外生菌根真菌彩色豆马勃（Pisolithus tinctorius）辅助植物修复重金属Cu污染土壤的应用潜力

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.019

摘要/Abstract

摘要： 旨在研究Pisolithus tinctorius（Pt）菌种的耐铜性以及对宿主植物黑松（Pinus thunbergii）幼苗生长发育、营养元素以及重金属Cu吸收的影响,探究其修复重金属污染土壤的应用潜力。采用液体纯培养,0、5、50、100、250和500 µmol/L浓度Cu处理研究Pt菌种耐Cu性;幼苗盆栽实验（Pt菌根或未菌根化）,分析幼苗生长、各部分营养元素及Cu含量。结果显示,Pt菌种具有很强的耐Cu性,其半抑制浓度达200 µmol/L;与非接菌相比,Pt菌种的侵染可以显著（F=44.57,P=0.003）促进黑松幼苗的生长,提高宿主幼苗根及茎中P和Ca的含量,缓解Cu毒害;接种后幼苗地上部重金属浓度虽有所降低,但可通过增加宿主生物量来提高植物对土壤中重金属的提取效率;接种Pt,可降低土壤中可交换态Cu含量,降低Cu对植物的毒害。耐Cu性的 Pt菌种可以促进宿主幼苗的生长及营养元素的吸收,减少Cu毒害,提高黑松对Cu的提取效率,在Cu污染地的生物修复、植被恢复中具有一定的应用潜力。

关键词: 彩色豆马勃, 黑松, Cu耐性, 提取, 植被恢复

Abstract: The purposes of this paper are to study copper tolerance of Pisolithus tinctorius（Pt）and the effects of Pt on host pine（Pinus thunbergii）seedlings growth,nutrients and heavy metal（Cu）absorption,and to probe its potential for mitigating Cu-contaminated soil. Pt was treated by Cu at concentrations of 0（control）,5,50,100,250,and 500 µmol/L in pure culture with the pot experiment. The growth,nutrients,and Cu concentrations of different parts of seedlings（inoculated with or without Pt）were analyzed. Results showed that Pt strain presented greater Cu tolerance,and its 50% tolerance index（TI）was about 200 µmol/L. Inoculation with Pt significantly improved the growth of host plant（F = 44.57,P = 0.003）and the contents of nutrients such as P and Ca in roots and stems compared with non-inoculated ones,thus mitigating the toxic damage of Cu to host pine. The extraction efficiency of heavy metals in the soil could be improved by increasing biomass of host plants,though Cu concentration in the shoots of inoculated seedlings was decreased. Besides,inoculation with Pt reduced the toxicity of Cu to host plants via reducing the soil exchangeable Cu content. Conclusively,Cu-tolerant fungus Pt may improve the growth and nutrient absorption of host seedlings,as well as reduce the toxicity of Cu to host,improve the extraction efficiency of P. thunbergii to Cu. It shows application potential in phytoremediation and vegetation restoration in Cu-contaminated soil.

Key words: Pisolithus tinctorius（Pt）, Pinus thunbergii, copper tolerance, extraction, vegetation restoration

温祝桂, 王杰, 汤阳泽, 史良, 洪立洲, 沈振国, 陈亚华. 外生菌根真菌彩色豆马勃（Pisolithus tinctorius）辅助植物修复重金属Cu污染土壤的应用潜力[J]. 生物技术通报, 2017, 33(4): 149-156.

WEN Zhu-gui, WANG Jie, TANG Yang-ze, SHI Liang, HONG Li-zhou, SHEN Zhen-guo, CHEN Ya-hua. The Application Potential of Ectomycorrhizal Fungus Pisolithus tinctorius Assisting Plant in Phytoremediation of Cu-contaminated Soils[J]. Biotechnology Bulletin, 2017, 33(4): 149-156.

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