王欣泽, 孔海南. 磁性纳米材料载体固定纤维素酶技术研究进展[J]. 生物技术通报, 2015, 31(8): 59-65.

Xing Zhaohui, Su Yuelong, Zhang Qi, Ruan Xinyi, Lin Yan, Wang Xinze, Kong Hainan. Research Progress on Cellulase Immobilized by Magnetic Nanoparticles as Carriers[J]. Biotechnology Bulletin, 2015, 31(8): 59-65.

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