产丁醇梭菌基因改造的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017.01.011

摘要/Abstract

摘要： 产丁醇梭菌作为丁醇发酵的主要生产菌，近年来成为研究的热点，而丁醇作为新型可再生能源，其优势远大于乙醇，因此，对产丁醇梭菌的基因改造研究具有重大意义。从关键基因、糖酵解途径和丁醇耐受性的角度出发，介绍了近几年来对产丁醇梭菌基因改造的研究进展。讨论了目前研究存在的问题，并对如何提高丁醇产量提出一些见解，旨为广大研究者提供新的思路。

关键词: 产丁醇梭菌, ABE发酵, EMP, 基因过表达, 基因失活, 丁醇耐受性

Abstract: Butanol-producing Clostridia as butanol fermentation strains have been studied frequently in recent years. As a new renewable energy sources,butanol has obviously more advantages than ethanol. Therefore,it is of great significance to study gene modification of butanol-producing Clostridia. In this review,from three aspects of key genes,glycolytic pathway,and butanol tolerance,we introduced the latest research progress on gene modification of butanol-producing Clostridia. Meanwhile,we discussed the issues in the current research,and put forward suggestions on how to improve the butanol yield,aiming at providing some new ideas for the researchers.

Key words: butanol-producing Clostridia, ABE fermentation, EMP, gene overexpress, gene inactivation, butanol tolerance

张超，王义强，王启业，黄瑞春，米小琴. 产丁醇梭菌基因改造的研究进展[J]. 生物技术通报, 2017, 33(1): 106-113.

ZHANG Chao, WANG Yi-qiang, WANG Qi-ye, HUANG Rui-chun, MI Xiao-qin. Research Progress for Genetic Modification of Butanol-producing Clostridia[J]. Biotechnology Bulletin, 2017, 33(1): 106-113.

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