生物技术通报 ›› 2020, Vol. 36 ›› Issue (10): 25-31.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0092

• 研究报告 • 上一篇    下一篇

大豆疫病拮抗菌的筛选及促生抗病作用研究

张鸿雁1(), 高擎2, 张琳园2, 林国莉1, 李如莲1   

  1. 1.岭南师范学院生命科学与技术学院,湛江 524048
    2.黑龙江八一农垦大学生命科学技术学院,大庆 163319
  • 收稿日期:2020-02-01 出版日期:2020-10-26 发布日期:2020-11-02
  • 作者简介:张鸿雁,女,博士,教授,研究方向:资源与环境微生物;E-mail: zhy2219@163.com
  • 基金资助:
    岭南师范学院人才专项(ZL2035)

Screening of Actinomycetes Against Phytophthora Root Rot of Soybean and Its Growth Promotion and Disease Control

ZHANG Hong-yan1(), GAO qing2, ZHANG Lin-yuan2, LIN Guo-li1, LI Ru-lian1   

  1. 1. College of Life Science and Technology,Lingnan Normal University,Zhanjiang 524048
    2. College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319
  • Received:2020-02-01 Published:2020-10-26 Online:2020-11-02

摘要:

为筛选对大豆疫病有拮抗作用的菌株,以疫病病原菌大豆疫霉Phytophthora sojae为供试病原菌,以大豆根际微生物为材料,采用琼脂块法和生长速率法进行拮抗菌分离,获得了对病原菌有明显拮抗效果的放线菌XS1-5。经形态学指标、理化指标和分子生物学指标鉴定为白孢链霉菌(Streptomyces albosporeus)。利用不同浓度拮抗菌XS1-5孢子进行大豆种子包衣试验,结果表明,拮抗菌XS1-5促进大豆生长,提高叶片叶绿素含量、根系活力,增加叶片和根系保护性酶活性。XS1-5孢子浓度为1.0×108 CFU/g包衣后,大豆株高、根长、地上鲜重和地下鲜重分别增加19.92%、28.26%、87.40%和34.51%,叶绿素a(Ca)含量(7.53 mg/L)比对照增加50.6%,根系活力(685.41 μg/g·h)为对照的2.4倍。同时大豆叶片CAT、SOD和POD活力分别增加158.86%、459.71%和135.45%。本研究筛选的拮抗菌XS1-5对大豆有一定的促生抗病作用。

关键词: 大豆疫病, 放线菌, 促生, 抗病

Abstract:

To screen the strain with good antagonistic effect against the phytophthora root rot of soybean,actinomycete XS1-5 with significant antagonism was isolated from soybean rhizosphere soil by agar block method and mycelia growth assay while taking the Phytophthora sojae of phytophthora root rot pathogen. Strain XS1-5 was identified as Streptomyces albosporeus based on morphological,physiochemical characters as well as molecular methods. The pot experiment was carried out with soybean seeds coated with different spore concentrations of the XS1-5. The results showed the application of the XS1-5 significantly promoted soybean growth,and increased chlorophyll content,root vitality and the protective enzymes in the leaf and root of soybean. When spore concentration was 1.0×10 8 CFU/g,the plant height,root length,fresh weight and dry weight increased by 19.92%、28.26%、87.40% and 34.51%;meanwhile,chlorophyll content(7.53 mg/L)by 50.6% and root vitality(685.41 μg/g·h)by 2.4 folds increased,compared with the control,respectively,as well as the CAT,SOD and POD activity of leaf increased significantly by 158.86%,459.71% and 135.45%. The above results indicate that the antagonistic strain XS1-5 screened in this study has a certain disease-control and growth-promoting effect on soybean.

Key words: phytophthora root rot, actinomycetes, growth promotion, disease control