红鳍东方鲀转录因子GATA4基因的表达

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.022

摘要/Abstract

摘要： 利用荧光实时定量PCR(qRT-PCR)技术对红鳍东方鲀(Takifugu rubripes)成鱼的肾脏、肌肉、脑、脾脏、肝脏、精巢和卵巢等组织进行了GATA4基因的表达分析，并对幼鱼不同发育时间的个体水平上的GATA4基因的表达进行分析。结果显示，红鳍东方鲀成鱼的肌肉、脾脏、肾脏和脑等4个组织中的GATA4的表达量差异不显著，精巢、卵巢和肝脏的GATA4的表达量显著高于其他组织(P<0.05)，GATA4在精巢、肝脏中的表达量显著高于卵巢中(P<0.05)。在雄性幼鱼5个发育时期中(23、30、40、60和80日龄)，GATA4的表达量从23日龄到30日龄逐渐升高，30日龄最高且明显高于同时期雌鱼GATA4的表达量(P<0.01)，随后到40日龄之间表达量逐渐降低，从60日龄到80日龄又有上升的趋势。在雌性幼鱼5个发育时期中，GATA4表达量从23日龄到40日龄逐渐升高，40日龄最高且明显高于同时期雄鱼GATA4的表达量(P<0.01)，到60日龄期间表达量降低，从80日龄后又有上升趋势。各时期雌雄幼鱼，除40日龄外，其他各个时期在雄性中GATA4的表达量均高于在雌性中的表达量。

关键词: 红鳍东方鲀, 实时定量PCR, 转录因子GATA4, 基因表达

Abstract: In this study，GATA4 expression were analyzed in kidney，muscle，brain，spleen，liver，testis and ovary of mature Takifugu rubripes，and were analyzed at individual level during different times in young T. rubripes by flurescence quantitative Real-time Polymerase Chain Reaction(qRT-PCR). The results showed that no statistically significant difference was observed of expression level among muscle，spleen，kidney and brain. There was the significantly higher expressionof GATA4 in testis ，liver and ovary，than that in other organs(P<0.05). The GATA4 expression level of testis and liver were significantly higher(P<0.05)，than that in ovary. During the different periods(23，30，40，60，80 days of age(d)) of T. rubripes，the GATA4 expression level in males increased gradually from 23 d to 30 d and subsequently decreased at 40 d，and the 30 d expression level of GATA4 was higher than that in 30 d female individuals significantly(P<0.01). While from 60 d to 80 d，the GATA4 expression level went steadily up. In females，as the same as the expression tend in males，the GATA4 expression level increased gradually from 23 d to 40 d and subsequently decreased at 60 d，and the 40 d GATA4 gene expressed higher than that in the same time male individuals significantly(P<0.01). Nevertheless from 80 d，the GATA4 expression level stared to rise. Apart from 40 d individuals，the GATA4 expression level were higher in males than in female in every period.

Key words: Takifugu rubripes, quantitative Real-time PCR, transcription factor GATA4, gene expression

郝薇薇, 高长富, 仇雪梅, 姜志强, 王秀利,. 红鳍东方鲀转录因子GATA4基因的表达[J]. 生物技术通报, 2016, 32(6): 155-160.

HAO Wei-wei, GAO Chang-fu, QIU Xue-mei, JIANG Zhi-qiang, WANG Xiu-li. The Expression of Transcription Factor GATA4 Gene in Takifugu rubripes[J]. Biotechnology Bulletin, 2016, 32(6): 155-160.

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