生物技术通报 ›› 2019, Vol. 35 ›› Issue (5): 76-84.doi: 10.13560/j.cnki.biotech.bull.1985.2018-1098

• 研究报告 • 上一篇    下一篇

菌藻共生提高小球藻生物量和产油率

张靖洁, 段露露, 程蔚兰, 季春丽, 崔红利, 李润植   

  1. 山西农业大学分子农业与生物能源研究所,太谷030801
  • 收稿日期:2018-12-28 出版日期:2019-05-26 发布日期:2019-05-23
  • 作者简介:张靖洁,女,硕士研究生,研究方向:特色植物种质创制及利用;E-mail :1025858618@qq.com
  • 基金资助:
    国家自然科学基金项目(31401430),国家农业部“948”项目(2014-Z39),国家重点研发计划项目(2016YFE0106700),山西省煤基重点科技攻关项目(FT-2014-01),山西省重点科技项目(201603D312005),山西省留学归国人员科研基金项目(2015-064),中国博士后科学基金(2018M631768),辽宁省科学技术计划(自然科学基金指导)项目(20170540047),山西省重点研发计划一般项目(社会发展方面)(201803D31063),山西省应用基础研究(青年科技研究基金)项目(201801D22125)

Algae-bacteria Symbiosis Increases Biomass and Oil Production of Chlorella emersonii

ZHAGN Jing-jie, DUAN Lu-lu, CHENG Wei-lan, JI Chun-li, CUI Hong-li, LI Run-zhi   

  1. Institute of Molecular Agriculture and Bioenergy,Shanxi agriculture university,Taigu 030801
  • Received:2018-12-28 Published:2019-05-26 Online:2019-05-23

摘要: 微藻规模化养殖常伴随着细菌的影响,存在于微藻藻际的细菌对微藻生长的影响及藻菌共生的机理尚缺乏深入研究。为建立有益的菌藻共生体系和提高微藻生物质产量,以埃氏小球藻(Chlorella emersonii)为试材,分离藻际微环境的菌群,并运用16S rDNA测序进行鉴定。通过藻菌(1∶1)共培养筛选优势促生菌。人工构建不同比例的菌藻共培养体系,分析优势促生菌对微藻生长和生物质产量的影响。结果显示,从埃氏小球藻藻株SXND-25藻际分离到6个菌种,属于菠萝泛菌属(Pantoea)、假单胞菌属(Pseudomonas)、鹑鸡肠球菌属(Enterococcus gallinarum)和大肠杆菌属(Escherichia coli)四个菌属。其中假单胞菌(Pseudomonas)和菠萝泛菌(Pantoea)为优势促生菌。与其他不同比例菌藻共培养相比,埃氏小球藻与菠萝泛菌1∶5共培养的促生效果突出,埃氏小球藻在第8天生物量达5.86 g/L,藻细胞含油量为26.88%,总油脂产量为1.575 g/L且单不饱和脂肪酸(MUFA)高达554-564 mg/L。另一优异组合为埃氏小球藻与假单胞菌1∶1共培养,埃氏小球藻第8天生物量为4.12 g/ L,藻细胞含油量达29.50%,总油脂产量提高到1.215 g/L,但MUFA含量低(168-175 mg/L)。研究表明在埃氏小球藻培养过程中,适量添加促生菌,可同时提高埃氏小球藻生物质和油脂产量,这为探究藻菌互作效应以及有益藻菌共生体系应用于微藻规模化生产提供参考依据。

关键词: 埃氏小球藻(Chlorella emersonii), 藻际菌群, 藻菌共生, 微藻生物量, 油脂积累

Abstract: The large-scale cultivation of microalgae is often accompanied by bacteria and their infection. Limited information is available for the effect of bacteria in phycosphere on microalgae growth and the mechanism of algae-bacterial symbiosis. In order to establish a beneficial algae-bacterial symbiotic system and increase the biomass production of microalgae,Chlorella emersonii was selected as the test material,and microflora in phycosphere was isolated and then identified by 16S rDNA sequencing. The dominant growth-promoting bacteria were screened by co-culture of algae and bacteria(1∶1). Various algae-bacterial co-culture systems at different ratios were artificially constructed to analyze the effects of dominant growth-promoting bacteria on microalgae growth and biomass yield. The results showed that 6 bacteria were isolated from the phycosphere of C. emersonii strain SXND-25,belonging to the 4 genera Pantoea,Pseudomonas,Enterococcus gallinarum and Escherichia coli. Of them,Pseudomonas and Pantoea were the dominant growth-promoting bacteria. Compared with other co-culture systems at different ratios of algae and bacteria,a symbiotic system of C. emersonii and Pantoea(1∶5)showed outstanding growth-promoting effects. The biomass of C. emersonii increased to 5.86 g/L on the 8th day of cultivation,the oil content was 26.88% in algal cells,with 1.575 g/L total oil production and 554-564 mg/L monounsaturated fatty acid(MUFA). Another excellent combination was 1∶1 co-culture of C. emersonii and Pseudomonas. The biomass of C. emersonii on the 8th day was 4.12 g/L,the oil content of algal cells was 29.50%,and the total oil production reached 1.215 g/L,but low MUFA content(168-175 mg/L). The present study demonstrated that adding the appropriate amount of growth-promoting bacteria in the cultivation of C. emersonii simultaneously increased algal biomass and oil production,providing solid scientific reference for investigating the algal-bacterial interaction effect and the application of beneficial algal-bacterial symbiosis system in commercial production of microalgae.

Key words: Chlorella emersonii, bacterial flora/community in phycosphere, algae-bacteria symbiosis, algal biomass, oil accumulation.