杨树根际解无机磷细菌Mp1-Ha4的鉴定及其解磷机理

doi:10.13560/j.cnki.biotech.bull.1985.2019-0952

摘要/Abstract

摘要： 解无机磷细菌能够溶解土壤中的难溶性磷酸盐,增加土壤有效磷含量,促进植物生长。以一株杨树根际土壤中筛选得到的解无机磷细菌Mp1-Ha4为研究对象,利用分子生物学的方法对该菌株进行鉴定,测定了其对磷酸钙、磷酸铝和磷酸铁的解磷能力,并对该菌株9 d内的磷酸钙溶解动力学进行了研究。结果表明,解无机磷细菌Mp1-Ha4为一株西地西菌Cedecea sp.,其对磷酸钙的溶解能力远强于对磷酸铝和磷酸铁的溶解能力。在NBRIP液体培养基中,该菌株对磷酸钙的溶解能力达到了497.4 mg/L,在其对磷酸钙解磷过程中,培养基pH及可滴定酸度与解磷量分别呈显著负、正相关。高效液相色谱分析显示,该菌株在解磷过程中分泌了大量有机酸,主要包括α-酮戊二酸,酒石酸和苹果酸。分泌有机酸,降低环境pH可能是解无机磷细菌西地西菌（Cedecea sp.）Mp1-Ha4溶解难溶性磷酸盐的主要机制,同时该菌株对磷酸钙的高效溶解作用使其具有较大的研究和应用前景。

关键词: 解磷细菌, 磷酸钙, 有机酸

Abstract: Inorganic phosphate-solubilizing bacteria can dissolve insoluble phosphate in soil,increase available phosphorus content in soil and thus promote plant growth. An inorganic phosphate-solubilizing bacterium Mp1-Ha4 screened from poplar rhizosphere soil was used as the research object. The strain was identified by molecular biology method,and its phosphate-solubilizing ability to calcium phosphate,aluminum phosphate and iron phosphate was determined. The dissolution kinetics of calcium phosphate by this strain within 9 days was also studied. The results showed that the inorganic phosphate-solubilizing bacterium Mp1-Ha4 was a Cedecea sp.,and its ability to dissolve calcium phosphate was much stronger than that to dissolve aluminum phosphate and iron phosphate. In NBRIP liquid culture medium,the strain’s dissolving ability to calcium phosphate reached 497.4 mg/L. During the phosphate solubilization process,the pH and titratable acidity of the culture medium showed significant negative and positive correlations with the phosphate solubilization amount,respectively. High performance liquid chromatography analysis showed that the strain secreted a large amount of organic acids,mainly including α-ketoglutaric acid,tartaric acid and malic acid,in the process of phosphorus removal. Secreting organic acids and reducing environmental pH may be the main mechanism of dissolving insoluble phosphate by inorganic phosphate-solubilizing bacterium Mp1-Ha4. Meanwhile,the efficient dissolution of calcium phosphate by the strain has great research and application prospects.

Key words: phosphate-solubilizing bacteria, calcium phosphate, organic acid

韩雪娇, 曾庆伟, 赵玉萍. 杨树根际解无机磷细菌Mp1-Ha4的鉴定及其解磷机理[J]. 生物技术通报, 2020, 36(3): 141-147.

HAN Xue-jiao, ZENG Qing-wei, ZHAO Yu-ping. Identification of Inorganic Phosphate-solubilizing Bacterium Mp1-Ha4 in Poplar Rhizosphere and Its Phosphate-solubilizing Mechanism[J]. Biotechnology Bulletin, 2020, 36(3): 141-147.

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