从功能部件看小干扰RNA表达载体的发展

doi:10.13560/j.cnki.biotech.bull.1985.2015.04.009

生物技术通报 ›› 2015, Vol. 31 ›› Issue (3): 64-69.doi: 10.13560/j.cnki.biotech.bull.1985.2015.04.009

从功能部件看小干扰RNA表达载体的发展

侯莹¹, 孙西魁¹, 唐明青^1，2,

(1.华侨大学生物医学学院&分子药物研究院，泉州 362021；2.分子药物教育部工程研究中心，厦门 361021)

收稿日期:2014-09-02 出版日期:2015-03-16 发布日期:2015-03-16
作者简介:侯莹，女，硕士研究生；研究方向：非编码RNA；E-mail：hy552014@163.com
基金资助:
国家“863”计划项目(2012AA020810)，国家自然科学基金项目(81271692)，华侨大学“中央高校基本科研业务费”项目(JB-JC1004)，华侨大学人才引进项目(14BS111)

Evaluating the Development of Small Interfering RNA Expression Vector from Its Functional Elements

Hou Ying¹ Sun Xikui¹ Tang Mingqing ^1，2

(1. School of Biomedical Sciences and Institute of Molecular Medicine，Huaqiao University，Quanzhou 362021；2. Engineering Research Center of Molecular Medicine，Ministry of Education，Xiamen 361021)

Received:2014-09-02 Published:2015-03-16 Online:2015-03-16

摘要/Abstract

摘要： RNA干扰作为转录后基因沉默的有效途径，在基因调控、功能分析及疾病防治等领域发挥重要作用。小干扰RNA表达载体的诞生实现了RNA干扰技术持续、稳定和可控性应用，是实现基因沉默的理想选择。目前干扰性小RNA表达载体虽已发展到第二代，也开发出多种商品化的产品，但依然未能很好地解决其高效、安全、可控性方面的矛盾，发展陷入了瓶颈期。因此，从载体自身角度出发，通过系统分析其功能部件的特点，纵观小RNA载体的历史渊源、发展现状、存在问题和发展方向等问题，为干扰性小RNA表达载体的优化与选择提供相关理论指导。

关键词: 小干扰RNA, 表达载体, 表达元件, 短发夹式RNA载体, 人工微小RNA载体

Abstract: RNA interference as a potent gene silencing approach plays important parts in gene regulation, functional study and gene therapy. The small interfering RNA expression vector achieves a sustainable, stable and controllable application of the RNAi technology, and becomes a promising way for gene silencing. Although the interfering RNA expression vector has progressed to the second generation, and many commercial products were availabe, the discrepancies among efficiency, safety and controllability of these vectors still exist. The development of the interfering RNA expression vector seems get into a lag phase. Therefore, this article analysed the history and development of these small interference RNA expression vectors on the basis of their functional blocks, providing a theoretical fundation for the vector’s optimization and selection.

Key words: small interference RNA, expression vector, expression component, short haipin RNA vector, artificial microRNA vector

侯莹, 孙西魁, 唐明青. 从功能部件看小干扰RNA表达载体的发展[J]. 生物技术通报, 2015, 31(3): 64-69.

Hou Ying, Sun Xikui, Tang Mingqing. Evaluating the Development of Small Interfering RNA Expression Vector from Its Functional Elements[J]. Biotechnology Bulletin, 2015, 31(3): 64-69.

参考文献

[1] Wheeler LA, Vrbanac V, Trifonova R, et al. Durable knockdown and protection from HIV transmission in humanized mice treated with gel-formulated CD4 aptamer-siRNA chimeras[J]. Mol Ther, 2013, 21(7)：1378-1389.
[2] Li Y, Lu J, Han Y, et al. RNA interference functions as an antiviral immunity mechanism in mammals[J]. Science, 2013, 342(6155)：231-234.
[3] Dow LE, Premsrirut PK, Zuber J, et al. A pipeline for the generation of shRNA transgenic mice[J]. Nat Protoc, 2012, 7(2)：374-393.
[4] Wu CS, Yen CJ, Chou RH, et al. Downregulation of microRNA-15b by hepatitis B virus X enhances hepatocellular carcinoma prolifera-tion via fucosyltransferase 2-induced Globo H expression[J]. Int J Cancer, 2014, 134(7)：1638-1647.
[5] Brummelkamp TR, Bernards R, Agami R. A system for stable expression of short interfering RNAs in mammalian cells[J]. Science, 2002, 296(5567)：550-553.
[6] Moffat J, Grueneberg DA, Yang X, et al. A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen[J]. Cell, 2006, 124(6)：1283-1298.
[7] Yan F, Lu Y, Wu G, et al. A simplified method for constructing artificial microRNAs based on the osa-MIR528 precursor[J]. J Biotechnol, 2012, 160(3)：146-150.
[8] Diederichs S, Jung S, Rothenberg SM, et al. Coexpression of Argonaute-2 enhances RNA interference toward perfect match binding sites[J]. Proc Natl Acad Sci USA, 2008, 105(27)：9284-9289.
[9] Liang G, Chen S, Zhu Y, et al. Construction of miRNA eukaryotic expression vector and its stable expression in human liver cancer cells[J]. Procedia Environ Sci, 2011, 8：451-456.
[10] Wu P, Wilmarth MA, Zhang F, et al. miRNA and shRNA expression vectors based on mRNA and miRNA processing[J]. Methods Mol Biol, 2013, 936：195-207.
[11] Schopman NC, Liu YP, Konstantinova P, et al. Optimization of shRNA inhibitors by variation of the terminal loop sequence[J]. Antiviral Res, 2010, 86(2)：204-211.
[12] Wang CM, Wang Y, Fan CG, et al. miR-29c targets TNFAIP3, inhibits cell proliferation and induces apoptosis in hepatitis B virus-related hepatocellular carcinoma[J]. Biochem Biophys Res Commun, 2011, 411(3)：586-592.
[13] Yang L, Li Q, Wang Q, et al. Silencing of miRNA-218 promotes migration and invasion of breast cancer via Slit2-Robo1 pathway[J]. Biomed Pharmacother, 2012, 66(7)：535-540.
[14] Baek MN, Jung KH, Halder D, et al. Artificial microRNA-based neurokinin-1 receptor gene silencing reduces alcohol consumption in mice[J]. Neurosci Lett, 2010, 475(3)：124-128.
[15] Hu T, Fu Q, Chen P, et al. Construction of an artificial MicroRNA expression vector for simultaneous inhibition of multiple genes in mammalian cells[J]. Int J Mol Sci, 2009, 10(5)：2158-2168.
[16] Wu J, Bonsra AN, Du G. pSM155 and pSM30 vectors for miRNA and shRNA expression[J]. Methods Mol Biol, 2009, 487(2)：205-219.
[17] Zeng Y, Wagner EJ, Cullen BR. Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells[J]. Mol Cell, 2002, 9(6)：1327-1333.
[18] Chung KH, Hart CC, Al-Bassam S, et al. Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155[J]. Nucleic Acids Res, 2006, 34(7)：e53.
[19] Yue J, Sheng Y, Ren A, et al. A miR-21 hairpin structure-based gene knockdown vector[J]. Biochem Biophys Res Commun, 2010, 394(3)：667-672.
[20] Boudreau RL, Monteys AM, Davidson BL. Minimizing variables among hairpin-based RNAi vectors reveals the potency of shRNAs[J]. RNA, 2008, 14(9)：1834-1844.
[21] Kozomara A, Griffiths-Jones S. miRBase：integrating microRNA annotation and deep-sequencing data[J]. Nucleic Acids Res, 2011, 39(suppl 1)：D152-D157.
[22] Shibata A, Iwaki A, Fukumaki Y. A novel expression system for artificial miRNAs containing no endogenous miRNA precursor sequences[J]. J RNAi Gene Silencing, 2007, 3(1)：237-237.
[23] Schopman NC, Liu YP, Konstantinova P, et al. Optimization of shRNA inhibitors by variation of the terminal loop sequence[J]. Antiviral Res, 2010, 86(2)：204-211.
[24] Liu C, Zhang L, Sun J, et al. The artificial microRNA mediates GUS-GFP gene silencing using ath-miR169d precursor as backbone[J]. Life Sci J, 2009, 6(2)：1-7.
[25] van Gestel MA, van Erp S, Sanders LE, et al. shRNA-induced saturation of the microRNA pathway in the rat brain[J]. Gene Ther, 2014, 21(2)：205-211.
[26] Chumakov SP, Kravchenko JE, Prassolov VS, et al. Efficient downregulation of multiple mRNA targets with a single shRNA-expressing lentiviral vector[J]. Plasmid, 2010, 63(3)：143-149.
[27] Cifuentes D, Xue H, Taylor DW, et al. A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity[J]. Science, 2010, 328(5986)：1694-1698.
[28] Carrasquillo R, Tian D, Krishna S, et al. SNF8, a member of the ESCRT-II complex, interacts with TRPC6 and enhances its channel activity[J]. BMC Cell Biology, 2012, 13(1)：33-44.
[29] Pu C, Wang L, Miao X, et al. Optimized Tandem amLRNA Mediates Stronger Inhibitory Effects on Hepatitis B Virus Infection[J]. J Gastrointestin Liver Dis, 2011, 20(3)：271-278.
[30] Yan H, Deng X, Cao Y, et al. A novel approach for the construction of plant amiRNA expression vectors[J]. J Biotechnol, 2011, 151(1)：9-14.
[31] Fellmann C, Hoffmann T, Sridhar V, et al. An optimized microRNA backbone for effective single-copy RNAi[J]. Cell Rep, 2013, 5(6)：1704-1713.
[32] Chen SCY, Stern P, Guo Z, et al. Expression of multiple artificial microRNAs from a chicken miRNA126-based lentiviral vector[J]. PLoS One, 2011, 6(7)：e22437.
[33] Bhagwat B, Chi M, Su L, et al. An in vivo transient expression system can be applied for rapid and effective selection of artificial microRNA constructs for plant stable genetic transformation[J]. J Genet Genomics, 2013, 40(5)：261-270.

从功能部件看小干扰RNA表达载体的发展

Evaluating the Development of Small Interfering RNA Expression Vector from Its Functional Elements

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	吴坤坤, 徐行, 季策, 任建峰, 李伟明, 张庆华. 斑马鱼notch3基因真核表达载体的构建及其表达分析[J]. 生物技术通报, 2022, 38(1): 179-186.
[2]	巩元勇, 赵丽华, 闫飞, 郭书巧, 束红梅, 倪万潮. 琉璃苣BoD6D载体的构建及转化[J]. 生物技术通报, 2021, 37(3): 227-232.
[3]	胡晓, 王宝宝, 窦少华, 姜南, 付常振, 金行, 高凤山. 烟台黑猪SLA-2基因真核表达载体的构建及表达[J]. 生物技术通报, 2021, 37(10): 143-151.
[4]	孟利, 杜彩萍. 大鼠His-Akt1重组蛋白的真核表达、蛋白纯化及活性鉴定[J]. 生物技术通报, 2020, 36(12): 98-103.
[5]	陈和锋, 朱晁谊, 李爽. 产瓦伦西亚烯酿酒酵母的表达载体适配及发酵碳氮源优化[J]. 生物技术通报, 2020, 36(1): 209-219.
[6]	翟晓鑫, 高花, 姜平, 许崇波, 张宗辉, 李文哲, 高凤山. 荷包猪SLA-2-HB01真核表达载体的构建及在PK15细胞中的表达[J]. 生物技术通报, 2018, 34(12): 132-139.
[7]	赵佳福, 许厚强, 宋书弦, 段志强, 陈祥. BLM解旋酶基因的克隆、表达载体构建及表达研究[J]. 生物技术通报, 2018, 34(11): 152-159.
[8]	邱艳红,王超楠,朱水芳. 黄瓜花叶病毒致病性研究进展[J]. 生物技术通报, 2017, 33(9): 10-16.
[9]	赵忠娟,魏艳丽,李纪顺,王贻莲,杨合同. 一种根癌农杆菌介导的耐盐椒样薄荷含芽茎段转化系统[J]. 生物技术通报, 2017, 33(7): 126-132.
[10]	肖自华,高飞,孙会改,周宜君. 蒙古沙冬青AmMYB4-like基因的克隆与表达分析[J]. 生物技术通报, 2017, 33(5): 108-116.
[11]	张晶晶, 金小宝, 李小波, 汪洁, 马艳. 过表达Glypican-3对高转移性肝癌细胞HCCLM3生物学行为的影响[J]. 生物技术通报, 2017, 33(12): 176-184.
[12]	王法微, 董金晔, 李晓薇, 刘洋, 吴学彦, 王南, 董园园, 陈欢, 李海燕. 山葡萄CBF基因表达载体构建及对拟南芥根生长的影响[J]. 生物技术通报, 2016, 32(1): 109-114.
[13]	束红梅, 郭书巧, 巩元勇, 倪万潮. 油菜素内酯基因BAS1根系表达载体的构建及烟草的遗传转化[J]. 生物技术通报, 2015, 31(6): 106-110.
[14]	孙璐, 刘志文, 邹丹, 李丹, 潘博, 丛丽娜. 海参溶菌酶枯草芽孢杆菌基因工程菌构建[J]. 生物技术通报, 2014, 0(6): 150-154.
[15]	江伟华, 刘益丽, 江明锋. 大肠杆菌外源蛋白表达载体稳定性的研究进展[J]. 生物技术通报, 2014, 0(5): 25-31.