生物技术通报 ›› 2015, Vol. 31 ›› Issue (6): 144-150.doi: 10.13560/j.cnki.biotech.bull.1985.2015.06.023

• 研究报告 • 上一篇    下一篇

低聚半乳糖对肠道益生菌产胞外多糖作用的研究

辛跃强 梁荣荣 王瑞明   

  1. 齐鲁工业大学生物工程学院 山东省微生物工程重点实验室,济南 250353
  • 收稿日期:2014-10-19 出版日期:2015-06-19 发布日期:2015-06-20
  • 作者简介:辛跃强,男,硕士研究生,研究方向:微生物酶技术;E-mail:qiangzi890125@163.com

Effects of Galactooligosaccharide on Exopolysaccharide Produced by Intestinal Probiotics

Xin Yueqiang, Liang Rongrong, Wang Ruiming   

  1. Department of Biotechnology,Qilu University of Technology,Key Laboratory for Microbial Engineering in Shandong Province,Ji'nan 250353
  • Received:2014-10-19 Published:2015-06-19 Online:2015-06-20

摘要: 旨在研究低聚半乳糖对肠道益生菌产胞外多糖的作用。以低聚半乳糖、葡萄糖、半乳糖和乳糖4种碳源作比较,分别从碳源的利用、胞外多糖的产量和种类及其对有害菌的黏附作用进行研究。结果显示,低聚半乳糖不仅能促进长双歧杆菌和植物乳杆菌的增殖,而且有利于胞外多糖的产生,其含量分别为208.78 μg/mL和192.78 μg/mL,产生的胞外多糖种类较其他3种碳源更多,对肠道有害菌大肠杆菌具有明显黏附作用。结果证明,与其他3种碳源相比,低聚半乳糖能促进植物乳杆菌和两歧双歧杆菌生成更多的胞外多糖。

关键词: 低聚半乳糖, 胞外多糖, 益生菌

Abstract: The aim of the study is to investigate the effects of galactooligosaccharide on the yield of exopolysaccharides produced by intestinal probiotics. Using galactooligosaccharide, glucose, galactose and lactose as carbon sources, the utilization of carbon sources, the yield and varieties of exopolysaccharides and the adhesion of harmful bacteria were studied. Results indicated that galactooligosaccharide not only promoted reproduction of Bifidobacterium longum and Lactobacillus plantarum, but also improved the yield of exopolysaccharides with 208.78 μg/mL and 192.78 μg/mL respectively. Varieties of exopolysaccharides produced while using galactooligosaccharide as carbon source were more than other 3 ones, and the adhesion to harmful bacteria such as Escherichia coli was obvious. It is proved that galactooligosaccharide could promote B. longum and L. plantarum to produce more exopolysaccharides compared with the other 3 carbon sources.

Key words: galactooligosaccharide, exopolysaccharide, intestinal probiotics