关键词: 10-脱乙酰巴卡亭III（10-DAB）, 巴卡亭III, 成团泛菌, 生物转化, 工艺优化

Abstract: 10-Deacetyl baccatin III（10-DAB）is the synthetic precursor of docetaxel-a taxanes drug with anti-tumor activity. The biotransformation technology conditions for 10-DAB production from baccatin III by Pantoea agglomerans P2-A-8 was optimized by response surface methodology（RSM）. Using Box-Behnken test and variance analysis,the transformation conditions for baccatin III to 10-DAB were optimized from the followings：cell culture time,cell concentration OD ₆₀₀,substrate final concentration,transformation temperature,and transformation time. The optimized biotransformation conditions were as follows：after the second stage cultivation for 12 h,cells were transferred and the initial OD₆₀₀ of cells was adjusted to 3.0 and the final concentration of baccatin III solution（dissolved in methanol）was 0.007 mg/mL The biotransformation was performed at 32℃ on a rotary shaker（200 r/min）for 4 d. Under the above optimized conditions,the yield of 10-DAB reached 24.5%,which increased by 446.8% compared with that before optimization. This study proved that applying RSM for modeling of 10-DAB production from baccatin III by Pantoea agglomerans P2-A-8 was reasonable and feasible. This result has important application value for docetaxel biosynthesis.

Key words: 10-DAB, baccatin III, Pantoea agglomerans, biotransformation, technology optimization