生物技术通报 ›› 2020, Vol. 36 ›› Issue (3): 177-182.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0916

• 研究报告 • 上一篇    下一篇

重组白介素2对红鳍东方鲀的血液生化指标及免疫指标的影响

保明秀, 藏林, 张红斌, 刘福君, 荆笛, 王秀利   

  1. 大连海洋大学水产与生命学院,大连 116023
  • 收稿日期:2019-09-28 出版日期:2020-03-26 发布日期:2020-03-17
  • 作者简介:保明秀,女,硕士研究生,研究方向:水产养殖动物基因工程;E-mail:mingxiubao@163.com
  • 基金资助:
    国家海水鱼产业技术体系岗位科学家项目(CARS-47),国家重点研发计划重点专项(2018YFD0900301-10)

Effects of Recombinant IL-2 on Blood Biochemical Parameters and Immune Indexes of Takifugu rubripes

BAO Ming-xiu, ZANG Lin, ZHANG Hong-bin, LIU Fu-jun, JING Di, WANG Xiu-li   

  1. College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023
  • Received:2019-09-28 Published:2020-03-26 Online:2020-03-17

摘要: 为研究重组白介素2(Recombinant interleukin-2,r IL-2)对红鳍东方鲀(Takifugu rubripes)血液生化指标及免疫指标的影响,选用体重为(205.9±30.5)g的红鳍东方鲀进行了重组白介素2的免疫应答实验。设计r IL-2免疫剂量为2.5、5、7.5、10 μg,并在免疫后的4 h、8 h和12 h进行采血。结果表明:经r IL-2免疫后红鳍东方鲀血清中尿素氮含量在各个浓度组的8 h时间点显著高于同组内其它时间点(P<0.05),4 h时间点甘油三酯含量有随着免疫浓度增加而增加的趋势,谷草转氨酶活性在8 h时间点要显著高于其他时间点(P<0.05),2.5 μg、5 μg浓度组过氧化氢酶的活性在4 h时间点显著高于其他各组(P<0.05),对免疫球蛋白的含量也有显著影响。本研究发现,r IL-2的免疫可引起血液生化指标和免疫指标的变化,尤其是超氧化物歧化酶和谷草转氨酶的变化较为显著(P<0.05),可在一定程度上促进红鳍东方鲀的免疫反应。

关键词: 红鳍东方鲀, 白细胞介素2, 血液生化指标

Abstract: In order to study the effects of recombinant interleukin-2(r IL-2)on blood biochemical parameters and immune indexes of Takifugu rubripes,the T. rubripes with a body weight of(205.9±30.5)g was selected. These T. rubripes were used for the immune response assay of recombinant interleukin 2.The r IL-2 immunization dose was designed to be 2.5,5,7.5,and 10 μg,and blood was collected at 4 h,8 h,and 12 h after immunization. The results showed that the content of urea nitrogen in T. rubripes serum after r IL-2 immunization was significantly higher than that in other groups at the 8h time point(P<0.05),and the triglyceride content at 4 h time point tended to increase with the rise of immunization dosage. The activity of aspartate aminotransferase at 8 h was higher than that at other time points(P<0.05). The activity of catalase in 2.5 and 5μg dosage group was significantly higher than that of other groups at 4 h(P<0.05). It also had a significant effect on the content of immunoglobulin. This study found that the injection of recombinant IL-2 caused changes in blood biochemical indicators and immune indexes,especially the changes of superoxide dismutase and aspartate aminotransferase(P<0.05),which may promote the immune response of T. rubripes to a certain extent.

Key words: Takifugu rubripes, interleukin-2, blood biochemical index