不同地区药用植物两面针根际土壤真菌种群多样性差异分析

doi:10.13560/j.cnki.biotech.bull.1985.2020-0667

摘要/Abstract

摘要： 为了解不同地区两面针植物根际土壤真菌种群多样性差异,以6个不同地区两面针植物根际土壤为研究对象,对土壤真菌ITS1-2进行高通量测序,通过对比分析两面针植物根际土壤真菌的种群多样性差异,并结合冗余分析探讨了环境因素对真菌菌群的影响。研究表明,6个土壤样品真菌种群多样性丰富,但种群结构和物种丰富存在明显差异。子囊菌门（Ascomycota）、未分类菌门（unclassified）、担子菌门（Basidiomycota）、被孢霉门（Mortierellomycota）和罗兹菌门（Rozellomycota）为共有菌门。各样品的优势菌群构成不尽相同,但子囊菌门（Ascomycota）的优势比较明显。真菌种群多样性由高到低的顺序为B3>A3>A2>B2>B1>A1。土壤酸碱度（PH）、有机质含量（ORM）、土壤粘粒（CP）、土壤砂粒（SP）、土壤粉（砂）粒（SSG）等环境因子均对样品的真菌菌群组成和物种丰度有影响,但对各样品的影响程度有差异。

关键词: 两面针, 根际土壤真菌, 高通量测序, 种群多样性

Abstract: To understand the community diversity differences of fungi in the rhizosphere soil of Zanthoxylum nitidum（Roxb.）DC in different regions,the rhizosphere soils of Z. nitidum in 6 different regions were studied. The ITS1-2 of fungi in soil samples was sequenced on the high-throughput platform. The community diversity differences of fungi in the rhizosphere soil were analyzed by contrast,and the effect of environmental factors on fungal community was discussed combined redundancy analysis. Research showed that the community diversity of fungi in 6 regions was rich,while there were significant differences in fungal community structure and species richness among the samples. Ascomycota,unclassified,Basidiomycota,Mortierellomycota and Rozelomycota were common. The dominant community composition of each sample varied,but the dominance of Ascomycete was significant. The order of fungal community diversity from high to low was as B3> A3> A2> B2> B1> A1. Environmental factors such as soil pH,organic matter content,soil clay,soil sand,and soil powder（sand）grains all can affect the community composition and species abundance of fungi in the samples,but the influence degree of environmental factors on each sample differed.

Key words: Zanthoxylum nitidum（Roxb.）DC, fungi in the rhizosphere soil, high-throughput sequencing, community diversity

张淼, 陈裕凤, 陈龙, 黄飘玲, 韦露玲. 不同地区药用植物两面针根际土壤真菌种群多样性差异分析[J]. 生物技术通报, 2020, 36(9): 167-179.

ZHANG Miao, CHEN Yu-feng, CHEN Long, HUANG Piao-ling, WEI Lu-ling. Difference Analysis of the Community Diversity of Fungi in the Rhizosphere Soil of Zanthoxylum nitidum（Roxb.）DC in Different Regions[J]. Biotechnology Bulletin, 2020, 36(9): 167-179.

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