生物技术通报 ›› 2020, Vol. 36 ›› Issue (5): 130-138.doi: 10.13560/j.cnki.biotech.bull.1985.2019-1161

• 研究报告 • 上一篇    下一篇

苦参种子深加工过程油脂类副产物发酵产灵菌红素的研究

倪亮1,2, 张森1, 郭盛1, 曾飞1, 徐明明1, 段金廒1   

  1. 1.南京中医药大学 江苏省中药资源产业化过程协同创新中心 中药资源产业化与方剂创新药物国家地方联合工程研究中心 国家中医药管理局中药资源循环利用重点研究室,南京 210023;
    2.南京中医药大学附属医院,南京 210029
  • 收稿日期:2019-11-29 出版日期:2020-05-26 发布日期:2020-06-03
  • 作者简介:倪亮,男,硕士研究生,研究方向:中药资源循环利用;E-mail:niliang87@163.com
  • 基金资助:
    江苏省产学研合作前瞻性联合研究项目(BY2015008-04),宁夏重点研发计划项目(2017BY079,2018ZWYQ0077)

Production of Prodigiosin Using Waste Oil from the Processing of Sophora flavescens Seeds

NI Liang1,2, ZHANG Sen1, GUO Sheng1, ZENG Fei1, XU Ming-ming1, DUAN Jin-ao1   

  1. 1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine,Nanjing 210023;
    2. The Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029
  • Received:2019-11-29 Published:2020-05-26 Online:2020-06-03

摘要: 为提升苦参资源的利用效率,本研究以苦参种子提取生物碱过程中产生的副产物油脂类物质为研究对象,筛选可利用苦参种子废弃油脂生产灵菌红素的菌株并优化其发酵工艺。利用UPLC-Q-TOF-MS /MS对纯化后的发酵产物进行分析,并通过单因素考察和响应面优化获得菌株利用苦参种子油发酵产灵菌红素的最佳工艺参数。筛选到的菌株经形态和16S rDNA测序鉴定为粘质沙雷氏菌,并命名为粘质沙雷氏菌L9。优化的最佳发酵工艺条件为:苦参种子油、牛肉膏和氯化钙的最佳浓度分别为13 g/L、9.5 g/L及0.3 g/L,温度30℃;在最佳发酵工艺条件下,灵菌红素最高产量约为317.21 mg/L,产率提高约3.2倍。本研究以苦参种子深加工过程产生的副产物为研究对象,对其油脂类成分进行资源化利用研究,在有效处置苦参种子固废物的同时产生灵菌红素高附加值产品,为以种子类药材深加工过程固废物的资源化利用提供了借鉴。

关键词: 苦参种子, 粘质沙雷氏菌, 灵菌红素, 中药资源循环利用

Abstract: To improve the utilization efficiency of Sophora flavescens resources,the strains that can use the waste oil after extracting alkaloids from S. flavescens seed to produce prodigiosin were screened,and its fermentation parameters were optimized by single factor investigation and response surface optimization. UPLC-Q-TOF-MS/MS was used to analyze the purified fermented products. As the results,a strain L9 was screened and identified as Serratia marcescens by morphology and 16S rDNA sequencing. The optimal parameters were as follows:the optimal concentration of S. flavescens seed oil,beef extract and calcium chloride were 13 g/L,9.5 g/L and 0.3 g/L,respectively,and the fermentation temperature was 30℃. Under the optimal fermentation conditions,the maximum yield of prodigiosin was about 317.21 mg/L,and the yield was increased by 3.2 times. In this paper,the by-products produced in S. flavescens seed processing was taken as the research object,and its oil components were used as resources,i.e.,producing the high value-added product prodigiosin while disposing the wastes from processing S. flavescens seed,which provides a reference for the resource utilization of solid waste in the processing of seeds medicinal materials.

Key words: Sophora flavescens seed, Serratia marcescens, prodigiosin, recycling and utilization of Chinese medicine resources