酿酒酵母PMT1与PMT3双基因缺失对细胞寿命的影响

doi:10.13560/j.cnki.biotech.bull.1985.2017-0847

生物技术通报 ›› 2018, Vol. 34 ›› Issue (4): 139-143.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0847

酿酒酵母PMT1与PMT3双基因缺失对细胞寿命的影响

崔红晶, 周涛, 邱堃佩, 宋浩昌, 刘新光

广东医科大学衰老研究所广东省医学分子诊断重点实验室东莞市衰老与抗衰老重点实验室,东莞 523808

收稿日期:2017-10-13 出版日期:2018-04-20 发布日期:2018-05-04
作者简介:崔红晶,女,博士,研究方向：细胞衰老的分子机制;E-mail：cjingjing79@126.com
基金资助:
国家自然科学基金项目（31701050,81671399）,广东省医学科学技术研究基金项目（A2016257,B2014299）,东莞市医疗卫生科技计划一般项目（2016105101289）,东莞市国际科技合作（含港澳台）项目（201650812001）

Effects of PMT1 and PMT3 Double-gene Deficiency on the Lifespan of Saccharomyces cerevisiae

CUI Hong-jing, ZHOU Tao, QIU Kun-pei, SONG Hao-chang, LIU Xin-guang

Institute of Aging Research,Guangdong Medical University; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics,Guangdong Medical University; Aging and Anti-aging Key Laboratory of Dongguan,Guangdong Medical University,Dongguan 523808

Received:2017-10-13 Published:2018-04-20 Online:2018-05-04

摘要/Abstract

摘要： 研究蛋白质O-甘露糖转移酶（Protein O-mannosyltransferase,PMT）家族中PMT3基因缺失,以及PMT1与PMT3双基因缺失对酵母细胞复制性寿命的影响。采用一步基因置换法构建PMT3基因缺失酵母菌株（pmt3Δ）,基于基因同源重组的原理,构建PMT1和PMT3双基因缺失酵母菌株（pmt1Δpmt3Δ）;显微镜下分离和计数酵母子细胞的数目,统计酵母细胞复制性寿命。与野生型酵母细胞的平均复制性寿命（26代）比较,pmt3Δ菌株（24代,P > 0.05）和pmt1Δpmt3Δ菌株（25代,P > 0.05）的平均寿命均无明显变化,差异无统计学意义。酵母PMT3单基因缺失,以及PMT1和PMT3双基因缺失均不影响细胞的复制性寿命。

关键词: 酿酒酵母, 细胞寿命, 出芽, 糖基化

Abstract: We herein aimed to explore the effects of the PMT3 of protein O-mannosyltransferase（PMT）single-gene deficiency,and PMT1 and PMT3 double-gene deficiency on the replicative lifespan of Saccharomyces cerevisiae. PMT3 deficiency strain（pmt3Δ）was constructed through polymerase chain reaction（PCR）-mediated one-step gene disruption,and PMT1 and PMT3 double-gene deficiency strain（pmt1Δpmt3Δ）was prepared based on homologous recombination. The replicative lifespan of yeast cells were counted by separating daughter cells from mother cells using a manual micromanipulator equipped with a fiber-optic needle under the microscope. The replicative lifespan of the pmt3Δ yeast strain（24 generations）and pmt1Δpmt3Δ strain（25 generations,P > 0.05）exhibited no significant difference,compared with that of wild-type yeast strain（26 generations）（P > 0.05）,i.e.,there was no statistical significance among the differences. Conclusively,PMT3 single-gene deficiency and PMT1 and PMT3 double-gene deficiency exert no evident effects on the lifespan of yeast cells.

Key words: Saccharomyces cerevisiae, cell lifespan, budding, glycosylation

崔红晶, 周涛, 邱堃佩, 宋浩昌, 刘新光. 酿酒酵母PMT1与PMT3双基因缺失对细胞寿命的影响[J]. 生物技术通报, 2018, 34(4): 139-143.

CUI Hong-jing, ZHOU Tao, QIU Kun-pei, SONG Hao-chang, LIU Xin-guang. Effects of PMT1 and PMT3 Double-gene Deficiency on the Lifespan of Saccharomyces cerevisiae[J]. Biotechnology Bulletin, 2018, 34(4): 139-143.

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酿酒酵母PMT1与PMT3双基因缺失对细胞寿命的影响

Effects of PMT1 and PMT3 Double-gene Deficiency on the Lifespan of Saccharomyces cerevisiae

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