普通野生稻根特异启动子的克隆与鉴定

doi:10.13560/j.cnki.biotech.bull.1985.2018-0382

生物技术通报 ›› 2018, Vol. 34 ›› Issue (8): 87-92.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0382

普通野生稻根特异启动子的克隆与鉴定

黄珂¹, 黄格格¹, 薛满德², 龙艳², 袁潜华¹, 裴新梧²

1. 海南大学热带农林学院,海口 570228;
2. 农业部转基因安全评价重点实验室中国农业科学院生物技术研究所,北京 100081

收稿日期:2018-04-25 出版日期:2018-08-26 发布日期:2018-09-04
作者简介:黄珂,女,硕士,研究方向：作物基因工程;E-mail：huangke199309@163.com
基金资助:
转基因重大专项（2016ZX08011-001）,国家自然科学基金项目（31260153）

Cloning and Identification of a Promoter with Root Tissue-specific Expression in Common Wild Rice

HUANG Ke¹, HUANG Ge-ge¹, XUE Man-de², LONG Yan², YUAN Qian-hua¹, PEI Xin-wu²

1. College of Tropical Agriculture and Forestry,Hainan University,Haikou 570228;
2. MOA Key Laboratory on Safety Assessment（Molecular）of Agri-GMO,Chinese Academy of Agricultural Sciences,Beijing 100081

Received:2018-04-25 Published:2018-08-26 Online:2018-09-04

摘要/Abstract

摘要： 通过普通野生稻转录组文库筛选到一个普通野生稻（Oryza rufipogon Griff.）根特异表达基因,并克隆启动子序列,命名为OrRSGp,长度为896 bp,含有CAAT-box、TATA-box等主要功能元件,还有参与茉莉酸甲酯反应的响应元件,以及抗旱、抗逆等响应元件。将其与gus报告基因融合转化拟南芥,GUS组织化学染色与定量分析结果表明,OrRSGp调控gus基因在根部特异表达。

关键词: 普通野生稻, 启动子, 根特异表达

Abstract: Based on previous transcriptome data,a root-specific expression gene of common wild rice（Oryza rufipogon Griff.）was screened,and the promoter sequence was cloned and designated as OrRSGp. It was 896 bp in length and contained main functional elements such as CAAT-box and TATA-box,MeJA-responsiveness and some elements involved in defense and stress responsiveness. It was fused with the β-glucuronidas（gus）reporter gene and transformed into Arabidopsis thaliana. GUS histochemical staining and quantitative analysis showed that OrRSGp regulated gene gus specific expression in roots.

Key words: common wild rice, promoter, root tissue-specific expression

黄珂, 黄格格, 薛满德, 龙艳, 袁潜华, 裴新梧. 普通野生稻根特异启动子的克隆与鉴定[J]. 生物技术通报, 2018, 34(8): 87-92.

HUANG Ke, HUANG Ge-ge, XUE Man-de, LONG Yan, YUAN Qian-hua, PEI Xin-wu. Cloning and Identification of a Promoter with Root Tissue-specific Expression in Common Wild Rice[J]. Biotechnology Bulletin, 2018, 34(8): 87-92.

参考文献

[1] Li Y, Sun Y, Yang Q, et al.Cloning and function analysis of an alfalfa(Medicago sativa L.)zinc finger protein promoter MsZPP[J]. Mol Biol Rep, 2012, 39:855-8569.
[2] Odell JT, Nagy F,Chua NH. et. al. Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter[J]. Nature, 1985, 313:810-812.
[3] Leisner SM, Gelvin SB.Structure of the octopine synthase upstream activator sequence[J]. Proc Natl Acad Sci USA, 1988, 85:2552-2557.
[4] Mol JNM, Antoine RS, Alexander K.Genetic manipulation of floral pigmentation genes[J]. Plant Mol Biol, 1989, 13:287-294.
[5] McElroy D, Zhang W, et al. Isolation of an efficient actin promoter for use in rice transformation[J]. Plant Cell, 1990, 2:163-171.
[6] Christensen AH, Sharrock RA, Quail PH.Maize polyubiquitin genes:structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation[J]. Plant Mol Biol, 1992, 18:675-689.
[7] Fang RX, Nagy F, et al.Multiple cis-regulatory elements for maximal expression of the Cauliflower Mosaic Virus 35S promoter in transgenic plants[J]. Plant Cell, 1989, 1:141-150.
[8] Shelton AM, Zhao JZ, Roush RT.Economic, ecological, food safety, and social consequences of the deployment of bt transgenic plants[J]. Annu Rev Entomol, 2002, 47:845-881.
[9] Bhullar S, et al.Strategies for development of functionally equivalent promoters with minimum sequence homology for transgene expression in plants:cis-elements in a novel DNA context versus domain swapping[J]. Plant Physiol, 2003, 132:988-998.
[10] Charrier B, Scollan C, Ross S, et al.Co-silencing of homologous transgenes in tobacco[J]. Mol Breed, 2000, 6:407-419.
[11] Xu L, Ye R, Zheng Y, et al.Isolation of the endosperm specific LPAAT gene promoter from coconut(Cocos nucifera L.)and its functional analysis in transgenic rice plants[J]. Plant Cell Rep, 2010, 29:1061-1068.
[12] Kong, X, Zhang M, De Smet, et al. Designer crops:optimal root system architecture for nutrient acquisition[J]. Trends Biotechnol, 2014, 32:597-598.
[13] 周少霞. 江西东乡普通野生稻抗旱渗入系的构建及抗旱基因定位[D]. 北京:中国农业大学, 2005.
[14] Tian X, Long Y, Wang J, et al.De novo transcriptome assembly of common wild rice(O. rufipogon Griff.)and discovery of drought-response genes in root tissue based on transcriptomic data[J]. PLoS One, 2015, 10(7):e0131455.
[15] Jefferson RA, Kavanagh TA, Bevan MW.GUS fusions beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants[J]. EMBO J, 1987, 6(13):3901-3907.
[16] Banerjee J, Sahoo D, et al.A region containing an as-1 Element of Dahlia Mosaic Virus(DaMV)suhgenomic transcript promoter plays a key role in green tissue-and root-specific expression in plants[J]. Plant Mol Biol Reporter, 2015, 33(3):532-556.
[17] Price AH, Young EM, Tomos AD.Quantitative trait loci associated with stomatal conductance, leaf rolling and heading date mapped in upland rice(Oryza sativa)[J]. New Phytol, 1997, 137:83-91.
[18] Ohbayashi I, Sugiyama M.Plant nucleolar stress response, a new face in the NAC-dependent cellular stress responses[J]. Front Plant Sci, 2017, 8:2247.
[19] Jeong JS, Kim YS, et al.Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions[J]. Plant Physiol, 2010, 153(1):185-197.
[20] Yu Z, Kang B, et al.Root hair-specific expansins modulate root hair elongation in rice[J]. Plant J, 2011, 66(5):725-734.
[21] Chen L, Jiang B, Wu C, et al.GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots[J]. BMC Plant Biology, 2014, 14(1):245.
[22] Chen L, Jiang B, Wu C, et al.The characterization of GmTIP, a root-specific gene from soybean, and the expression analysis of its promoter[J]. Plant Cell, 2015, 121(2):259-274.
[23] Rausch C, Daram P, Brunner S, et al.A phosphate transporter expressed in arbuscule-containing cells in potato[J]. Nature, 2001, 414(6862):462-470.
[24] 胡标林, 余守武, 万勇, 等. 东乡普通野生稻全生育期抗旱性鉴定[J]. 作物学报, 2007, 33:425-432.
[25] Zhang X, Zhou S, Fu Y, et al.Identification of a drought tolerant introgression line derived from Dongxiang common wild rice(O. rufipogon Griff.). Plant Mol Biol, 2006, 62:247-59
[26] Zhang JW, Long Y, Xue MD, et al.Identification of microRNAs in Response to Drought in Common Wild Rice(O. rufipogon Griff.)Shoots and Roots[J]. PLoS One, 2017, 12(1):e0170330.
[27] 赵志强, 薛满德, 等. 普通野生稻绿色组织特异表达启动子的克隆与鉴定[J]. 生物技术通报, 2017, 33(8):51-57.

普通野生稻根特异启动子的克隆与鉴定

Cloning and Identification of a Promoter with Root Tissue-specific Expression in Common Wild Rice

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