白灵侧耳子实体发育相关基因的挖掘

doi:10.13560/j.cnki.biotech.bull.1985.2018-0139

生物技术通报 ›› 2018, Vol. 34 ›› Issue (4): 115-120.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0139

白灵侧耳子实体发育相关基因的挖掘

荣成博, 赵爽, 宋爽, 王晶, 刘宇

北京市农林科学院植物保护环境保护研究所北京市食用菌工程技术研究中心,北京 100097

收稿日期:2018-02-08 出版日期:2018-04-20 发布日期:2018-05-04
作者简介:荣成博,博士,助理研究员,研究方向：食用菌育种;E-mail：woshiboer@163.com
基金资助:
北京市农林科学院科技创新能力建设专项（KJCX20170106,KJCX20170205）,北京市农林科学院所级科技创新团队建设（JNKST201614）,北京市优秀人才培养资助计划（2015000020060G138）

Identification of Candidate Genes Related to the Development of Pleurotus tuoliensis Fruiting Bodies

RONG Cheng-bo, ZHAO Shuang, SONG Shuang, WANG Jing, LIU Yu

Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Science,Beijing Engineering Research Center for Edible Mushroom,Beijing 100097

Received:2018-02-08 Published:2018-04-20 Online:2018-05-04

摘要/Abstract

摘要： 白灵侧耳色泽洁白,营养丰富,且具有抗肿瘤、增强免疫力等多种功效,是我国具有自主知识产权的珍稀食用菌品种。探索白灵侧耳子实体发育相关基因能够为分子辅助育种提供有效、快速标记。实验前期获得了两株白灵侧耳双核菌株,JZB2106010能够结实形成子实体,JZB2106010的单核菌株经自交获得了1株不能形成子实体的双核菌株JZB2106103。以JZB2106010和JZB2106103为实验材料,进行转录组测序,获得了两个菌株的差异表达基因并进行了功能富集分析,其中催化活性和代谢过程本体中差异基因数目最多,选取部分表达差异显著的基因通过荧光定量PCR进行验证,结果显示编码苯丙氨酸裂解酶的基因（CL4509）和编码乙醇氧化酶的基因（CL2173）在JZB2106010中表达量显著提高,推断与白灵侧耳的子实体发育相关;另外还发现一个编码DNA解旋酶的基因（Unigene7793）在JZB2106103中的表达量是JZB2106010的1 046倍,推测该基因与白灵侧耳子实体发育呈负相关。

关键词: 白灵侧耳, 子实体发育, 转录组, 苯丙氨酸裂解酶, 乙醇氧化酶, DNA解旋酶

Abstract: Pleurotus tuoliensis contains rich nutrition and has many functions such as anti-tumor and enhancing immunity,etc.,thus it is precious edible white mushroom with independent intellectual property right in China. Identifying the candidate genes related to the formation of P. tuoliensis fruiting bodies would provide effective and rapid markers for its breeding. Two strains were obtained from the previous studies,one strain JZB2106010 formed fruiting bodies,while another dikaryotic hybrid strain JZB2106103 from protoplast monokaryons of JZB2106010 couldn’t form fruiting body. The transcriptome sequencing of JZB2106010 and JZB2106103 were performed to identify the differentially-expressed genes and functional enrichment analysis was carried out. GO enrichment displayed that there were the most differentially-expressed genes in catalytic activity and metabolic process. qPCR verification of partially the differentially-expressed genes showed that phenylalanine ammonium lyase（CL4509）and aryl-alcohol oxidase gene（CL2173）had significant high gene expressions in JZB2106010,revealing that CL4509 and CL2173 were related to the development of P. tuoliensis fruiting bodies. In addition,ATP dependent DNA helicase（Unigene7793）displayed 1 046-fold higher expression in JZB2106103 than in JZB2106010,inferring that Unigene7793 was negatively related to the development of P. tuoliensis fruiting bodies.

Key words: Pleurotus tuoliensis, development of fruiting bodies, transcriptome, phenylalanine ammonium lyase, aryl-alcohol oxidase, DNA helicase

荣成博, 赵爽, 宋爽, 王晶, 刘宇. 白灵侧耳子实体发育相关基因的挖掘[J]. 生物技术通报, 2018, 34(4): 115-120.

RONG Cheng-bo, ZHAO Shuang, SONG Shuang, WANG Jing, LIU Yu. Identification of Candidate Genes Related to the Development of Pleurotus tuoliensis Fruiting Bodies[J]. Biotechnology Bulletin, 2018, 34(4): 115-120.

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白灵侧耳子实体发育相关基因的挖掘

Identification of Candidate Genes Related to the Development of Pleurotus tuoliensis Fruiting Bodies

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