嗜热性木质纤维素酶在纤维素乙醇生产中的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2019-1267

摘要/Abstract

摘要： 聚焦于嗜热性木质纤维素酶在纤维素乙醇生产中的应用,归纳了限制纤维素乙醇商业化的技术瓶颈,简单介绍了嗜热性木质纤维素酶的特点,重点介绍了嗜热性木质纤维素酶在筛选、修饰、固定化、异源表达、代谢调控以及协同作用中的研究进展,讨论了嗜热性木质纤维素酶在纤维素乙醇生产中发挥的作用。最后,提出了嗜热性木质纤维素酶在纤维素乙醇生产中面临的问题并展望了其应用前景。

关键词: 纤维素乙醇, 嗜热性木质纤维素酶, 技术瓶颈, 生物燃料

Abstract: Focusing on the application of thermophilic lignocellulase in the production of cellulosic ethanol,the technical bottlenecks that limit the commercialization of cellulosic ethanol are summarized. Then the characteristics of thermophilic lignocellulase are briefly introduced,and the research advances on thermophilic lignocellulase in screening,modification,immobilization,heterologous expression,metabolic regulation and synergy are emphasized. Further,the role of thermophilic lignocellulase in the production of cellulosic ethanol is discussed. Finally,the problems while using thermophilic lignocellulase in the production of cellulosic ethanol are presented and its application is prospected.

Key words: cellulose ethanol, thermophilic lignocellulase, technical bottleneck, biofuel

刘登, 刘均洪. 嗜热性木质纤维素酶在纤维素乙醇生产中的研究进展[J]. 生物技术通报, 2020, 36(8): 185-193.

LIU Deng, LIU Jun-hong. Research Progress of Thermophilic Lignocellulase in Cellulose Ethanol Production[J]. Biotechnology Bulletin, 2020, 36(8): 185-193.

参考文献

[1] You KO, Hwang KR, Kim C, et al.Recent developments and key barriers to advanced biofuels:A short review[J]. Bioresource Technology, 2018, 257:320-333.
[2] 刘登, 刘均洪. 基因工程技术在生物燃料领域的应用进展[J]. 现代化工, 2019, 39(11):29-34.
Liu D, Liu JH.Progress in application of genetic engineering technology in biofuels field[J]. Modern Chemical Industry, 2019, 39(11):29-34.
[3] Akram F, Haq IU, Imran W, et al.Insight perspectives of thermostable endoglucanases for bioethanol production:A review[J]. Renewable Energy, 2018, 122:225-238.
[4] Kuhad RC, Deswal D, Sharma S, et al.Revisiting cellulase production and redefining current strategies based on major challenges[J]. Renewable and Sustainable Energy Reviews, 2016, 55:249-272.
[5] Elsayed M, Abomohra AF, Ai P, et al.Biorefining of rice straw by sequential fermentation and anaerobic digestion for bioethanol and/or biomethane production:Comparison of structural properties and energy output[J]. Bioresource Technology, 2018, 268:183-189.
[6] Bilal M, Hafiz MN, Hu HB, et al.Metabolic engineering and enzyme-mediated processing:A biotechnological venture towards biofuel production-A review[J]. Renewable and Sustainable Energy Reviews, 2018, 82(1):436-447.
[7] Donato PD, Ilaria Finore L, Poli A, et al.The production of second generation bioethanol:The biotechnology potential of thermophilic bacteria[J]. Journal of Cleaner Production, 2019, 233:1410-1417.
[8] Kaushal G, Kumar J, Sangwan RS, et al.Metagenomic analysis of geothermal water reservoir sites exploring carbohydrate-related thermozymes[J]. International Journal of Biological Macromolecules, 2018, 119:882-895.
[9] Ariaeenejad S, Maleki M, Hosseini E, et al.Mining of camel rumen metagenome to identify novel alkali-thermostable xylanase capable of enhancing the recalcitrant lignocellulosic biomass conversion[J]. Bioresource Technology, 2019, 281:343-350.
[10] Sindhu R, Binod P, Pandey A, Biological pretreatment of lignocellulosic biomass-An overview[J]. Bioresource Technology, 2016, 199:76-82.
[11] Patel AK, Singhania RR, Sim SJ, et al.Thermostable cellulases:Current status and perspectives[J]. Bioresource Technology, 2019, 279:385-392.
[12] Ebaid R, Wang HC, Sha C, et al.Recent trends in hyperthermophilic enzymes production and future perspectives for biofuel industry:A critical review[J]. Journal of Cleaner Production, 2019, 238:117925.
[13] Stevens JC, Shi J.Biocatalysis in ionic liquids for lignin valorization:Opportunities and recent developments[J]. Biotechnology Advances, 2019:37(8):107418.
[14] Han HW, Ling ZM, Khan A, et al.Improvements of thermophilic enzymes:From genetic modifications to applications[J]. Bioresource Technology, 2019, 279:350-361.
[15] Veluchamy C, Kalamdhad AS.Enhancement of hydrolysis of lignocellulose waste pulp and paper mill sludge through different heating processes on thermal pretreatment[J]. Journal of Cleaner Production, 2017, 168:219-226.
[16] Saurabh S, Kumar JD, Nallusamy S, et al.Developing efficient thermophilic cellulose degrading consortium for glucose production from different agro-residues[J]. Frontiers in Energy Research, 2019, 7:61.
[17] Aikawa S, Baramee S, Sermsathanaswadi J, et al.Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium[J]. Systematic and Applied Microbiology, 2018, 41(4):261-269.
[18] Tan H, Miao R, Liu T, et al.A bifunctional cellulase-xylanase of a new Chryseobacterium strain isolated from the dung of a straw-fed cattle[J]. Microbial Biotechnology, 2018, 11(2):381-398.
[19] Shahi S, Tajwar R, Akhtar MW, A novel trifunctional, family GH10 enzyme from Acidothermus cellulolyticus 11B, exhibiting endo-xylanase, arabinofuranosidase and acetyl xylan esterase activities[J]. Extremophiles, 2018, 22:109-119.
[20] Ariaeenejad S, Maleki M, Hosseini E, et al.Mining of camel rumen metagenome to identify novel alkali-thermostable xylanase capable of enhancing the recalcitrant lignocellulosic biomass conversion[J]. Bioresource Technology, 2019, 281:343-350.
[21] Krüger A, Schäfers C, Schröder C, et al.Towards a sustainable biobased industry - Highlighting the impact of extremophiles[J]. New Biotechnology, 2018, 40(A):144-153.
[22] Brogan APS, Le LB, Hallett JP.Non-aqueous homogenous biocatalytic conversion of polysaccharides in ionic liquids using chemically modified glucosidase[J]. Nature Chemistry, 2018, 10:859-865.
[23] Darby JF, Atobe M, Firth JD, et al.Increase of enzyme activity through specific covalent modification with fragments[J]. Chemical Science, 2017, 8(11):7772-7779.
[24] Boonyapakron K, Jaruwat A, Liwnaree B, et al.Structure-based protein engineering for thermostable and alkaliphilic enhancement of endo-β-1, 4-xylanase for applications in pulp bleaching[J]. Journal of Biotechnology, 2017, 259:95-102.
[25] Tajwar R, Shahid S, Zafar R, et al.Impact of orientation of carbohydrate binding modules family 22 and 6 on the catalytic activity of Thermotoga maritima xylanase XynB[J]. Enzyme and Microbial Technology, 2017, 106:75-82.
[26] Fernández-Fernández M, Moldes D, Domínguez A, et al.Stability and kinetic behavior of immobilized laccase from Myceliophthora thermophila in the presence of the ionic liquid 1-ethyl-3-methylimidazolium ethylsulfate[J]. Biotechnolgy Progress, 2014, 30(4):790-796.
[27] Gupta SMN, Bisaria VS.Effectiveness of cross-linked enzyme aggregates of cellulolytic enzymes in hydrolyzing wheat straw[J]. Journal of Bioscience and Bioengineering, 2018, 126(4):445-450.
[28] Periyasamy K, Santhalembi L, Mortha G, et al.Carrier-free co-immobilization of xylanase, cellulase and β-1, 3-glucanase as combined cross-linked enzyme aggregates(combi-CLEAs)for one-pot saccharification of sugarcane bagasse[J]. RSC Advances, 2016, 6:32849-32857.
[29] Jia J, Zhang W, Yang Z, et al.Novel magnetic cross-linked cellulase aggregates with a potential application in lignocellulosic biomass bioconversion[J]. Molecules, 2017, 22:269.
[30] Benedetti M, Vecchi V, Betterle N, et al.Design of a highly thermostable hemicellulose-degrading blend from Thermotoga neapolitana for the treatment of lignocellulosic biomass[J]. Journal of Biotechnology, 2019, 296:42-52.
[31] Haq LU, Akram F.Insight into kinetics and thermodynamics of a novel hyperstable GH family 10 endo-1, 4-β-xylanase(TnXynB)with broad substrates specificity cloned from Thermotoga naphthophilaRKU-10T[J]. Enzyme and Microbial Technology, 2019, 127:32-42.
[32] Han C, Li WG, Hua CY, et al.Enhancement of catalytic activity and thermostability of a thermostable cellobiohydrolase from Chaetomium thermophilum by site-directed mutagenesis[J]. International Journal of Biological Macromolecules, 2018, 116:691-697.
[33] Mir BA, Mewalal R, Mizrachi E, et al.Recombinant hyperthermophilic enzyme expression in plants:a novel approach for lignocellulose digestion[J]. Trends in Biotechnology, 2014, 32(5):281-289.
[34] Mir BA, Myburg AA, Mizrachi E, et al.In planta expression of hyperthermophilic enzymes as a strategy for accelerated lignocellulosic digestion[J]. Scientific Reports, 2017, 7(1):11462.
[35] Castiglia D, Sannino L, Marcolongo L, et al.High-level expression of thermostable cellulolytic enzymes in tobacco transplastomic plants and their use in hydrolysis of an industrially pretreated Arundo donax L. biomass[J]. Biotechnology for Biofuels, 2016, 9(1):154.
[36] Xiao Y, He X, Ojeda-Lassalle Y, et al.Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility[J]. Biotechnol Biofuels, 2018, 11:225.
[37] Kubicek CP.Systems biological approaches towards understanding cellulase production by Trichoderma reesei[J]. Journal of Biotechnology, 2013, 163(2):133-142.
[38] Aghcheh RK, Németh Z, Atanasova L, et al.The VELVET A orthologue VEL1 of Trichoderma reesei regulates fungal development and is essential for cellulase gene expression[J]. PLoS One, 2014, 9(11):e112799.
[39] Zhang F, Zhao XQ, Bai FW.Improvement of cellulase production in Trichoderma reesei Rut-C30 by overexpression of a novel regulatory gene Trvib-1[J]. Bioresource Technology, 2018, 247:676-683.
[40] Sun Y, Wang H, Ma K, et al.Construction and characterization of the GFAT gene as a novel selection marker in Aspergillus nidulans[J]. Applied Microbiology and Biotechnology, 2018, 102(18):7951-7962.
[41] Jain KK, Kumar A, Shankar A, et al.De novo transcriptome assembly and protein profiling of copper-induced lignocellulolytic fungus Ganoderma lucidum MDU-7 reveals genes involved in lignocellulose degradation and terpenoid biosynthetic pathways[J]. Genomics, 2020, 112(1):184-198.
[42] Han HW, Ling ZM, Khan A, et al.Improvements of thermophilic enzymes:From genetic modifications to applications[J]. Bioresource Technology, 2019, 279:350-361.
[43] Usai G, Cirrincione S, Re A, et al.Clostridium cellulovorans metabolism of cellulose as studied by comparative proteomic approach[J]. Journal of Proteomics, 2020, 216:103667.
[44] Aulitto M, Fusco FA, Fiorentino G, et al.A thermophilic enzymatic cocktail for galactomannans degradation[J]. Enzyme and Microbial Technology, 2018, 111:7-11.
[45] Zhou HC, Li T, Yu ZC, et al.A lytic polysaccharide monooxygenase from Myceliophthora thermophila and its synergism with cellobiohydrolases in cellulose hydrolysis[J]. International Journal of Biological Macromolecules, 2019, 139:570-576.
[46] Zhuo R, Yu HB, Qin X, et al.Heterologous expression and characterization of a xylanase and xylosidase from white rot fungi and their application in synergistic hydrolysis of lignocellulose[J]. Chemosphere, 2018, 212:24-33.
[47] Ding DY, Li PY, Zhang XM, et al.Synergy of hemicelluloses removal and bovine serum albumin blocking of lignin for enhanced enzymatic hydrolysis[J]. Bioresource Technology, 2019, 273:231-236.