CO2浓度对雨生红球藻生理生化指标的影响

doi:10.13560/j.cnki.biotech.bull.1985.2017-0669

摘要/Abstract

摘要： 为了探讨雨生红球藻对不同CO₂浓度的响应,利用生理生化测定方法比较了两种CO₂浓度对雨生红球藻生长、色素含量、叶绿素荧光参数和两种碳代谢相关酶的影响。结果显示在雨生红球藻的绿色营养阶段,4倍空气CO₂浓度下培养的藻细胞密度是空气CO₂浓度下的3.08倍（第10 天）,与空气CO₂相比,较高CO₂培养藻的叶绿素和类胡萝卜素含量、胞外碳酸酐酶（CA）活性降低,非光化学淬灭（NPQ）显著升高,最大光能转化效率（Fv/Fm）、实际光化学量子效率（Ф_PSII）多显著升高,而光化学淬灭（qP）、核酮糖-1,5-二磷酸羧化酶/加氧酶（Rubisco）活性无显著差异。在雨生红球藻的红色孢子阶段,4倍空气CO₂培养下的藻细胞密度显著降低,但虾青素含量提高了20.23%（第8 天）。以上研究表明可以通过适当提高CO₂浓度来促进雨生红球藻的生长和虾青素的积累。

关键词: 雨生红球藻, CO₂, 藻细胞密度, 色素含量, 叶绿素荧光参数

Abstract: In order to explore the response of microalga Haematococcus pluvialis to different CO₂ concentrations,the effects of two kinds of CO₂ concentrations on the algal growth,pigment content,chlorophyll fluorescence parameter and activities of two carbon metabolism-related enzymes,were investigated by the physiological and biochemical methods. Results showed that at the green stage,compared to the air CO₂,the growth rate of H. pluvialis increased 3.08 times（10 th）under quadruple air CO₂ condition;the chlorophyll and carotenoid content,and the extracellular carbonic anhydrase activity all decreased;non-photochemical quenching（NPQ）,most of quantum yield of photo-chemical electron transport_（Ф_PSII）,and maximal photo-chemical efficiency of PSII（F_v/F_m）almost significantly increased,whereas the photo-chemical quenching（qP）and activity of ribulose-1,5-bisphosphate carboxylase/oxygenase（Rubisco）differed insignificantly.At the red stage,the algae cultured with quadruple air CO₂ grew slower,however,their astaxanthin content increased by 20.23%（8 d）of that under air CO₂condition. The study indicated that appropriately high CO₂concentration promoted its growth rate and astaxanthin accumulation in H.pluvialis.

Key words: Haematococcus pluvialis, CO₂, algal cell density, pigment content, chlorophyll fluorescence parameter

陈佳妮, 林丽春, 徐年军, 张琳, 孙雪. CO₂浓度对雨生红球藻生理生化指标的影响[J]. 生物技术通报, 2018, 34(1): 239-246.

CHEN Jia-ni, LIN Li-chun, XU Nian-jun, ZHANG Lin, SUN Xue. Effects of CO₂Concentrations on the Physiological and Biochemical Parameters of Haematococcus pluvialis[J]. Biotechnology Bulletin, 2018, 34(1): 239-246.

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CO₂浓度对雨生红球藻生理生化指标的影响

Effects of CO₂Concentrations on the Physiological and Biochemical Parameters of Haematococcus pluvialis

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