苦参种子深加工过程油脂类副产物发酵产灵菌红素的研究

doi:10.13560/j.cnki.biotech.bull.1985.2019-1161

生物技术通报 ›› 2020, Vol. 36 ›› Issue (5): 130-138.doi: 10.13560/j.cnki.biotech.bull.1985.2019-1161

苦参种子深加工过程油脂类副产物发酵产灵菌红素的研究

倪亮^1,2, 张森¹, 郭盛¹, 曾飞¹, 徐明明¹, 段金廒¹

1.南京中医药大学江苏省中药资源产业化过程协同创新中心中药资源产业化与方剂创新药物国家地方联合工程研究中心国家中医药管理局中药资源循环利用重点研究室,南京 210023;
2.南京中医药大学附属医院,南京 210029

收稿日期:2019-11-29 出版日期:2020-05-26 发布日期:2020-06-03
作者简介:倪亮,男,硕士研究生,研究方向：中药资源循环利用;E-mail：niliang87@163.com
基金资助:
江苏省产学研合作前瞻性联合研究项目（BY2015008-04）,宁夏重点研发计划项目（2017BY079,2018ZWYQ0077）

Production of Prodigiosin Using Waste Oil from the Processing of Sophora flavescens Seeds

NI Liang^1,2, ZHANG Sen¹, GUO Sheng¹, ZENG Fei¹, XU Ming-ming¹, DUAN Jin-ao¹

1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine,Nanjing 210023;
2. The Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029

Received:2019-11-29 Published:2020-05-26 Online:2020-06-03

摘要/Abstract

摘要： 为提升苦参资源的利用效率,本研究以苦参种子提取生物碱过程中产生的副产物油脂类物质为研究对象,筛选可利用苦参种子废弃油脂生产灵菌红素的菌株并优化其发酵工艺。利用UPLC-Q-TOF-MS /MS对纯化后的发酵产物进行分析,并通过单因素考察和响应面优化获得菌株利用苦参种子油发酵产灵菌红素的最佳工艺参数。筛选到的菌株经形态和16S rDNA测序鉴定为粘质沙雷氏菌,并命名为粘质沙雷氏菌L9。优化的最佳发酵工艺条件为：苦参种子油、牛肉膏和氯化钙的最佳浓度分别为13 g/L、9.5 g/L及0.3 g/L,温度30℃;在最佳发酵工艺条件下,灵菌红素最高产量约为317.21 mg/L,产率提高约3.2倍。本研究以苦参种子深加工过程产生的副产物为研究对象,对其油脂类成分进行资源化利用研究,在有效处置苦参种子固废物的同时产生灵菌红素高附加值产品,为以种子类药材深加工过程固废物的资源化利用提供了借鉴。

关键词: 苦参种子, 粘质沙雷氏菌, 灵菌红素, 中药资源循环利用

Abstract: To improve the utilization efficiency of Sophora flavescens resources,the strains that can use the waste oil after extracting alkaloids from S. flavescens seed to produce prodigiosin were screened,and its fermentation parameters were optimized by single factor investigation and response surface optimization. UPLC-Q-TOF-MS/MS was used to analyze the purified fermented products. As the results,a strain L9 was screened and identified as Serratia marcescens by morphology and 16S rDNA sequencing. The optimal parameters were as follows：the optimal concentration of S. flavescens seed oil,beef extract and calcium chloride were 13 g/L,9.5 g/L and 0.3 g/L,respectively,and the fermentation temperature was 30℃. Under the optimal fermentation conditions,the maximum yield of prodigiosin was about 317.21 mg/L,and the yield was increased by 3.2 times. In this paper,the by-products produced in S. flavescens seed processing was taken as the research object,and its oil components were used as resources,i.e.,producing the high value-added product prodigiosin while disposing the wastes from processing S. flavescens seed,which provides a reference for the resource utilization of solid waste in the processing of seeds medicinal materials.

Key words: Sophora flavescens seed, Serratia marcescens, prodigiosin, recycling and utilization of Chinese medicine resources

倪亮, 张森, 郭盛, 曾飞, 徐明明, 段金廒. 苦参种子深加工过程油脂类副产物发酵产灵菌红素的研究[J]. 生物技术通报, 2020, 36(5): 130-138.

NI Liang, ZHANG Sen, GUO Sheng, ZENG Fei, XU Ming-ming, DUAN Jin-ao. Production of Prodigiosin Using Waste Oil from the Processing of Sophora flavescens Seeds[J]. Biotechnology Bulletin, 2020, 36(5): 130-138.

参考文献

[1] Stankovic N, Senerovic L, Ilic-Tomic T, et al.Properties and applications of undecylprodigiosin and other bacterial prodigiosins[J]. Applied Microbiology and Biotechnology, 2014, 98(9):3841-3858.
[2] Bennett JW, Bentley R.Seeing red:the story of prodigiosin[J]. Advances in Applied Microbiology, 2000, 47:1-32.
[3] Nikodinovicrunic J, Mojic M, Kang Y, et al.Undecylprodigiosin conjugated monodisperse gold nanoparticles efficiently cause apoptosis in colon cancer cells in vitro[J]. Journal of Materials Chemistry B, 2014, 2(21):3271-3281.
[4] Kim D, Kim JF, Yim JH, et al.Red to red- the marine bacterium Hahella chejuensis and its product prodigiosin for mitigation of harmful algal blooms[J]. Journal of Microbiology and Biotechnology, 2008, 18(10):1621-1629.
[5] Kumar K, Kumar V.Prodigiosin alkaloids:recent advancements in total synthesis and their biological potential[J]. Royal Society of Chemistry Advances, 2015, 5(15):10899-10920.
[6] Arivizhivendhan KV, Mahesh M, Boopathy R, et al.Antioxidant and antimicrobial activity of bioactive prodigiosin produces from Serratia marcescens using agricultural waste as a substrate[J]. Journal of Food Science and Technology, 2018. 55(7):2661-2670.
[7] Wang Z, Li B, Zhou L, et al.Prodigiosin inhibits Wnt/β-catenin signaling and exerts anticancer activity in breast cancer cells[J]. Proceedings of the National Academy of Sciences, 2017, 113(46):13150-13155.
[8] Goard CA, Schimmer A.An evidence-based review of obatoclax mesylate in the treatment of hematological malignancies[J]. Core Evidence, 2013, 8:15-26.
[9] Ahmad WA, Ahmad WYW, Zakaria ZA, et al.Application of bacterial pigments as colorant[M]. Springer Berlin Heidelberg, 2011, 57-74.
[10] Liu X, Wang Y, Sun S, et al.Mutant breeding of Serratia marcescens strain for enhancing prodigiosin production and application to textiles[J]. Preparative Biochemistry & Biotechnology, 2013, 43:271-284.
[11] Alihosseini F, Ju KS, et al.Antibacterial colorants:characteriz-ation of prodiginines and their applications on textile materials
[J]. Biotechnology Progress, 2010, 24(3):742-747.
[12] Alihosseini F, Lango J, Ju KS, et al.Mutation of bacterium Vibrio gazogenes for selective preparation of colorants[J]. Biotechnology Progress, 2010, 26(2):352-360.
[13] 马连杰, 张慧, 杭晓宁, 等. 一株产红色素细菌的分离鉴定及色素性质研究[J]. 天然产物研究与开发, 2018, 30(10):84-87.
[14] 刘晓侠, 沈振忠, 胡星夏, 等. 一株红色素高产菌的鉴定、发酵及结构表征[J]. 药物生物技术, 2010, 17(3):212-216.
[15] Giri AV, Anandkumar N, Muthukumaran G, et al.A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil[J]. BioMed Central Microbiology, 2004, 4(1):1-10.
[16] 翁泽斌, 段金廒, 郭盛, 等. 山西产苦参种子生物碱类化学成分分析及其资源化价值探讨[J]. 中国中药杂志, 2016, 41(17):3265-3271.
[17] 段金廒, 宿树兰, 郭盛, 等. 中药废弃物的转化增效资源化模式及其研究与实践[J]. 中国中药杂志, 2013, 38(23):3991-3996.
[18] Daoust JY, Gerber N.Isolation and purification of prodigiosin from Vibrio psychroerythrus[J]. Journal of Bacteriology, 1974, 118(2):756-757.
[19] 刘晓侠, 沈振忠, 胡星夏, 等. 一株红色素高产菌的鉴定、发酵及结构表征[J]. 药物生物技术, 2010, 17(3):212-216.
[20] Siva R, Subha K, Bhakta D, et al.Characterization and enhanced production of prodigiosin from the spoiled coconut[J]. Applied Biochemistry & Biotechnology, 2012, 166(1):187-196.
[21] Kurbanoglu EB, Ozdal M, Ozdal OG, et al.Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone[J]. Brazilian Journal of Microbiology, 2015, 46(2):631-637.
[22] Williamson NR, Fineran PC, Leeper FJ, et al.The biosynthesis and regulation of bacterial prodiginines[J]. Nature Reviews Microbiology, 2006, 4(12):887-899.
[23] Harris AKP, Williamson NR, Slater H, et al.The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation[J]. Microbiology, 2004, 150(11):3547-3560.
[24] Chen Y, Chang SKC, Chen J, et al.Characterization of microbial community succession during vermicomposting of medicinal herbal residues[J]. Bioresource Technology, 2018, 249:542-549.

苦参种子深加工过程油脂类副产物发酵产灵菌红素的研究

Production of Prodigiosin Using Waste Oil from the Processing of Sophora flavescens Seeds

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