一个显性卷叶突变体z2的遗传分析与精细定位

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.014

生物技术通报 ›› 2015, Vol. 31 ›› Issue (6): 100-105.doi: 10.13560/j.cnki.biotech.bull.1985.2015.06.014

一个显性卷叶突变体z2的遗传分析与精细定位

张龙弟¹ 王雁伟² 张治国¹ 吴金霞¹

1.中国农业科学院生物技术研究所，北京 100081；2.首都师范大学，北京 100048

收稿日期:2015-02-04 出版日期:2015-06-19 发布日期:2015-06-20
作者简介:张龙弟，男，硕士研究生，研究方向：植物分子生物学与基因工程；E-mail：zld2011@126.com
基金资助:
中国、巴西作物生物技术与种质资源联合研究项目(2014DFA31550)

Genetic Analysis and Gene Mapping of a Dominant Rolled Leaf Mutant z2 in Rice

Zhang Longdi¹, Wang Yanwei², Zhang Zhiguo¹, Wu Jinxia¹

1.Biotechnology Research Institute，Chinese Academy of Agricultural Science，Beijing 100081；2. Capital Normal University，Beijing 100048

Received:2015-02-04 Published:2015-06-19 Online:2015-06-20

摘要/Abstract

摘要： 叶片的适度卷曲对水稻理想株型育种具有重要意义。在水稻T-DNA插入突变体库中获得一个叶片外卷突变体z2，突变体表型出现在分蘖期；与野生型相比，农艺性状无明显差异，光合作用效率高于野生型。对突变体和野生型的石蜡切片研究表明，突变体叶片泡状细胞数量(8-9个)明显多于野生型(4-5个)。z2卷叶性状遗传稳定，由一对显性核基因控制。以z2纯合突变体为母本，籼稻Dular为父本进行杂交构建F₂代定位群体，利用图位克隆的方法，将该基因初定位于水稻第2号染色体的InDel1812与InDel1870标记之间，物理距离为580 kb。

关键词: 水稻(Oryza sativa L.), 卷叶突变体, 遗传分析, 图位克隆

Abstract: Moderate rolled leaf is significant in ideotype breeding of rice. An outward rolled leaf mutant z2 was discovered from the rice T-DNA insertion mutant library, and its leaves became curly during tillering stage. Compared with the wild type, there was no significant difference in its main agronomic traits, and its photosynthetic was more efficient. Paraffin sections showed that the number of bulliform cells(8-9)in the mutant was higher than that in the wild type(4-5). Through successive selfing, phenotype of z2 was stable and was controlled by a pair of dominant nuclear genes. We made the combination of z2/Dular to establish F2 mapping population, and used map-based cloning method to locate the gene on rice chromosome 2 between maker InDel1812 and InDel1870 with 580 kb physical distance.

Key words: rice(Oryza sativa L.), rolled leaf mutant, genetic analysis, map-based cloning

张龙弟, 王雁伟, 张治国, 吴金霞. 一个显性卷叶突变体z2的遗传分析与精细定位[J]. 生物技术通报, 2015, 31(6): 100-105.

Zhang Longdi, Wang Yanwei, Zhang Zhiguo, Wu Jinxia. Genetic Analysis and Gene Mapping of a Dominant Rolled Leaf Mutant z2 in Rice[J]. Biotechnology Bulletin, 2015, 31(6): 100-105.

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一个显性卷叶突变体z2的遗传分析与精细定位

Genetic Analysis and Gene Mapping of a Dominant Rolled Leaf Mutant z2 in Rice

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