极端嗜酸热古菌Acidianus manzaensis硫氧化模型构建

doi:10.13560/j.cnki.biotech.bull.1985.2017-0620

生物技术通报 ›› 2018, Vol. 34 ›› Issue (5): 179-186.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0620

极端嗜酸热古菌Acidianus manzaensis硫氧化模型构建

朱薇¹,马亚龙²

1. 湖南工业大学,株洲 412000;
2. 中南大学资源加工与生物工程学院,长沙 410083

收稿日期:2017-07-25 出版日期:2018-05-26 发布日期:2018-06-07
作者简介:朱薇,女,博士,副教授,研究方向：生物冶金;E-mail：greatcsu@163.com
基金资助:
国家自然科学基金项目（51404104）,湖南省自然科学基金项目（2015JJ3062）,湖南省教育厅项目（14C0341）

Sulfur Oxidation Model Construction of Extremely Thermoacidophilic Archaea Acidianus manzaensis

ZHU Wei¹, MA Ya-long²

1. Hunan Industrial University,Zhuzhou 412000;
2. School of Minerals Processing and Bioengineering,Central South University,Changsha 410083

Received:2017-07-25 Published:2018-05-26 Online:2018-06-07

摘要/Abstract

摘要： 为了解极端嗜酸热古菌硫氧化代谢途径,基于Acidianus manzaensis YN-25全基因组信息,通过NCBI数据库比对初步筛选了20个可能与硫氧化相关的基因,并通过实时定量PCR（RT-qPCR）比较了所筛选基因在以单质硫（S⁰）和亚铁（Fe²⁺）两种不同能源底物培养下的表达差异。结果表明,A. manzaensis菌中存在至少15个与硫氧化相关的基因,经过比对分析,它们包括5个编码氧化单质硫（S⁰）及含硫中间产物的酶基因、4个编码末端氧化酶基因、1个编码硫酸根转运蛋白酶基因、1个编码电子传递蛋白基因,以及4个编码与硫氧化密切相关的硫还原蛋白家族（Sulfur reduction protein）的dsrE基因。基于上述实验分析结果,拟构建极端嗜酸热古菌A. manzaensis的硫氧化模型,即胞外的S⁰跨膜转运进入细胞内后,经硫氧化蛋白（SOR）氧化还原生成S₂O₃^2-,SO₃^2- 和 H₂S等含硫中间产物。接着,细胞内通过其他相关硫氧化酶的作用将这些中间产物进一步氧化,并将氧化得到的电子传递给细胞膜上的氧化型醌（Q²⁺）,使其形成还原型的醌（QH₂）,QH₂最终被末端氧化酶氧化形成NADH和ATP,从而为细胞生长提供能量。

关键词: 基因组分析, 极端嗜酸热古菌, Acidianus manzaensis, 硫氧化模型

Abstract: To understand the metabolic pathway of sulfur oxidation in extremely thermoacidophilic archaea,20 possible genes related to sulfur oxidation were preliminarily screened by NCBI database alignment based on the complete genome sequence of Acidianus manzaensis YN-25. Quantitative real-time PCR（RT-qPCR）was used to analyze the differential expressions of the screened genes under two different energy substrates,elemental sulfur（S⁰）and iron（Fe²⁺）. The results indicated that 15 genes were associated with sulfur oxidation from the compartive analysis of the experiment results ,including 5 genes encoding the enzymes of oxidizing S⁰ and sulfur-containing intermediates,4 genes encoding terminal oxidase,1 gene encoding sulfate transporter permease,1 gene encoding electron transfer protein,and 4 dsrE genes encoding sulfur reduction protein family closely related to sulfur oxidation. Based on the above result of analysis,a sulfur oxidation model of A. manzaensis was presented as below. Extracellular S⁰ was transported into the cell through membrane,and reduced by sulfur oxygenase-reductase（SOR）to be intermediates such as S₂O₃^2-,SO₃^2- and H₂S. Following,these sulfur-containing intermediates were further oxidized by relevant enzymes inside cells,and the electrons from the oxidation were transferred to oxidized quinone（Q²⁺）on the cell membrane,from which the reduced quinone（QH₂）was formed. QH₂ were eventually oxidized by terminal oxidase to be NADH and ATP providing energy for the growth of cells.

Key words: genome analysis, extremely thermoacidophilic archaea, Acidianus manzaensis, sulfur oxidation model

朱薇,马亚龙. 极端嗜酸热古菌Acidianus manzaensis硫氧化模型构建[J]. 生物技术通报, 2018, 34(5): 179-186.

ZHU Wei, MA Ya-long. Sulfur Oxidation Model Construction of Extremely Thermoacidophilic Archaea Acidianus manzaensis[J]. Biotechnology Bulletin, 2018, 34(5): 179-186.

参考文献

[1] Dopson M, Johnson DB.Biodiversity, metabolism and applications of acidophilic sulfur-metabolizing microorganisms[J]. Environmental Microbiology, 2012, 14(10):2620-2631.
[2] 周吉奎, 钮因健. 硫化矿生物冶金研究进展[J]. 金属矿山, 2015(4):24-30.
[3] 周洪波, 毛峰, 王玉光. 嗜酸微生物与生物冶金技术[J]. 矿物岩石地球化学通报, 2015(2):269-276.
[4] Li Y, Kawashima N, Li J. A review of the structure,fundamental mechanisms and kinetics of the leaching of chalcopyrite[J]. Advances in Colloid & Interface Science, 2013, 197-198(9):1-32.
[5] Friedrich CG, Bardischewsky F, Rother D, et al.Prokaryotic sulfur oxidation[J]. Curr Opin Microbiol, 2005, 8(3):253-259.
[6] Friedrich CG, Rother D, et al.Oxidation of reduced inorganic sulfur compounds by bacteria:emergence of a common mechanism?[J]. Appl Environ Microbiol, 2001, 67(7):2873-2882.
[7] Ding J, Zhang R, et al.A novel acidophilic, thermophilic iron and sulfur-oxidizing archaeon isolated from a hot spring of tengchong, yunnan, China[J]. Braz J Microbiol, 2011, 42(2):514-525.
[8] He H, Xia J, Yang Y, et al.Sulfur speciation on the surface of chalcopyrite leached by Acidianus manzaensis[J]. Hydrometallurgy, 2009, 99(1-2):45-50.
[9] Liang CL, Xia JI, et al.Effect of sodium chloride on sulfur speciation of chalcopyrite bioleached by the extreme thermophile Acidianus manzaensis[J]. Bioresour Technol, 2012, 110(2):462-467.
[10] Ma YL, Xia JL, Yang Y, et al. Complete genome sequence of the extremely thermoacidophilic archaeon Acidianus manzaensis YN-25[J]. Genome Announcements, 2017, 5(25), pii:e00438-17.
[11] 彭安安, 刘红昌, 聂珍媛, 等. 硫酸钡比浊法的改良及其在生物冶金浸出体系硫酸根浓度测定中的应用[J]. 中南大学学报:自然科学版, 2013, 44(6):2186-2191.
[12] Liu HC, Xia JL, Nie ZY, et al.Iron L-edge and sulfur K-edge XANES spectroscopy analysis of pyrite leached by Acidianus manzaensis[J]. Transactions of Nonferrous Metals Society of China, 2015, 25(7):2407-2414.
[13] Peng AA, Xia JL, et al.Thiol-rich proteins play important role in
adhesion and sulfur oxidation Process of Acidithiobacillus ferroxid-ans[J]. Advanced Materials Research, 2013, 825:137-140.
[14] Yu NY, Wagner JR, Laird MR, et al.PSORTb 3. 0:improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes[J]. Bioinformatics, 2010, 26(13):1608-1615.
[15] 彭安安. 嗜酸硫氧化细菌元素硫活化氧化机制研究[D]. 长沙:中南大学, 2012.
[16] Almárcegui RJ, Navarro CA, Paradela A, et al.Response to copper of Acidithiobacillus ferrooxidans ATCC 23270 grown in elemental sulfur[J]. Research in Microbiology, 2014, 165(9):761-772.
[17] Zhu W, Xia JL, Peng AA, et al. Characterization of apparent sulfur oxidation activity of thermophilic archaea in bioleaching of chalcopyrite[J]. Transactions of Nonferrous Metals Society of China, 2013, 23(8)2383-2388.
[18] You XY, Liu C, Wang SY, et al.Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles[J]. Extremophiles, 2011, 15(4):487-497.
[19] Kletzin A, Urich T, Müller F, et al.Dissimilatory oxidation and reduction of elemental sulfur in thermophilic archaea[J]. Journal of Bioenergetics & Biomembranes, 2004, 36(1):77-91.
[20] Rohwerder T, Sand W.Oxidation of inorganic sulfur compounds in acidophilic prokaryotes[J]. Engineering in Life Sciences, 2010, 7(4):301-309.
[21] Rohwerder T, Sand W.The sulfane sulfur of persulfides is the actual substrate of the sulfur-oxidizing enzymes from Acidithiobacillus and Acidiphilium spp.[J]. Microbiology, 2003, 149(Pt 7):1699.
[22] Liu HC, Xia JL, et al.Differential expression of extracellular thiol
groups of moderately thermophilic Sulfobacillus thermosulfidooxi-dans and extremely thermophilic Acidianus manzaensis grown on S⁰ and Fe²+[J]. Arch Microbiol, 2015, 197(6):823.
[23] Quatrini R, Appia-Ayme C, et al.Extending the models for iron and sulfur oxidation in the extreme Acidophile Acidithiobacillus ferrooxidans[J]. BMC Genomics, 2009, 10(1):394.
[24] Kletzin A.Oxidation of sulfur and inorganic sulfur compounds in Acidianus ambivalens[M]// Microbial Sulfur Metabolism. Springer Berlin Heidelberg, 2008:184-201.
[25] Komorowski L, Verheyen W, Schäfer G.The archaeal respiratory supercomplex SoxM from S. acidocaldarius combines features of quinole and cytochrome c oxidases[J]. Biological Chemistry, 2002, 383(11):1791-1799.

极端嗜酸热古菌Acidianus manzaensis硫氧化模型构建

Sulfur Oxidation Model Construction of Extremely Thermoacidophilic Archaea Acidianus manzaensis

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

本文评价

[1]	文畅, 刘晨, 卢诗韵, 许忠兵, 艾超凡, 廖汉鹏, 周顺桂. 一株新的多重耐药福氏志贺菌噬菌体生物学特性及基因组分析[J]. 生物技术通报, 2022, 38(9): 127-135.
[2]	李霁虹, 荆玉玲, 马桂珍, 郭荣君, 李世东. 无色杆菌77的基因组构成及其趋化和耐药特性[J]. 生物技术通报, 2022, 38(9): 136-146.
[3]	刘警鞠, 张雨森, 陈娟, 孙炳达, 赵国柱. 曲霉属的现代分类命名研究进展[J]. 生物技术通报, 2022, 38(7): 109-118.
[4]	王娅丽, 朱姗姗, 杨峰山, 马玉堃, 付海燕, 刘春光. 莠去津降解菌泛基因组测序及比较基因组分析[J]. 生物技术通报, 2019, 35(7): 90-99.
[5]	罗燕,刘小刚,周志钦. 植物糖基转移酶基因的分离方法及其生物学功能研究进展[J]. 生物技术通报, 2016, 32(12): 34-39.
[6]	马亚龙,刘红昌,夏金兰,杨云,聂珍媛，. 极端嗜酸热古菌Acidianus manzaensis胞外硫活化蛋白质基因的筛选及鉴定[J]. 生物技术通报, 2016, 32(12): 143-151.
[7]	杨云, 刘红昌, 夏金兰, 马亚龙, 聂珍媛. 极端嗜酸热古菌Acidianus manzaensis膜蛋白提取方法的建立及应用[J]. 生物技术通报, 2016, 32(11): 130-136.
[8]	孙雷心. 连锁图谱开创遗传研究新领域[J]. , 1995, 0(01): 10-11.
[9]	孙国凤;. James Watson向消极对待基因组分析的人发出警告[J]. , 1990, 0(08): 23-24.
[10]	王旭宁;. TRW数据寻查技术[J]. , 1990, 0(04): 7-7.