关键词: 表皮毛, GL2, 图位克隆, TTG1, 遗传互作

Abstract: Trichomes widely exist on aboveground parts of terrestrial plants,exhibiting as natural barriers between plants and the environment,and also servering many biological functions. The transcription factor GLABRA 2（GL2）is a key factor regulating trichome initiation and the following developmental processes. Screening and identification of new GL2 interactors will lay a foundation for further uncovering the regulatory molecular mechanisms of trichome development. Through large-scale genetic screening and cloning,a new mutant M12-01 without trichome in the leaves was found,and genetic analysis indicated that the phenotype of M12-01single mutant was controlled by a recessive single nuclear gene. The phenotype of M12-01 single mutant was similar to that of functional deletion mutant of Arabidopsis TTG1 gene. Sequencing TTG1 gene revealed that a single nucleotide replacement from guanine to adenine in +445 of TTG1 gene occurred,which resulted in the encoded glycine changed to arginine. This work confirmed that mutation of TTG1 enhanced the phenotype of gl2-3 mutant,and genetic interaction between gene GL2 and TTG1,which provides new genetic materials for further studying the molecular mechanism of GL2 regulation on the development of plant trichome.

Key words: trichome, GL2, map-based cloning, TTG1, genetic interaction