关键词: 堆肥, 纤维素降解, 放线菌, 细菌, 纤维素酶活, 分离鉴定

Abstract: In order to solve the problem of slow degradation of straw in aerobic compost,strains that efficiently degrade cellulose were screened from corn straw compost. The cellulolytic bacteria in the corn stover compost were screened by the plate dilution method combined with the Congo red staining method,and the re-screening was conducted by the filter paper disintegration test. Two actinomycetes C31 and C37 with high filter paper disintegration ability were screened and identified as Streptomyces drozdowiczii and Streptomyces corchorusii. Strain GD16 was identified as Paenibacillus pabuli. At the same time,the enzyme activities of three strains were quantitatively measured. The strain C31 had the highest cellulase activity and filter paper enzyme activity,which were 4.8 U/mL and 3 U/mL,respectively. The strain GD16 had the highest xylanase activity,and reached 23 U/mL. The results showed that strain C31,C37 and GD16 all had cellulase activity,filter paper enzyme activity and xylanase activity.

Key words: compost, cellulose degradation, actinomycetes, bacteria, cellulase activity, isolation and identification