核移植介导的哺乳动物体细胞核重编程研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2019-0168

摘要/Abstract

摘要： 哺乳动物的体细胞核移植技术已经发展了20年有余,重构胚发育过程中的核重编程异常是制约这项技术应用的主要障碍。目前,提高克隆效率的方法主要是通过调节重编程过程中的表观遗传修饰来修复重编程的错误,从而提高核移植胚胎的发育效率。综述了核移植后早期胚胎发育过程中供体核重编程的异常,讨论了修复这些异常表观遗传修饰的研究进展,并对可能影响核移植胚胎发育的重编程事件及新兴技术进行展望。

关键词: 体细胞核移植, 重编程, 表观遗传修饰

Abstract: Somatic cell nuclear transfer technology in mammalian has been developed for more than 20 years,however,the abnormal nuclear reprogramming during embryonic development is critical barrier to the application of this technology. At present,the key method to improve the efficiency of cloning is to overcome the error of reprogramming by adjusting the epigenetic modification in the reprogramming process,thereby improving the development efficiency of reconstructed embryos. Here,we summarize the abnormal of donor nuclear reprogramming in the development of early embryos after nuclear transfer,and discuss the research status in correcting these epigenetic modifications. Meanwhile,we prospect the possible reprogramming events and emerging technologies that influences the development of nuclear transfer embryos.

Key words: somatic cell nuclear transfer, reprogramming, epigenetic modification

吴霄, 庄站伟, 马晓莉, 黄思秀, 李紫聪, 徐铮. 核移植介导的哺乳动物体细胞核重编程研究进展[J]. 生物技术通报, 2019, 35(11): 187-194.

WU Xiao, ZHUANG Zhan-wei, MA Xiao-li, HUANG Si-xiu, LI Zi-cong, XU Zheng. Research Progress on the Nuclear Reprogramming After Somatic Cells Nuclear Transfer in Mammalian[J]. Biotechnology Bulletin, 2019, 35(11): 187-194.

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