金针菇Fv-Afe1基因的功能初探

doi:10.13560/j.cnki.biotech.bull.1985.2018-0019

生物技术通报 ›› 2018, Vol. 34 ›› Issue (3): 230-234.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0019

金针菇Fv-Afe1基因的功能初探

姜思源¹, 丑天胜¹, 李肖², 黄蓉梅¹, 谢宝贵¹

1. 福建农林大学菌物研究中心，福州 350002;
2. 中国科学院微生物研究所，北京 100080

收稿日期:2018-01-08 出版日期:2018-03-20 发布日期:2018-04-10
作者简介:姜思源，女，硕士研究生，研究方向食用菌分子机理；E-mail937581781@qq.com；丑天胜同为本文第一作者
基金资助:
国家重点基础研究发展计划（2014CB138302）

Preliminary Study on the Function of Fv-Afe1 Gene in Flammulina velutipes

JIANG Si-yuan¹, CHOU Tian-sheng¹, LI Xiao², HUANG Rong-mei¹, XIE Bao-gui¹

1. Mycological Research Center of Fujian Agricultural and Forestry University，Fuzhou 350002;
2. Institute of Microbiology，Chinese Academy of Sciences，Beijing 100080

Received:2018-01-08 Published:2018-03-20 Online:2018-04-10

摘要/Abstract

摘要： 从金针菇菌株L11基因组数据筛选出一个腺苷酸合成酶超家族基因Fv-Afe1。结合转录组数据分析基因结构，利用生物信息学软件对基因及其氨基酸序列生理生化指标进行预测，与NCBI已公布的其他物种的氨基酸序列进行系统发育分析，运用qRT-PCR技术和转录组表达谱数据对Fv-Afe1在金针菇各发育时期进行表达分析。结果显示，Fv-Afe1全长为967 bp，具有4个外显子和3个内含子，该基因氨基酸三级结构具有两个结构域，属于胞内酶，无跨膜结构，为真菌腺苷酸合成酶超家族成员；该基因在伸长期菌柄高表达，成熟期菌柄次之。

关键词: 金针菇, 子实体发育, 腺苷酸合成酶超家族

Abstract: According to genome data of a Flammulina velutipes strain L11，we selected Fv-Afe1 belonging to adenylate-forming enzymes superfamily. Transcriptome of F. velutipes was used to analyze gene structure，bioinformatics software to predict the amino acid sequence of Fv-Afe1，the predicted results and amino acid sequences of other species in NCBI were combined to construct phylogenetic tree，and qRT-PCR and DGE（digital gene expression profiling）data to analyze the gene expression in different growth stage. The results showed Fv-Afe1 gene contained 967 bp，including 4 exons and 3 introns. The results of bioinformatics revealed that the gene had two domains at tertiary structure of amino acids. Besides，the product of Fv-Afe1 was intracellular enzyme and no transmembrane structure，Fv-Afe1 belonging to the adenylate-forming enzymes superfamily was confirmed by analyzing phylogenetic tree，expression test showed that Fv-Afe1 had the highest expression in stipe-elongation stage and the secondly highest in stipe-maturation.

Key words: Flammulina velutipes, fruit body forming, adenylate-forming enzymes superfamily

姜思源, 丑天胜, 李肖, 黄蓉梅, 谢宝贵. 金针菇Fv-Afe1基因的功能初探[J]. 生物技术通报, 2018, 34(3): 230-234.

JIANG Si-yuan, CHOU Tian-sheng, LI Xiao, HUANG Rong-mei, XIE Bao-gui. Preliminary Study on the Function of Fv-Afe1 Gene in Flammulina velutipes[J]. Biotechnology Bulletin, 2018, 34(3): 230-234.

参考文献

［1］毕志树, 郑国扬, 李泰辉. 广东大型真菌志［M］. 广州：广东科技出版社, 1994.
［2］魏华, 谢俊杰. 金针菇营养保健作用［J］. 天然产物研究与开发, 1997, 9（2）：92-97.
［3］ Lu YP, Chen RL, Long Y, et al. A jacalin-related lectin regulated the formation of aerial mycelium and fruiting body in Flammulina velutipes［J］. Int J Mol Sci, 2016, 17（12）：1884.
［4］Zhang Z, Zhou R, Sauder JM, et al. Structural and functional studies of fatty Acyl adenylate ligases from E. coli and L. pneumophila［J］. J Mol Biol, 2011, 406：313-324.
［5］Bronstein I, Fortin J, Stanley PE, et al. Chemiluminescent and bioluminescent reporter gene assays［J］. Anal Biochem, 1994, 219：169-181.
［6］Greer LF III, Szalay AA. Imaging of light emission from the expression of luciferases in living cells and organisms：a review［J］. Luminescence, 2002, 17：43-73.
［7］Schmelz S, Naismith JH. Adenylate-forming enzymes［J］. Current Opinion in Structural Biology, 2009, 19：666-671.
［8］Wu R, Reger AS, Lu X, et al. The mechanism of domain alternation in the acyl-adenylate forming ligase superfamily member 4-chlorobenzoate：coenzyme A ligase［J］. Biochemistry, 2009, 48：4115-4125.
［9］Weimer KM, Shane BL, Brunetto M, et al. Evolutionary basis for the coupled-domain motions in Thermus thermophilus leucyl-tRNA synthetase［J］. J Biol Chem, 2009, 284（15）：10088-10099.
［10］ Perona JJ, Hou YM. Indirect readout of tRNA for aminoacylation;［J］. Biochemistry, 2007, 46：10419-10432.
［11］Inouye S. Firefly luciferase：an adenylate-forming enzyme for multicatalytic functions［J］. Cell Mol Life Sci, 2010, 67：387-404.
［12］Schulman BA, Harper JW. Ubiquitin-like protein activation by E1 enzymes：the apex for downstream signalling pathways［J］. Nat Rev Mol Cell Biol, 2009, 10：319-331.
［13］Shuman S. DNA ligases：progress and prospects［J］. J Biol Chem, 2009, 284：17365-17369.
［14］Chapman-Smith A, Cronan JJE. The enzymatic biotinylation of proteins：a post-translational modification of exceptional specificity［J］. Trends Biochem Sci, 1999, 24：359-363.
［15］Chen L, Petrelli R, Felczak K, et al. Pankiewicz, Nicotinamide adenine dinucleotide based therapeutics［J］. Curr Med Chem, 2008, 15：650-670.
［16］Watkins PA. Fatty acid activation, Prog［J］. Lipid Res, 1997, 36：55-83.
［17］Sieber SA, Marahiel MA. Molecular mechanisms underlying nonribosomal peptide synthesis：approaches to new antibiotics［J］. Chem Rev, 2005, 105：715-738.
［18］Gulick AM. Conformational dynamics in the acyl-CoA synthetases. Adenylation domains of non-ribosomal peptide synthetases, and firefly luciferase［J］. ACS Chem Biol, 2009, 4：811-827.
［19］吕作舟, 张引芳, 等. 食用菌关键技术问答：金针菇真姬菇杏鲍菇杨树菇［M］. 北京：化学工业出版社, 2010：42-64.
［20］Tamura K, Stecher G, Peterson D, et al. MEGA6：molecular evolutionary genetics analysis version 6. 0［J］. Molecular Biology and Evolution, 2013, 30（12）：2725-2729.
［21］严俊杰, 郭丽羡, 赵静静, 等. 草菇谷胱甘肽S-转移酶编码基因（vv-gto1）的序列分析及其差异表达［J］. 微生物学报, 2014, 10：1171-1177.
［22］Zhang ZF, Lin H, Ma B. ZOOM Lite：next-generation sequencing data mapping and visualization software［J］. Nucleic Acids Research, 2010, 38：743-748.
［23］李继影. 毛柄金线菌子实体菌柄细胞壁伸长生长的研究［D］. 南京：南京师范大学, 2005.
［24］Jacobson K, Mouritsen OG, Anderson RGW. Lipid rafts：at a crossroad between cell biology and physics［J］. Nat Cell Biol, 2007, 9（1）：7-14.
［25］Guan XL, Souza CM, Pichler H, et al. Functional interactions between sphingolipids and sterols in biological membranes regulating cell physiology［J］. Mol Biol Cell, 2009, 20（7）：2083-2095.
［26］Tyler KM, Fridberg A, Toriello KM, et al. Flagellar membrane localization via association with lipid rafts［J］. J Cell Sci, 2009, 122（6）：859-866.
［27］Lingwood D, Kai S. Lipid rafts as a membrane-organizing principle［J］. Science, 2010, 327（5961）：46-51.
［28］Rayle DL, Cleland R. Enhancement of wall loosening and elongation by acid solutions［J］. Plant Physiol, 1970, 46（2）：250-253.
［29］Robertson NF. Experimental control of hyphal branching and branch form in hyphomycetous fungi［J］. Journal of the Linnean Society of London, Botany, 1959, 56（366）：207-211.
［30］Thach TT, Luong TT, Lee S, et al. Adenylate kinase from Streptococcus pneumoniae is essential for growth through its catalytic activity［J］. FEBS Open Bio, 2014, 4：672-682.

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金针菇Fv-Afe1基因的功能初探

Preliminary Study on the Function of Fv-Afe1 Gene in Flammulina velutipes

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