结缕草响应立枯丝核菌侵染的SA合成途径鉴定

doi:10.13560/j.cnki.biotech.bull.1985.2020-0103

摘要/Abstract

摘要：

为揭示立枯丝核菌（Rhizoctonia solani）侵染条件下结缕草（Zoysia japonica）中水杨酸（SA）合成的主要途径,通过同源比对法识别关键调控基因,依据病程下的转录模式鉴定响应通路,测试SA浓度及苯丙氨酸解氨酶（PAL）活性验证表达效果。ZjPAL1-ZjPAL5作为典型PAL等位基因,对应Unigene于根部发病后呈现持续上调,在转录水平积极响应侵染。异分支酸合成酶基因中仅监测到ZjICS1表达,且丰度始终不足ZjPAL1-ZjPAL5的10％,并随侵染加深趋于下调。菌丝体侵入根部内皮层后引起PAL活性严重减弱,伴随其减弱程度加剧,途径终产物SA浓度的下降也加快;但同时在尚未感染的叶片中SA浓度却随PAL活性提升保持高水平。PAL基因调控的苯丙氨酸途径是R. solani侵染下结缕草SA合成主导途径,响应强度随侵染深入而增强,PAL活性下降会显著降低SA水平。

关键词: 结缕草, 立枯丝核菌, 水杨酸合成, 苯丙氨酸途径

Abstract:

To reveal the main salicylic acid（SA）biosynthetic pathway in Rhizoctonia solani-infected Zoysia japonica,the key genes was recognized with aligned homologous sequences,the responsive pathway was identified according to transcriptional profile during pathogenesis,SA concentration was measured,and phenylalanine ammonia lyase（PAL）activity was verified. As typical alleles of PAL,genes ZjPAL1-ZjPAL5 were up-regulated continuously in the diseased root shown by their corresponding Unigenes,meaning an active response to infection at transcription level. About genes encoding isochorismate synthase,only ZjICS1 transcripts were observed,whose abundance was always less than one-tenth of that of ZjPAL1-ZjPAL5 and tended downwards with infection increasing. The PAL activity in the root reduced after mycelial invasion into the endodermis of the root. The more the activity was weakening,the faster the decrease of synthesized SA concentration was. However,the SA concentration in the non-infected leaves remained high by enhanced PAL activity. Phenylalanine pathway regulated by PAL gene plays a major role in SA synthesis when Z. japonica is infected by R. solani. Responsive intensity increases with the infection deepening,and the reduction of PAL activity will result in SA concentration decreasing.

Key words: Zoysia japonica, Rhizoctonia solani, salicylic acid synthesis, phenylalanine pathway

宿强, 左慧, 晁跃辉, 曾会明. 结缕草响应立枯丝核菌侵染的SA合成途径鉴定[J]. 生物技术通报, 2020, 36(10): 32-39.

XU Qiang, ZUO Hui, CHAO Yue-hui, ZENG Hui-ming. Identification of SA Synthetic Pathways Responding to Rhizoctonia solani Infection in Zoysia japonica[J]. Biotechnology Bulletin, 2020, 36(10): 32-39.

图/表 6

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索引基因及关联家族	结缕草基因座基因座编号	基因名称
AtPAL1（AT2G37040）AtPAL2（AT3G53260） AtPAL3（AT5G04230）AtPAL4（AT3G10340） HOM04D000680家族含276条双子叶植物序列 HOM04M000387家族含212条单子叶植物序列	Zjn_sc00017.1.g00060.1.sm.mkhc Zjn_sc00007.1.g11910.1.sm.mkhc Zjn_sc00004.1.g04560.1.sm.mkhc Zjn_sc00017.1.g00050.1.sm.mkhc Zjn_sc00004.1.g04570.1.sm.mkhc Zjn_sc00103.1.g00580.1.am.mk Zjn_sc00007.1.g11880.1.am.mk Zjn_sc00007.1.g11890.1.sm.mk Zjn_sc06393.1.g00010.1.am.mk	ZjPAL1 ZjPAL2 ZjPAL3 ZjPAL4 ZjPAL5 ZjPAL6 ZjPAL7 ZjPAL8 ZjPAL9
AtICS1（AT1G74710）AtICS2（AT1G18870） HOM04D003911家族含57条双子叶植物序列 HOM04M000507家族含18条单子叶植物序列	Zjn_sc00039.1.g05120.1.sm.mkhc Zjn_sc00018.1.g05620.1.am.mkhc Zjn_sc00039.1.g05130.1.sm.mkhc Zjn_sc00018.1.g05610.1.sm.mkhc	ZjICS1 ZjICS2 ZjICS3 ZjICS4

索引基因及关联家族	结缕草基因座基因座编号	基因名称
AtPAL1（AT2G37040）AtPAL2（AT3G53260） AtPAL3（AT5G04230）AtPAL4（AT3G10340） HOM04D000680家族含276条双子叶植物序列 HOM04M000387家族含212条单子叶植物序列	Zjn_sc00017.1.g00060.1.sm.mkhc Zjn_sc00007.1.g11910.1.sm.mkhc Zjn_sc00004.1.g04560.1.sm.mkhc Zjn_sc00017.1.g00050.1.sm.mkhc Zjn_sc00004.1.g04570.1.sm.mkhc Zjn_sc00103.1.g00580.1.am.mk Zjn_sc00007.1.g11880.1.am.mk Zjn_sc00007.1.g11890.1.sm.mk Zjn_sc06393.1.g00010.1.am.mk	ZjPAL1 ZjPAL2 ZjPAL3 ZjPAL4 ZjPAL5 ZjPAL6 ZjPAL7 ZjPAL8 ZjPAL9
AtICS1（AT1G74710）AtICS2（AT1G18870） HOM04D003911家族含57条双子叶植物序列 HOM04M000507家族含18条单子叶植物序列	Zjn_sc00039.1.g05120.1.sm.mkhc Zjn_sc00018.1.g05620.1.am.mkhc Zjn_sc00039.1.g05130.1.sm.mkhc Zjn_sc00018.1.g05610.1.sm.mkhc	ZjICS1 ZjICS2 ZjICS3 ZjICS4