稻瘟病菌机械敏感性离子通道的鉴定及功能分析

doi:10.13560/j.cnki.biotech.bull.1985.2016-1209

生物技术通报 ›› 2017, Vol. 33 ›› Issue (6): 97-103.doi: 10.13560/j.cnki.biotech.bull.1985.2016-1209

稻瘟病菌机械敏感性离子通道的鉴定及功能分析

陶尚琨, 王国梁, 刘文德

中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京 100193

收稿日期:2017-01-09 出版日期:2017-06-26 发布日期:2017-06-19
作者简介:陶尚琨,男,硕士研究生,研究方向：分子植物病理学;E-mail：shangkuntao@126.com
基金资助:
国家自然科学基金项目（31422045）

Identification and Functional Analysis of Mechanosensitive Channels in Magnaporthe oryzae

TAO Shang-kun, WANG Guo-liang, LIU Wen-de

State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193

Received:2017-01-09 Published:2017-06-26 Online:2017-06-19

摘要/Abstract

摘要： 稻瘟病是水稻上危害最为严重的病害之一,其病原菌为子囊菌门稻瘟病菌。作为模式真菌,研究稻瘟病菌的致病机理不仅能为其病害防治提供理论依据,也给其它病原真菌提供借鉴意义。离子通道是细胞内外物质交换的窗口,具有成为防治靶标的潜力,对病害防控具有重要意义。机械敏感性离子通道是一类通过感受细胞膜张力变化来实现胞内外机械信号转导的离子通道。运用生物信息学鉴定了稻瘟病菌的3条机械敏感性离子通道蛋白MoMsc1,MoMid1和MoYvc1,进化分析表明它们在真菌里具有保守性。还对稻瘟病菌的小电导率机械敏感性离子通道蛋白MoMsc1进行了功能分析,该基因沉默并未影响其生长发育、外界胁迫、细胞壁完整性及致病力。

关键词: 稻瘟菌, 机械敏感性离子通道, 小电导率机械敏感性离子通道

Abstract: Rice blast disease,caused by ascomycete Magnaporthe oryzae,is one of the most devastating diseases threatening rice production. Understanding its pathogenic mechanism not only provides strategies for disease control,but also generates new knowledge of fungal pathogenesis. Mechanosensitive channels,the window of material exchange,can be the biocontrol targets for disease control. These channels can sense changes of membrane tension and transduce this signal into intracellular environment. In this study,we identified three mechanosensitive channels（MoMsc1,MoMid1,and MoYvc1）in M. oryzae by bioinformatics method. The results showed that the three genes were evolutionarily conserved in fungi. Furthermore,we discovered that silence of MoMsc1,encoding the putative gene of mechanosensitive channel of small conductance in M. oryzae,did not affect its vegetative growth,asexual development,stress response,cell wall integrity and virulence.

Key words: Magnaporthe oryzae, mechanosensitive channel, mechanosensitive channel of small conductance

陶尚琨, 王国梁, 刘文德. 稻瘟病菌机械敏感性离子通道的鉴定及功能分析[J]. 生物技术通报, 2017, 33(6): 97-103.

TAO Shang-kun, WANG Guo-liang, LIU Wen-de. Identification and Functional Analysis of Mechanosensitive Channels in Magnaporthe oryzae[J]. Biotechnology Bulletin, 2017, 33(6): 97-103.

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稻瘟病菌机械敏感性离子通道的鉴定及功能分析

Identification and Functional Analysis of Mechanosensitive Channels in Magnaporthe oryzae

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