黄芩醇提物对副溶血性弧菌抑制机制的研究

doi:10.13560/j.cnki.biotech.bull.1985.2015.08.023

摘要/Abstract

摘要： 从4种常见中药中筛选出对副溶血性弧菌有较好抑制作用的中药,并对其抑制机制进行研究。采用牛津杯法及液体倍比稀释法研究了中药醇提取物对副溶血性弧菌的敏感性;通过生长曲线和杀菌曲线、电镜观察以及SDS-PAGE等方法探讨黄芩醇提物的抑菌作用及机理。结果显示,4种中药醇提物对副溶血性弧菌表现出不同的抑菌活性,其中黄芩醇提物对副溶血性弧菌的抑制作用最强,抑菌圈为22.00±1.00 mm,MIC和MBC均为3.91 mg/mL。经MIC浓度处理后,细菌经过较长一段时间的延迟期,也能进入正常的对数期和稳定期;菌体表面变粗糙,出现裂解,菌液的蛋白含量明显升高,但是菌体的可溶性蛋白变化较小。结果表明,黄芩醇提物对副溶血性弧菌有很强的抑制效果,其作用是破坏细胞膜的完整性导致菌体裂解死亡。

关键词: 黄芩, 副溶血性弧菌, 醇提物, 抑菌机理

Abstract: The goal of this work is to screen one with strong antibacterial activities against Vibrio parahaemolyticus from 4 traditional Chinese medicines（TCMs）, and study the underlying mechanisms. The sensitivity of ethanol-extracts from TCMs to Vibrio parahaemolyticus was determined by Oxford cup assay and liquid double dilution method. The antibacterial mechanism was investigated by measuring growth curve and bactericidal curve, electron microscope examination, and SDS-PAGE analysis. The results showed that different ethanol-extracts from 4 TCMs had different antibacterial activities upon V. parahaemolyticus, among them the ethanol-extract from S. baicalensis had the best antibacterial activity with an inhibition zone diameter of 22.00 ± 1.00 mm. Both the minimum inhibitory concentration（MIC）and minimum bactericidal concentration（MBC）of the extract against V. parahaemolyticus were 3.91 mg/mL. V. parahaemolyticus treated with Scutellaria extract in MIC still entered a normal log and stationary phase after a longer lag phase. Bacterial surface became rough, showing a sign of cell lysis. Protein concentration in the supernatant increased significantly. However, the concentration of soluble proteins changed lightly after the treatment of Scutellaria extract. Conclusively, Scutellaria extract has a significant inhibition effect against V. parahaemolyticus by destroying the integrity of cell membrane and causing cell lysis.

Key words: Scutellaria baicalensis, Vibrio parahaemolyticus, ethanol-extract, antibacterial mechanism.

谢丽玲, 彭齐, 蔡链纯, 周亮, 朱炎坤, 韩光耀. 黄芩醇提物对副溶血性弧菌抑制机制的研究[J]. 生物技术通报, 2015, 31(8): 159-165.

Xie Liling, Peng Qi, Cai Lianchun, Zhou Liang, Zhu Yankun, Han Guangyao. A Study on Antibacterial Mechanisms of Ethanol-extracts from Scutellaria baicalensis Against Vibrio parahaemolyticus[J]. Biotechnology Bulletin, 2015, 31(8): 159-165.

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