生物转化能源草制取纤维素乙醇的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017.05.007

摘要/Abstract

摘要： 随着化石燃料的日益枯竭和环境污染的日益加剧,寻找一种绿色能源以代替化石能源成为当今世界迫在眉睫的任务。清洁燃料当中的生物乙醇具有车用价值,可作为化石能源的替代品而受到研究学者的广泛关注。而草本能源植物的生物转化被认为是生物质能源产业化发展的最有效途径之一。能源草作为木质纤维素原料之一,由于其具有生长快,产量高,抗性强等优势而备受瞩目。详细论述了近期国内外以能源草为底物进行纤维素乙醇的生物转化研究进展,从纤维素原料预处理到乙醇发酵工艺等各方面的进展及存在的问题,并对木质纤维素制取生物质能源的生物转化效率,以及全纤维素组分的多级利用进行了简单阐述,以期找出一条产业化生产纤维素乙醇的最优生产模式。

关键词: 生物质能源, 能源草, 生物转化, 纤维素乙醇

Abstract: With the increasing depletion of fossil fuels and the deterioration of environmental pollution,seeking a green energy to replace fossil energy is an urgent task for the world. Bio-ethanol as a clean energy presents the value for vehicle,and can be used as a clean substitute of fossil energy,which is widely concerned by the researchers. And bio-conversion of grass energy is recognized as the most efficient measure for the industrialization of biomass energy. Energy grass as one of lignocellulose materials,because of its fast growth,high yield,strong resistance and other advantages,has been attracted much attention. This paper discusses the recent progresses on the bio-conversion of energy grass as substrate for cellulosic ethanol,i.e.,advances and existing problems in varied aspects from the pretreatment of raw cellulose to the fermentation process of ethanol,bioconversion efficiency in the bioenergy production from lignocellulose,and conprehensive utilizations of all components of lignocellulose are briefly discussed,aiming at finding out an optimal production mode for the industrial production of cellulosic ethanol.

Key words: biomass energy, energy grass, bio-conversion, cellulosic ethanol

白龙,李春美,吕途,杜颖,杨玥,田沈. 生物转化能源草制取纤维素乙醇的研究进展[J]. 生物技术通报, 2017, 33(5): 50-56.

BAI Long LI Chun-mei LÜ Tu DU Ying YANG Yue TIAN Shen. Research Progress on the Biological Conversion of Energy Grass to Cellulosic Ethanol[J]. Biotechnology Bulletin, 2017, 33(5): 50-56.

参考文献

[1] 汪辉, 周禾, 高凤芹等. 能源草发酵产沼气的研究进展[J]. 山东农业科学, 2014, 46（3）：135-139.
[2] Gallego L, Escobar A, Penuela M. King Grass：A promising material for the production of second-generation butanol[J]. Fuel, 2015, 143：399-403.
[3] 牛红志, 李连华, 孔晓英, 等. 三种能源草厌氧发酵制备生物燃气初步研究[J]. 新能源展, 2015（3）：192-196.
[4] 高瑞芳, 张建国. 能源草研究进展[J]. 草原与草坪, 2013, 1（33）：89-96.
[5] 闫莉, 吕惠生, 张敏华. 纤维素乙醇生产技术及产业化进展[J]. 酿酒科技, 2013（10）：80-84.
[6] Ding SY, Himmel ME. The maize primary cell wall microfibril：a new model derived from direct visualization[J]. Agric Food Chem, 2006, 54（3）：597-606.
[7] 贾晶霞, 梁宝忠, 王艳红, 等. 不同汽爆预处理对干玉米秸秆青贮效果的影响[J]. 农业工程学报2013, 29（20）：192-198.
[8] Kaien R, Fumitaka S, Jun A, et al. Biomass yield and nitrogen use efficiency of cellulosic energy crops for ethanol production[J]. Biomass and Bioenergy, 2012, 37：330-334.
[9] 阎金龙, 王艳君, 马烽, 等. 木质纤维素原料预处理技术相关研究进展[J]. 中国酿造, 2013, 32（11）：7-10.
[10] Vasco-correa J, Li YB. Solid-state anaerobic digestion of fungal pretreated Miscanthus sinensis harvested in two different seasons[J]. Bioresource Technology, 2015, 185：211-217.
[11] Scholl AL, Menegol D, Pitarelo AP, et al. Ethanol production from sugars obtained during enzymatic hydrolysis of elephant grass（Pennisetum purpureum, Schum. ）pretreated by steam explosion[J]. Bioresource Technology, 2015, 192：228-237.
[12] Alvira P, Tomás-Pejó E, Ballesteros M, et al. Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis：a review[J]. Bioresource Technology, 2010, 101（13）：4851-4861.
[13] Conde MC, Jiménez GA, Halwagi EM. A comparison of pretreatment methods for bioethanol production from lignocellulosic materials[J]. Process Safety and Environmental Protection, 2012, 90（3）：189-202.
[14] Limayem A, Ricke SC. Lignocellulosic biomass for bioethanol production：current perspectives, potential issues and future prospects[J]. Progress in Energy and Combustion Science, 2012, 38（4）：449-467.
[15] Zhang K, Loretta J, Vara P, et al. Comparison of big bluestem with other native grasses：chemical composition and biofuel yield[J]. Energy, 2015, 83：358-365.
[16] Laura C, María BR, Mairan G, et al. Evaluation of dilute acid and alkaline pretreatments enzymatic hydrolysis and fermentation of napiergrass for fuel ethanol production[J]. Biomass and Bioenergy, 2015, 74：193-201.
[17] Kang KE, Chung DP, Kim Y, et al. High-titer ethanol production from simultaneous saccharification and fermentation using a continuous feeding system[J]. Fuel, 2015, 148：18-24.
[18] Wang Z, Lv Z, Du JL, et al. Combined process for ethanol fermentation at high-solids loading and biogas digestion from unwashed steam-exploded corn stover[J]. Bioresource Technology, 2014, 166：282-287.
[19] Camesasca L, María Belen R, Mairan G, et al. Evaluation of dilute acid and alkaline pretreatments, enzymatic hydrolysis and fermentation of napiergrass for fuel ethanol production[J]. ScienceDirect, 2015, 74：193- 201.
[20] Kallioinen A, Uusitalo J, Pahkala K, et al. Reed canary grass as a feedstock for 2nd generation bioethanol production[J]. Bioresource Technology, 2012, 123：669-672.
[21] Kaien R, Fumitaka S, Jun A, et al. Biomass yield and nitrogen use efficiency of cellulosic energy crops for ethanol production[J]. Biomass and Bioenergy, 2012, 37：330-334.
[22] Krzysztof JJ, Bogdan D, Wojciech SB, et al. Energy efficiency of crops grown for biogas production in a large-scale farm in Poland[J]. Energy, 2016, 109：277-286.
[23] Hamed M, Mashad E. Biomethane and ethanol production potential of spirulina platensis algae and enzymatically saccharified switchgrass[J]. Biochemical Engineering Journal, 2015, 93：119-127.
[24] Van Dyk JS, Pletschke BI. A review of lignocellulose bioconversion using enzymatic hydrolysis and synertistic cooperation between enzymes-factors affecing enzymes, conversion and synergy[J]. Biotechnol Adv, 2012, 30：1458-1480.
[25] Luis JG, Andrey E, Mariana P. King Grass：a promising material for the production of second-generation butanol[J]. Fuel, 2015, 4：399-403.
[26] Rashmi K, Rohit R, Ramesh B, et al. Saccharification of alkali treated biomass of kans grass contributes higher sugar in contrast to acid treated biomass[J]. Chemical Engineering Journal, 2013, 230：36-47.
[27] Suryawati L, Wilkins MR, Bellmer DD, et al. Effect of hydrothermolysis process conditions on pretreated switchgrass composition and ethanol yield by SSF with Kluyveromyces marxianus IMB4[J]. Process Biochemistry, 2009, 44（5）：540-545.
[28] Luca C, Samuele L, Carlo R, et al. Giant cane（Arundo donax L.）can substitute traditional energy crops in producing energy by anaerobic digestion, reducing surface area and costs：A full-scale approach[J]. Bioresource Technology, 2016, 218：826-832.
[29] Wahida R, Susanne FN, Veronica MH,et al. Methane production potential from Miscanthus sp.：Effect of harvesting time, genotypes and plant fractions[J]. Biosystems Engineering, 2015, 133：71-80.
[30] Kreuger E, Sipos B, Zacchi G, et al. Bioconversion of industrial hemp to ethanol and methane：The benefits of steam pretreatment and co-production[J]. Bioresource Technology, 2011, 3（102）：3457-3465.
[31] Simona M, Alexander Br, Franz T, et al. Biogas production from steam-exploded miscanthus and utilization of biogas energy and CO 2 in greenhouses[J]. Biomass and Bioenergy, 2013, 6：620-630.
[32] Castrillón L, Fernández NY, Ormaechea P, et al. Methane production from cattle manure supplemented with crude glycerin from the biodiesel industry in CSTR and IBR. [J]. Bioresource Technology 2013, 127：312-317.
[33] Wang F, Taira H, Jun T. Enhancement of anaerobic digestion of shredded grass by co-digestion with sewage sludge and hyperthermophilic pretreatment[J]. Bioresource Technology, 2014, 169：299-306.
[34] Dareioti MA, Kornaros M. Effect of hydraulic retention time（HRT）on the anaerobic co-digestion of agro-industrial wastes in a two-stage CSTR system[J]. Bioresource Technology, 2014：407-415.
[35] Kaparaju P, Serrano M, Angelidaki I. Optimization of biogas production from wheat straw stillage in UASB reactor[J]. Applied Energy, 2010, 87（12）：3779-3783.
[36] Lehtoma A, Nizami AS, Murphy JD, et al. Optimizing the thermophilic hydrolysis of grass silage in a two-phase anaerobic digestion system[J]. Bioresource Technology, 2013, 143：117-125.
[37] Carvalho AR, Fragosoa R, Gominho J. et al. Water-energy nexus：anaerobic co-digestion with elephant grass hydrolyzate[J]. Journal of Environmental Management, 2016, 181：48-53.
[38] Luo G. Anaerobic treatment of cassava stillage for hydrogen and methane production in continuously stirred tank reactor（CSTR）under high organic loading rate（OLR）[J]. Nrnaonal Jornal of Hydrogn Nrgy, 2010, （21）：11733-11737.
[39] Westerholm M, Hansson M, Schnürer A. Improved biogas production from whole stillage by co-digestion with cattle manure[J]. Bioresource Technology, 2012, 114：314-319.
[40] Zuo Z, Tian S, Chen ZB, et al. Soaking pretreatment of corn stover for bioethanolproduction followed by anaerobic digestion process[J]. Appl Biochem Biotechnol, 2012, 167：2088-2102.