斯达油脂酵母中自噬与油脂积累的关联性分析

doi:10.13560/j.cnki.biotech.bull.1985.2015.02.031

生物技术通报 ›› 2015, Vol. 31 ›› Issue (2): 208-216.doi: 10.13560/j.cnki.biotech.bull.1985.2015.02.031

斯达油脂酵母中自噬与油脂积累的关联性分析

韩冰, 杨琴, 崔清华

云南大学生命科学学院，昆明 650091

收稿日期:2014-07-14 出版日期:2015-02-05 发布日期:2015-02-06
作者简介:韩冰，男，硕士研究生，研究方向：自噬与油脂代谢关系的研究；E-mail：1017830953@qq.com
基金资助:
云南省创新团队基金项目（2011C1123），国家自然科学基金项目（31160237）

An Investigation of Cellular Autophagy and Oil Accumulation in the Lipomyces starkeyi

Han Bing, Yang Qin, Cui Qinghua

The School of Life Sciences，Yunnan University，Kunming 650091

Received:2014-07-14 Published:2015-02-05 Online:2015-02-06

摘要/Abstract

摘要： 旨在证明斯达油脂酵母中自噬参与油脂积累过程。在不同油脂积累水平下，检测相关自噬基因的表达，观察自噬与油脂积累是否有潜在相关性；用自噬促进剂促进自噬后，观察酵母油脂积累水平是否有差异。结果表明，与油脂低积累水平相比，在油脂高积累条件下，斯达油脂酵母自噬水平较低；用自噬促进剂处理后，酵母油脂积累降低。在斯达油脂酵母中，自噬与油脂积累成负相关性，自噬水平的提高会使酵母中油脂积累降低。

关键词: 自噬, 斯达油脂酵母, 油脂积累, 泛素样共轭体系

Abstract: It was to prove autophagy is involved in the accumulation of lipid in Lipomyces starkeyi. Detecting the expression of autophagy-related gene in different lipid accumulation level to determine whether autophagy is potentially involved in lipid accumulation; promoting autophagy by autophagy accelerator to observe whether there are differences in yeast lipid accumulation level. Results showed that the level of autophagy is low in high lipid accumulation condition compare with low condition. After treatment with autophagy accelerator, the lipid accumulation in yeast is reducing. In Lipomyces starkeyi, autophagy is negatively related to lipid accumulation, the improving of autophagy level will reduce the lipid accumulation in yeast.

Key words: autuphagy, Lipomyces starkeyi, oil accumulation, autophagy ubiquitin-like conjugated system

韩冰, 杨琴, 崔清华. 斯达油脂酵母中自噬与油脂积累的关联性分析[J]. 生物技术通报, 2015, 31(2): 208-216.

Han Bing, Yang Qin, Cui Qinghua. An Investigation of Cellular Autophagy and Oil Accumulation in the Lipomyces starkeyi[J]. Biotechnology Bulletin, 2015, 31(2): 208-216.

参考文献

[1]Reggiori F, Klionsky DJ. Autophagy in the eukaryotic cell[J]. Eukaryotic Cell, 2002, 1（1）：11-21.
[2]Klionsky DJ, Meijer AJ, Codogno P, et al. Views and commentaries autophagy and p70S6 kinase[J]. Autophagy, 2005, 1（1）：59-61.
[3] Tanida I, Ueno T, Kominami E. LC3 conjugation system in mamm-alian autophagy[J]. The International Journal of Biochemistry & Cell Biology, 2004, 36（12）：2503-2518.
[4] Kirisako T, Ichimura Y, Okada H, et al. The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway[J]. The Journal of Cell Biology, 2000, 151（2）：263-276.
[5] Cheong H, Nair U, Geng J, et al. The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesi- cle formation for nonspecific autophagy in Saccharomyces cerevisiae [J]. Molecular Biology of the Cell, 2008, 19（2）：668-681.
[6]Kihara A, Noda T, Ishihara N, et al. Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae[J]. The Journal of Cell Biology, 2001, 152（3）：519-530.
[7] Lapierre LR, Gelino S, Meléndez A, et al. Autophagy and lipid metabolism coordinately modulate life span in germline-less C. elegans[J]. Current Biology, 2011, 21（18）：1507-1514.
[8]Heaton NS, Randall G. Dengue virus-induced autophagy regulates lipid metabolism[J]. Cell Host & Microbe, 2010, 8（5）：422-432.
[9]Vescovo T, Romagnoli A, Perdomo AB, et al. Autophagy protects cells from HCV-induced defects in lipid metabolism[J]. Gastroenterology, 2012, 142（3）：644-653.
[10]Singh R, Kaushik S, Wang Y, et al. Autophagy regulates lipid metabolism[J]. Nature, 2009, 458（7242）：1131-1135.
[11]Zhu Z, Zhang S, Liu H, et al. A multi-omic map of the lipid-produ- cing yeast Rhodosporidium toruloides[J]. Nature Communica-tions, 2012, 3：1112.
[12]郭书贤, 王振伟, 刘欢, 等. 斯达油脂酵母发酵食品生产废水产油脂的研究[J]. 中国食品学报, 2010 （4）：245-252.
[13]Angerbauer C, Siebenhofer M, Mittelbach M, et al. Conversion of sewage sludge into lipids by Lipomyces starkeyi for biodiesel production[J]. Bioresource Technology, 2008, 99（8）：3051-3056.
[14]李仁民, 王菊芳, 马爽, 等.利用脂肪酸合成酶抑制剂和磷酸香草醛反应筛选高产油脂酵母菌[J]. 微生物学通报, 2008, 35（4）：545-549.
[15]Betts MJ, Guigó R, Agarwal P, et al. Exon structure conservation despite low sequence similarity：a relic of dramatic events in evolution?[J]. The EMBO Journal, 2001, 20（19）：5354-5360.
[16] Holdsworth JE, Veenhuis M, Ratledge C. Enzyme activities in oleaginous yeasts accumulating and utilizing exogenous or endogenous lipids[J]. Journal of General Microbiology, 1988, 134（11）：2907-2915.
[17] Komatsu M, Ueno T, Waguri S, et al. Constitutive autophagy：vital role in clearance of unfavorable proteins in neurons[J]. Cell Death & Differentiation, 2007, 14（5）：887-894.

斯达油脂酵母中自噬与油脂积累的关联性分析

An Investigation of Cellular Autophagy and Oil Accumulation in the Lipomyces starkeyi

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