苦参根际高效固氮菌的分离及复合菌肥对幼苗的促生效应

doi:10.13560/j.cnki.biotech.bull.1985.2020-0379

生物技术通报 ›› 2020, Vol. 36 ›› Issue (9): 157-166.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0379

• 根际微生物专题(专题主编：张瑞福研究员) • 上一篇下一篇

苦参根际高效固氮菌的分离及复合菌肥对幼苗的促生效应

雷海英¹, 赵青松¹, 杨潇¹, 王毛毛¹, 白洁¹, 孙永琪¹, 王志军²

1.长治学院生物科学与技术系长治 046011;
2.长治学院化学系长治 046011

收稿日期:2020-04-07 出版日期:2020-09-26 发布日期:2020-09-30
作者简介:雷海英,女,硕士研究生,研究方向：植物学;E-mail：xiaoleikkxx@163.com
基金资助:
国家自然科学基金项目（21201024）,山西省应用基础研究项目（201801D221323）,山西省高校科技创新资助项目（201802107）,山西省“1331工程”重点创新研究团队项目（2018018）,山西省高等学校大学生创新创业训练项目（2020606）

Isolation of Efficient Nitrogen-fixing Bacteria from the Rhizosphere of Sophora flavescens and the Growth-promoting Effect of Compound Microbial Fertilizer on Seedlings

LEI Hai-ying¹, ZHAO Qing-song¹, YANG Xiao¹, WANG Mao-mao¹, BAI Jie¹, SUN Yong-qi¹, WANG Zhi-jun²

1. Faculty of Biology Sciences and Technology,Changzhi University,Changzhi 046011;
2. Department of Chemistry,Changzhi University,Changzhi 046011

Received:2020-04-07 Published:2020-09-26 Online:2020-09-30

摘要/Abstract

摘要： 为获得苦参根际促生菌（Plant growth promoting rhizobacteria,PGPR）并明确其促生特性,以促进复合菌肥在苦参育苗基质中的应用。采用平板涂布法从三年生苦参根际土壤中分离根际高效固氮细菌,通过形态特征、生理生化特性及16S rRNA 基因序列进行鉴定,测定其产IAA、溶磷等促生能力及固氮酶活性,筛选出优良菌株进行盆栽苦参苗的促生作用研究。结果分离出了20株苦参根际PGPR 菌,经鉴定分别属于假单胞菌属（Pseudomonas）、芽孢杆菌属（Bacillus）、剑菌属（Ensifer）、节杆菌属（Arthrobacter）以及表皮葡萄球菌属（Staphylococcus）;其中有10株为假单胞菌属和4株芽孢杆菌属菌株;其中芽孢杆菌属菌株NF2-4产IAA能力及溶磷能力（分别为451 mg/L和1.908 mg/L）的综合能力较强,其固氮酶活性为1.24 nmol/（g·h）,选取NF2-4和促生能力较强的假单胞菌属NF1-17作为复合菌肥进行盆栽试验,结果表明：对苦参幼苗的促生长能力大小为复合菌剂 > 菌剂NF2-4 > 菌剂NF1-17 > 对照组。复合发酵菌剂对苦参幼苗表现出的最好的促生效应。

关键词: 土壤, 生物固氮, 植物促生菌, 复合微生物菌肥

Abstract: This work aims to obtain plant growth promoting rhizobacteria（PGPR）and clarify its growth-promoting characteristics,so as to promote the application of compound microbial fertilizer in matrix for Sophora flavescens seedlings. Serial dilution-plating method was used to isolate highly efficient rhizosphere nitrogen-fixing bacteria from the rhizosphere soil of three-year-old Sophora flavescens. The isolated bacteria were identified by morphological characteristics,physiological and biochemical characteristics and 16S rRNA gene sequence. Their growth-promoting ability of IAA production and phosphate-solubilizing,and nitrogenase activity were determined for screening fine strains to study their growth-promoting effects on potted S. flavescens seedlings. As a result,20 strains of S. flavescens PGPR were isolated and identified as Pseudomonas,Bacillus,Ensifer,Arthrobacter and Staphylococcus epidermidis;among them,10 strains were Pseudomonas and 4 strains were Bacillus. Bacillus strain NF2-4 had the strongest IAA production of 451 mg/L and phosphate-solubilizing capacity of 1.908 mg/L,the nitrogenase activity was 1.24 nmol/（g·h）,and thus NF2-4 and Pseudomonas sp. NF1-17 with the stronger growth promotion were used as the compound microbial fertilizer for pot experiment. The results showed that the growth promotion ability to S. flavescens seedlings was compound microbial fertilizer > microbial fertilizer NF2-4 > microbial fertilizer NF1-17 > control group. The compound microbial fertilizer demonstrated the best growth promoting effect on S. flavescens seedling.

Key words: soil, biological nitrogen fixation, plant growth-promoting rhizobacteria, compound microbial fertilizer

雷海英, 赵青松, 杨潇, 王毛毛, 白洁, 孙永琪, 王志军. 苦参根际高效固氮菌的分离及复合菌肥对幼苗的促生效应[J]. 生物技术通报, 2020, 36(9): 157-166.

LEI Hai-ying, ZHAO Qing-song, YANG Xiao, WANG Mao-mao, BAI Jie, SUN Yong-qi, WANG Zhi-jun. Isolation of Efficient Nitrogen-fixing Bacteria from the Rhizosphere of Sophora flavescens and the Growth-promoting Effect of Compound Microbial Fertilizer on Seedlings[J]. Biotechnology Bulletin, 2020, 36(9): 157-166.

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苦参根际高效固氮菌的分离及复合菌肥对幼苗的促生效应

Isolation of Efficient Nitrogen-fixing Bacteria from the Rhizosphere of Sophora flavescens and the Growth-promoting Effect of Compound Microbial Fertilizer on Seedlings

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