甲壳动物RNAi技术研究与应用进展

doi:10.13560/j.cnki.biotech.bull.1985.2015.04.008

摘要/Abstract

摘要： RNA干扰(RNA interference，RNAi)是指由双链RNA(double-stranded RNA，dsRNA)诱发的一种使特定基因沉默的现象。作为一种研究基因功能、发现新基因和抗病毒的新型手段，近年来RNAi技术在昆虫、真菌、植物和哺乳动物等的研究中已被广泛应用，但是在甲壳动物研究中尚处于起步阶段。对甲壳动物RNAi技术实施方法做了简要介绍；着重综述了RNAi技术在甲壳动物CHH家族神经肽类基因功能、蜕皮和生长调控机制、配子及性腺发育调控和甲壳动物抗病毒机制研究上的应用进展。

关键词: RNAi, 甲壳动物, 基因功能, 抗病毒

Abstract: RNA interference(RNAi)is a phenomenon that the gene is post-transcriptionally inhibited by using specific double-stranded RNA(dsRNA), gene silencing will be happened eventually. As a new technology, RNAi contributes to the understanding of the the functions and mechanisms of genes. RNAi is widely used in insects, fungus, plants and mammals, however, the reseachs on the crustacean are relatively less than the former. This review highlights the advancements of RNAi in crustaceans, including the study of methods, gene functions about CHH family, mechanisms of molting and gonadal regulation. In addition, RNAi plays an important role in an antiviral defense in crustacean.

Key words: RNA interference, crustacean, gene function, antiviral defense

邱锡尔, 朱冬发, 周彦琦, 柳志业, 谢熙. 甲壳动物RNAi技术研究与应用进展[J]. 生物技术通报, 2015, 31(3): 57-63.

Qiu Xier, Zhu Dongfa, Zhou Yanqi, Liu Zhiye, Xie Xi. Progress of Research and Application of the RNA Interference Technology in Crustacean[J]. Biotechnology Bulletin, 2015, 31(3): 57-63.

参考文献

[1] 何正波, 陈斌, 冯国忠. 昆虫RNAi技术及其应用[J]. 昆虫知识, 2009, 46(4)：525-532.
[2] Fire A, Xu S, Montgomery MK, et al. Potent and specific genetic inte-rference by double-stranded RNA in Caenorhabditis elegans[J]. Nature, 1998, 391：806-811.
[3] 孙修勤, 闫秀英. RNA干扰技术在水生生物研究中的应用[J]. 中国海洋大学学报, 2008, 38(2)：281-284.
[4] 韩林强, 胜杰, 于凌云, 等. 昆虫RNAi技术及其应用[J]. 昆虫知识, 2009, 46(4)：525-532.
[5] 李珍珠. RNAi技术及其在疾病治疗中的应用[J]. 医学新知杂志, 2008, 18(4)：226-231.
[6] Lu Y, Sun PS. Viral resistance in shrimp that express an antisense Taura syndrome virus coat protein gene[J]. Antiviral Research, 2005, 67(3)：141-146.
[7] Sun PS, Venzon NC, Calderon FRO, et al. Evaluation of methods for DNA delivery into shrimp zygotes of Penaeus(Litopenaeus)vannamei[J]. Aquaculture, 2005, 243(1-4)：19-26.
[8] Ongvarrasopone C, Roshorm Y, Panyim S. A simple and cost effective method to generate dsRNA forRNAi studies in invertebrates[J]. Science Asia, 2007, 33：35-39.
[9] 梁艳, Saksamerprome V, Sritunyalucksan K, 等. 一种大规模制备双链RNA的简单方法及其在斑节对虾中的应用[J]. 水产学报, 2010, 34(7)：1011-1017.
[10] Li DF, Zhang MC, Yang HJ, et al. β-Integrin mediates WSSV infection[J]. Virology, 2007, 368(1)：122-132.
[11] Westenberg M, Heinhuis B, et al. siRNA injection induces seque-nce-independent protection in Penaeus monodon against white spot syndrome virus[J]. Virus Rese, 2005, 114：133-139.
[12] Treerattrakool S, Panyim S, Chan SM, et al. Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interference[J]. FEBS Journal, 2008, 275(5)：970-980.
[13] 李良德, 刘婕, 姜春来, 等. 昆虫RNAi效率的影响因素研究进展[J]. 生物技术通报, 2012(11)：38-42.
[14] Treerattrakool S, Chartthai C, Phrommain N, et al. Silencing of gonad-inhibiting hormone gene expression in Penaeus monodon by feeding with GIH dsRNA-enriched Artemia[J]. Aquaculture, 2013, 404-405(10)：116-121.
[15] 杨济芬, 朱冬发, 沈建明, 等. 甲壳动物高血糖激素家族生理功能研究进展[J]. 动物学杂志, 2009, 44(1)：151-158.
[16] 王晓伟, 张子平, 王艺磊, 等. 虾蟹类卵巢发育调节机制研究进展[J]. 生物技术通报, 2013(7)：29-35.
[17] Bulan P, Meisen I. Two genetic variants of the crustacean hypergl-ycemie hormone(CHH)from the Australian emyfish, Cherax destructor：detection of ehiral isofornm due to post translatlonal modification[J]. Peptides, 2003, 24：1871-1879.
[18] Lugo JM, Morera Y, Rodríguez T, et al. Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti[J]. FEBS J, 2006, 273：5669-5677.
[19] Lee KJ, Kim HW. Molt-inhibiting hormone from the tropical land crab, Gecarcinus lateralis：cloning, tissue expression, and expression of biologically active recombinant peptide in yeast[J]. Gen Comp Endocrinol, 2007, 150(3)：505-513.
[20] Tiu SHK, Chan SM. The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis[J]. FEBS Journal, 2007, 274(17)：4385-4395.
[21] Pamuru RR, Rosen O, et al. Stimulation of molt by RNA interference of the molt-inhibiting hormone in the crayfish Cherax quadricarin-atus[J]. Gen Comp Endocrinol, 2012, 178：227-236.
[22] 王宁, 傅洪拓, 乔慧, 等. 青虾性腺抑制激素GIH基因全长cDNA的克隆及序列分析[C]. 渔业科技创新与发展方式转变-2011年中国水产学会学术年会论文摘要集, 2011.
[23] 管政兵, 水燕, 周鑫, 等. CaM与PMCA可能参与眼柄切除诱导克氏原螯虾卵巢成熟的早期分子过程[J]. 湖北农业科学, 2012, 51(21)：4847-4849.
[24] 李飞, 吴超, 林琼武, 等. 饥饿和切除眼柄对日本囊对虾卵巢发育过程中肝胰腺内生化组分含量的影响[J]. 应用海洋学学报, 2013, 32(4)：524-529.
[25] Treerattrakool S, Panyim S, Udomkit A. Induction of ovarian matu-ration and spawning in Penaeus monodon broodstock by double-stranded RNA[J]. Mar Biotechnol, 2011, 13：163-169.
[26] Lefebvre P, Benomar Y, Staels B. Retinoid X receptors：common heterodimerization partners with distinct functions[J]. Trends in Endocrinology & Metabolism, 2010, 21(11)：676-683.
[27] Riddiford LM, Hinma K, Nelson CA, et al. Insights into the molecu-lar basis of the hormonal control of molting and metamorphosis from Manduca sexta and Drosophila melanogaster[J]. Insect Biochem Mol Biol, 2003, 33：1327-1338.
[28] 李康, 李胜, 曹阳. 蜕皮激素与其受体 EcR-USP 的转录调控机制[J]. 昆虫学报, 2011, 54(8)：933-937.
[29] Wang YH, LeBlanc GA. Interactions of methyl farnesoate and related compounds with a crustacean retinoid X receptor[J]. Mol Cell Endocrinol, 2009, 309(1-2)：109-116.
[30] Priya TAJ, Li F, Zhang J, et al. Molecular characterization and effect of RNA interference of retinoid X receptor(RXR)on E75 and chitinase gene expression in Chinese shrimp Fenneropenaeus chinensis[J]. Comp Biochem Physiol Biochem Mol Biol, 2009, 153(1)：121-129.
[31] 肖业臣, 罗晓斌, 冯佩富, 等. 昆虫几丁质酶的研究进展[J]. 生物技术, 2003, 13(1)：38-39.
[32] Daizo K, Takushi F, Hidehiro F, et al. Effects of 20-hydroxyecdy-sone and KK-42 on chitinase and β-N-acetylglucosaminidase during the larval-pupal transformation of Bombyx mori[J]. Insect Biochemistry, 1991, 21(3)：277-284.
[33] Priya TAJ, Li F, Zhang J, et al. Molecular characterization of an ecdysone inducible gene E75 of Chinese shrimp Fenneropenaeus chinensis and elucidation of its role in molting by RNA interference[J]. Comp Biochem Physiol B Biochem Mol Biol, 2010, 156：149-157.
[34] Katherine AD, Kevin GMK, Jeremy EW. Oxygen-containing analogues of juvenile hormone III[J]. Tetrahedron Letters, 2011, 52(18)：2302-2305.
[35] Laufer H, Ahl JSB, Rotlant G, et al. Evidence that ecdysteroids and methyl farnesoate control allometric growth and differentiation in a crustacean[J]. Insect Biochem Mol Biol, 2002, 32：205-210.
[36] Laufer H, Demir N, Pan X, et al. Methyl farnesoate controls adult male morphogenesis in the crayfish, Procambarus clarkii[J]. Journal of Insect Physiology, 2005, 51(4)：379-384.
[37] Lovett DL, Tanner CA, Glomski K, et al. The effect of seawater composition and osmolality on hemolymph levels of methyl farnes-oate in the green crab Carcinus maenas[J]. Comp Biochem Physiol A Mol Integr Physiol, 2006, 143(1)：67-77.
[38] Hui JHL, Tobe SS, Chan SM, et al. Characterization of the putative farnesoic acid O-methyltransferase(LvFAMeT)cDNA from white shrimp, Litopenaeus vannamei：evidence for its role in molting[J]. Peptides, 2008, 29(2)：252-260.
[39] Das S, Durica DS. Ecdysteroid receptor signaling disruption obstructs blastemal cell proliferation during limb regeneration in the fiddler crab, Uca pugilator[J]. Molecular and Cellular Endocrinology, 2013, 365(2)：249-259.
[40] 李富花, 相建海. 中国对虾促雄腺形态结构和功能的初步研究[J]. 科学通报, 1996, 41(15)：1418-1422.
[41] 吴萍, 楼允东, 邱高峰. 甲壳动物雄性腺研究的进展[J]. 水产学报, 1999, 23(1)：77-81.
[42] Malecha SR, Nevin PA, Ha P, et al. Sex-ratios and sex-determina-tion in progeny from crosses of surgically sex-reversed freshwater prawns, Macrobrachium rosenbergii[J]. Aquaculture, 1992, 105(3)：201-218.
[43] 叶海辉, 李少菁, 王桂忠. 十足目甲壳动物促雄腺研究概述[J]. 动物学杂志, 2001, 36(1)：43-47.
[44] Rosen O, Manor R, Weil S, et al. A sexual shift induced by silencing of a single insulin-like gene in crayfish：ovarian upregulation and testicular degeneration[J]. PloS One, 2010, 5(12)：e15281.
[45] Ventura T, Sagi A. The insulin-like androgenic gland hormone in crustaceans：From a single gene silencing to a wide array of sexual manipulation-based biotechnologies[J]. Biotechnology Advances, 2012, 30(6)：1543-1550.
[46] 马文明, 陈苏, 周蓉. 罗氏沼虾促雄腺提取物对性别的影响及一种促雄性腺类似物的RNA干涉[J]. 中国科技论文在线, 2009, 2(7)：663-668.
[47] 钱叶青. 雄性生殖相关的Kazal型蛋白酶抑制剂和精子明胶酶的结构与功能研究[D]. 杭州：浙江大学, 2011.
[48] 张士璀, 孙旭彤, 李红岩, 等. 卵黄蛋白原研究及其进展[J]. 海洋科学, 2002, 26(7)：32-35.
[49] Tsang WS, Quackenbush LS, Chow BK, et al. Organization of the shrimp vitellogenin gene：Evidence of multiple genes and tissue specific expression by the ovary and hepatopancreas[J]. Gene, 2003, 303(16)：99-109.
[50] Tiu SHK, Benzie J, Chan SM. From hepatopancreas to ovary：Molecular characterization of a shrimp vitellogenin receptor involved in the processing of vitellogenin[J]. Biology of Reproduction, 2008, 79(1)：66-74.
[51] 李维, 王淑红, 王艺磊, 等. 软体动物维甲酸X受体研究进展[J]. 动物学杂志, 2013, 48(4)：655-664.
[52] Nagaraju GPC, Rajitha B, Borst DW. Molecular cloning and sequence of retinoid X receptor in the green crab Carcinus maenas：A possible role in female reproduction[J]. Journal Endocrinology, 2011, 210(3)：379-390.
[53] Tirasophon W, Roshorm Y, Panyim S. Silencing of yellow head virus replication in penaeid shrimp cells by dsRNA[J]. Biochem Biophys Res Commun, 2005, 334(1)：102-107.
[54] 张衡, 谷力, 杨丰. 对虾白斑综合症病毒vp15基因的RNA干扰研究[J]. 台湾海峡, 2012, 31(1)：47-52.
[55] Westenberg M, Heinhuis B, Zuidema D, et al. siRNA injection induces sequence-independent protection in Penaeus monodon against white spot syndrome virus[J]. Virus Research, 2005, 114(1-2)：133-139.
[56] Woramongkolchai N, Supungul P, Tassanakajon A. The possible role of penaeidin5 from the black tiger shrimp, Penaeus monodon, in protection against viral infection[J]. Dev Comp Immunol, 2011, 35(5)：530-536.
[57] Lin YC, Chen JC, Chen YY, et al. Characterization of white shrimp Litopenaeus vannamei integrinβand its role in immunomodulation by dsRNA-mediated gene silencing[J]. Deve Comp Immunol, 2013, 40(2)：167-179.
[58] 肖瑾. 对虾白斑综合症病毒RNAi的抑制[D]. 广州：中山大学, 2007.
[59] Tirasophon W, Yodmuang S, Chinnirunvong W, et al. Therapeutic inhibition of yellow head virus multiplication in infected shrimps by YHV-protease dsRNA[J]. Antiviral Res, 2007, 74：150-155.