生物技术通报 ›› 2021, Vol. 37 ›› Issue (8): 294-306.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1314
严慧(), 胡文悦, 宋晓晴, 纪守坤, 刘萱, 黄卓涵, 刘月琴, 张英杰()
收稿日期:
2020-10-25
出版日期:
2021-08-26
发布日期:
2021-09-10
作者简介:
严慧,女,博士,副教授,研究方向:瘤胃菌种分离和微生态制剂开发;E-mail: 基金资助:
YAN Hui(), HU Wen-yue, SONG Xiao-qing, JI Shou-kun, LIU Xuan, HUANG Zhuo-han, LIU Yue-qin, ZHANG Ying-jie()
Received:
2020-10-25
Published:
2021-08-26
Online:
2021-09-10
摘要:
瘤胃微生物的发酵使反刍动物能够有效利用粗纤维等营养物质,瘤胃作为自然界的天然饲料发酵罐,是微生态菌剂开发的重要来源,包括纤维素、木聚糖、脂肪、毒素降解菌、非蛋白氮利用菌、甲烷氧化菌等。然而,瘤胃中大部分的微生物仍不可被培养,人工培养方法和流程的参差不齐限制了瘤胃菌种资源的开发利用。因此,本文基于国内外已有的瘤胃细菌分离研究进展,从分离标准步骤、现有研究现状和改进意见三方面做了综述,旨在为瘤胃功能细菌的分离提供更为全面的技术参考和现状概述。
严慧, 胡文悦, 宋晓晴, 纪守坤, 刘萱, 黄卓涵, 刘月琴, 张英杰. 瘤胃细菌的分离培养研究进展[J]. 生物技术通报, 2021, 37(8): 294-306.
YAN Hui, HU Wen-yue, SONG Xiao-qing, JI Shou-kun, LIU Xuan, HUANG Zhuo-han, LIU Yue-qin, ZHANG Ying-jie. Research Advances in Isolation and Cultivation of Rumen Bacteria[J]. Biotechnology Bulletin, 2021, 37(8): 294-306.
培养基类型 Types of culture medium | 分离菌株 Isolated strains | 参考文献 References |
---|---|---|
CMC-Na分离培养基 | 芽孢杆菌属、蜡样芽孢杆菌、木聚糖降解球形芽孢杆菌、枯草芽孢杆菌、环状芽孢杆菌、苏云金芽孢杆菌、巨大芽孢杆菌、短小芽孢杆菌、短杆菌、假单胞菌属、铜绿假单胞菌、香茅醇假单胞菌、寡养单胞菌属、纤维单胞菌属、肠杆菌属、棒状杆菌属、微杆菌属、苍白杆菌属、大肠杆菌、鞘氨醇杆菌属、肠球菌属、粪肠球菌、副球菌属、黄色瘤胃球菌、白色瘤胃球菌、链球菌、菠萝泛菌、肺炎克雷伯菌、产酸克雷伯氏菌、溶纤维丁酸弧菌、拜氏羧菌、丁酸梭菌 | [ |
木聚糖唯一碳源培养基 | 溶纤维丁酸弧菌、类芽孢杆菌属 | [ |
酪蛋白主要氮源培养基 | 嗜淀粉拟杆菌、丁酸弧菌属、新月形单胞菌属、毛螺旋菌属、未知种 | [ |
双缩脲、尿素唯一氮源培养基 | 葡萄球菌属、干酪乳杆菌、产气克雷伯氏菌、粪链球菌 | [ |
无氮培养基 | 克雷伯氏菌属、弗兰克氏菌属、芽孢杆菌属 | [ |
LSB培养基 | 痤疮丙酸杆菌、蜡样芽孢杆菌、草乳杆菌、弯曲乳杆菌、吉氏库特菌、粪肠球菌、解淀粉芽孢杆菌、牛链球菌、志贺氏菌、氧化木糖无色杆菌、夏威夷肠球菌、产粘棒状杆菌 | [ |
CDC厌氧血琼脂 | 反刍月形单胞菌、粪肠球菌 | [ |
MRS培养基 | 巴黎链球菌、肠膜明串珠菌、戊糖片球菌、弯曲乳杆菌、耐久肠球菌、海氏肠球菌、鹑鸡肠球菌、屎肠球菌、粪肠球菌 | [ |
SL培养基 | 干酪乳杆菌,黏膜乳杆菌 | [ |
厌氧杆菌培养基 | 产琥珀酸放线杆菌 | [ |
牛肉膏蛋白胨溴甲酚绿培养基 | 放线杆菌属、肠膜明串珠菌 | [ |
单宁浓度耐受培养基 | 产碱普罗维登斯菌、克斯特菌、普罗威登斯菌和铜绿假单胞菌 | [ |
表1 培养基类型及分离细菌
Table 1 Types of culture medium and isolated bacteria
培养基类型 Types of culture medium | 分离菌株 Isolated strains | 参考文献 References |
---|---|---|
CMC-Na分离培养基 | 芽孢杆菌属、蜡样芽孢杆菌、木聚糖降解球形芽孢杆菌、枯草芽孢杆菌、环状芽孢杆菌、苏云金芽孢杆菌、巨大芽孢杆菌、短小芽孢杆菌、短杆菌、假单胞菌属、铜绿假单胞菌、香茅醇假单胞菌、寡养单胞菌属、纤维单胞菌属、肠杆菌属、棒状杆菌属、微杆菌属、苍白杆菌属、大肠杆菌、鞘氨醇杆菌属、肠球菌属、粪肠球菌、副球菌属、黄色瘤胃球菌、白色瘤胃球菌、链球菌、菠萝泛菌、肺炎克雷伯菌、产酸克雷伯氏菌、溶纤维丁酸弧菌、拜氏羧菌、丁酸梭菌 | [ |
木聚糖唯一碳源培养基 | 溶纤维丁酸弧菌、类芽孢杆菌属 | [ |
酪蛋白主要氮源培养基 | 嗜淀粉拟杆菌、丁酸弧菌属、新月形单胞菌属、毛螺旋菌属、未知种 | [ |
双缩脲、尿素唯一氮源培养基 | 葡萄球菌属、干酪乳杆菌、产气克雷伯氏菌、粪链球菌 | [ |
无氮培养基 | 克雷伯氏菌属、弗兰克氏菌属、芽孢杆菌属 | [ |
LSB培养基 | 痤疮丙酸杆菌、蜡样芽孢杆菌、草乳杆菌、弯曲乳杆菌、吉氏库特菌、粪肠球菌、解淀粉芽孢杆菌、牛链球菌、志贺氏菌、氧化木糖无色杆菌、夏威夷肠球菌、产粘棒状杆菌 | [ |
CDC厌氧血琼脂 | 反刍月形单胞菌、粪肠球菌 | [ |
MRS培养基 | 巴黎链球菌、肠膜明串珠菌、戊糖片球菌、弯曲乳杆菌、耐久肠球菌、海氏肠球菌、鹑鸡肠球菌、屎肠球菌、粪肠球菌 | [ |
SL培养基 | 干酪乳杆菌,黏膜乳杆菌 | [ |
厌氧杆菌培养基 | 产琥珀酸放线杆菌 | [ |
牛肉膏蛋白胨溴甲酚绿培养基 | 放线杆菌属、肠膜明串珠菌 | [ |
单宁浓度耐受培养基 | 产碱普罗维登斯菌、克斯特菌、普罗威登斯菌和铜绿假单胞菌 | [ |
[1] |
Hungate RE. Studies on cellulose fermentation:III. The culture and isolation for cellulose-decomposing bacteria from the rumen of cattle[J]. Journal of Bacteriology, 1947, 53(5):631-645.
doi: 10.1128/jb.53.5.631-645.1947 pmid: 16561317 |
[2] |
Zeineldin M, Barakat R, Elolimy AA, et al. Synergetic action between the rumen microbiota and bovine health[J]. Microbial Pathogenesis, 2018, 124:106-115.
doi: S0882-4010(18)30982-3 pmid: 30138752 |
[3] |
Scharen M, Frahm J, Kersten S, et al. Interrelations between the rumen microbiota and production, behavioral, rumen-fermentation, metabolic, and immunological attributes of dairy cows[J]. Journal of Dairy Science, 2018, 101(5):4615-4637.
doi: 10.3168/jds.2017-13736 URL |
[4] |
DePeters EJ, George LW. Rumen transfaunation[J]. Immunology Letters, 2014, 162(2):69-76.
doi: 10.1016/j.imlet.2014.05.009 URL |
[5] | 王继文, 王立志, 闫天海, 等. 山羊瘤胃与粪便微生物多样性[J]. 动物营养学报, 2015, 27(8):2559-2571. |
Wang JW, Wang LZ, Yan TH, et al. Rumen and fecal microbial diversity of goat[J]. Journal of Animal Nutrition, 2015, 27(8):2559-2571. | |
[6] |
Shen JS, Chai Z, Song LJ, et al. Insertion depth of oral stomach tubes may affect the fermentation parameters of ruminal fluid collected in dairy cows[J]. Journal of Dairy Science, 2012, 95(10):5978-5984.
doi: 10.3168/jds.2012-5499 pmid: 22921624 |
[7] | 李润泫. 牦牛瘤胃产纤维素酶兼性厌氧细菌的筛选、鉴定及发酵研究[D]. 兰州:兰州交通大学, 2015. |
Li RX. Screening, identification and fermentation of cellulase-producing facultative anaerobic bacteria in Rumen of yak[D]. Lanzhou:Lanzhou Jiaotong University, 2015. | |
[8] | 李阳阳. 瘤胃源降解纤维素细菌的分离鉴定及产酶条件优化[D]. 大连:大连工业大学, 2017. |
Li YY. Isolation and identification of rumen cellulose-degrading bacteria and optimization of enzyme production conditions[D]. Dalian:Dalian Polytechnic University, 2017. | |
[9] |
Kim ET, Min K, Kim CH, et al. The effect of plant extracts on in-vitro ruminal fermentation, methanogenesis and methane-related microbes in the rumen[J]. Asian-australasian Journal of Animal Sciences, 2013, 26(4):517-522.
doi: 10.5713/ajas.2012.12480 pmid: 25049817 |
[10] |
Intanoo M, Kongkeitkajorn MB, Pattarajinda V, et al. Isolation and screening of aflatoxin-detoxifying yeast and bacteria from ruminal fluids to reduce aflatoxin B1 contamination in dairy cattle feed[J]. Journal of Applied Microbiology, 2018, 125(6):1603-1613.
doi: 10.1111/jam.2018.125.issue-6 URL |
[11] | Boonsaen P, Poonko S, Kanjanapruetipong J, et al. Isolation and partial characterization of Ruminococcus flavefaciens from the rumen of swamp buffalo[J]. Buffalo Bulletin, 2019, 38(2):311-325. |
[12] | Joshi A, Lanjekar VB, Dhakephalkar PK, et al. Liebetanzomyce-spolymorphus gen. et sp. nov., a new anaerobic fungus(Neocallimastigomycota)isolated from the rumen of a goat[J]. MycoKeys, 2018(40):89. |
[13] |
Abrao FO, Duarte ER, Pessoa MS, et al. Notable fibrolytic enzyme production by Aspergillus spp. isolates from the gastrointestinal tract of beef cattle fed in lignified pastures[J]. PLoS One, 2017, 12(8):e0183628.
doi: 10.1371/journal.pone.0183628 URL |
[14] |
Hanafy RA, Elshahed MS, Youssef NH. Feramyces austinii, gen. nov., sp. nov., an anaerobic gut fungus from rumen and fecal samples of wild Barbary sheep and fallow deer[J]. Mycologia, 2018, 110(3):513-525.
doi: 10.1080/00275514.2018.1466610 pmid: 29969376 |
[15] | 卢玉飞. 瘤胃蛋白质降解相关细菌群落结构的解析[D]. 扬州:扬州大学, 2013. |
Lu YF. Analysis of protein-degrading bacterial community structure in rumen[D]. Yangzhou:Yangzhou University, 2013. | |
[16] | 李君风, 原现军, 董志浩, 等. 西藏地区牦牛瘤胃中兼性厌氧纤维素降解菌的分离鉴定[J]. 草业学报, 2017, 26(6):176-184. |
Li JY, Yuan XJ, Dong ZH. Isolation and identification of facultative anaerobic cellulose-degrading bacteria from rumen of yak in Tibet[J]. Acta Prataculturae Sinica, 2017, 26(6):176-184. | |
[17] |
Zhang YH, Zhang ZY, Dai L, et al. Isolation and characterization of a novel gossypol-degrading bacteria Bacillus subtilis strain RBS[J]. Asian-Australasian Journal of Animal Sciences, 2018, 31(1):63.
doi: 10.5713/ajas.17.0018 URL |
[18] | Habu JB, Ndams IS, Wuyep PA, et al. Isolation and biochemical characterization of anaerobic bacteria strains from discarded ruminal contents of Nigerian breed cattle and preliminary evaluation of its suitability for animal feedstuff[J]. Biotechnology Journal International, 2017:1-13. |
[19] |
Pang J, Liu ZY, Hao M, et al. An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw[J]. Biotechnology for Biofuels, 2017, 10(1):165.
doi: 10.1186/s13068-017-0852-7 URL |
[20] |
Miguel MA, Lee SS, Mamuad LL, et al. Enhancing butyrate production, ruminal fermentation and microbial population through supplementation with Clostridium saccharobutylicum[J]. Journal of Microbiology Biotechnology, 2019, 29(7):1083-1095.
doi: 10.4014/jmb.1905.05016 URL |
[21] |
Yang Y, Zhou MX, Hardwidge PR, et al. Isolation and characterization of N-acyl homoserine lactone-producing bacteria from cattle rumen and swine intestines[J]. Frontiers in Cellular Infection Microbiology, 2018, 8:155.
doi: 10.3389/fcimb.2018.00155 URL |
[22] | 胡盼, 夏成, 吴凌, 等. 一株瘤胃厌氧菌Actinomyces ruminicola的分离鉴定[J]. 中国微生态学杂志, 2009, 21(9):809-811. |
Hu P, Xia C, Wu L, et al. Isolation and characterization of one strain of rumen anaerobic bacteria Actinomyces ruminicola[J]. Chinease Journal of Microbiology, 2009, 21(9):809-811. | |
[23] | 武心镇, 高玉霞, 欧海龙, 等. 奶牛瘤胃中反刍兽月形单胞菌的分离鉴定[J]. 动物医学进展, 2008(10):25-28. |
Wu XZ, Gao YX, Ou HL, et al. Isolation and characterization of Selenomonas ruminantium fron the rumen of Dairy Cows[J]. Progress in Veterinary Medicine, 2008(10):25-28. | |
[24] | 赵明娟. 12株瘤胃细菌的分离及痤疮丙酸杆菌对瘤胃微生物发酵的影响[D]. 大庆:黑龙江八一农垦大学, 2008. |
Zhao MJ. Isolation of 12 strains of rumen bacteria and effects of Propionibacterium acnes on rumen microbial fermentation[D]. Daqing:Heilongjiang Bayi Agricultural University, 2008. | |
[25] | 林聪宏. 贵州黑山羊瘤胃溶纤维丁酸弧菌分离鉴定[D]. 贵阳:贵州大学, 2016. |
Lin CH. Isolation and identification of rumen Butyrivibrio fibrisolvens from Guizhou Black Goat[D]. Guiyang:Guizhou University, 2016. | |
[26] |
Palakawong NAS, Pristas P, Hrehova L, et al. Actinomyces succiniciruminis sp. nov. and Actinomyces glycerinitolerans sp. nov., two novel organic acid-producing bacteria isolated from rumen[J]. Systematic Applied Microbiology, 2016, 39(7):445-452.
doi: 10.1016/j.syapm.2016.08.001 URL |
[27] | 刘玉承. 32株瘤胃纤维降解细菌的分离鉴定与保藏研究[D]. 呼和浩特:内蒙古农业大学, 2007. |
Liu YC. Isolation, identification and preservation of 32 fiber-degrading strains in rumen[D]. Hohhot:Inner Mongolia Agriculture University, 2007. | |
[28] |
Zhang L, Chung J, Jiang QQ, et al. Characteristics of rumen microorganisms involved in anaerobic degradation of cellulose at various pH values[J]. RSC Advances, 2017, 7(64):40303-40310.
doi: 10.1039/C7RA06588D URL |
[29] | 韦海婷. 梅花鹿瘤胃纤维素降解菌的分离鉴定及降解特性研究[D]. 北京:中国农业科学院, 2018. |
Wei HT. Isolation, identification and degradation characteristics of rumen cellulose-degrading bacteria from sika deer[D]. Beijing:Chinese Academy of Agricultural Sciences, 2018. | |
[30] | 杨龙龙. 饲用乳酸菌的分离筛选及其活菌制剂在围产期奶牛和新生犊牛保健中的初步应用[D]. 石河子:石河子大学, 2014. |
Yang LL. Isolation and screening of feed lactic acid bacteria and preliminary application of live bacterial preparations in perinatal dairy cows and newborn calves[D]. Shihezi:Shihezi University, 2014. | |
[31] |
Latham EA, Pinchak WE, Trachsel J, et al. Isolation, characterization and strain selection of a Paenibacillus species for use as a probiotic to aid in ruminal methane mitigation, nitrate/nitrite detoxification and food safety[J]. Bioresource Technology, 2018, 263:358-364.
doi: 10.1016/j.biortech.2018.04.116 URL |
[32] |
Kim MK, Kim ET, Kim SB, et al. Kurthia ruminicola sp. nov., isolated from the rumen contents of a Holstein cow[J]. Journal of Microbiology, 2018, 56(1):36-41.
doi: 10.1007/s12275-018-7285-2 URL |
[33] | Rawway M, Ali SG, Badawy AS. Isolation and identification of cellulose degrading bacteria from different sources at Assiut Governorate(Upper Egypt)[J]. Journal of Ecology of Health & Environment, 2018, 6:15. |
[34] | 苏少锋, 呼和, 王超, 等. 瘤胃枯草芽胞杆菌的分离鉴定及其木聚糖酶基因的克隆和在乳酸球菌中表达的研究[J]. 畜牧与饲料科学, 2013, 34(9):5-9, 13. |
Su SF, Hu H, Wang C. Isolation and identification of Bacillus subtilis and cloning of xylanase gene and its expression in Lactococcus[J]. Animal Husbandry and Feed Science, 2013, 34(9):5-9, 13. | |
[35] |
Lee GH, Rhee MS, Chang DH, et al. Oscillibacterruminantium sp. nov., isolated from the rumen of Korean native cattle[J]. International Journal of Systematic Evolutionary Microbiology, 2013, 63(6):1942-1946.
doi: 10.1099/ijs.0.041749-0 URL |
[36] | Pimentel MFA, Paula DAJD, Rietcorrea F, et al. Detection and characterization of bovine rumen microorganisms resistant to sodium fluoroacetate[J]. Acta Scientiae Veterinariae, 2019, 47:1627. |
[37] |
Sousa A, Ramiro RS, Barroso-Batista J, et al. Recurrent reverse evolution maintains polymorphism after strong bottlenecks in commensal gut bacteria[J]. Molecular Biology and Evolution, 2017, 34(11):2879-2892.
doi: 10.1093/molbev/msx221 pmid: 28961745 |
[38] |
Oskouei DD, Bekmen N, Ellidokuz H, et al. Evaluation of different cryoprotective agents in maintenance of viability of Helicobacter pylori in stock culture media[J]. Brazilian Journal of Microbiology, 2010, 41(4):1038-1046.
doi: 10.1590/S1517-83822010000400023 URL |
[39] | 郭玲玲. 微生物菌种保藏方法及关键技术[J]. 微生物学杂志, 2019, 39(3):105-108. |
Guo LL. Preservation methods and preservation key technologu of microbial strain seed[J]. Journal of Microbiology, 2019, 39(3):105-108. | |
[40] | Rojas-Tapias D, Ortiz-Vera M, Rivera D, et al. Evaluation of three methods for preservation of Azotobacter chroococcum and Azotobacter vinelandii[J]. Universitas Scientiarum, 2013, 18(2):129-139. |
[41] |
Morgan CA, Herman N, White PA, et al. Preservation of micro-organisms by drying;a review[J]. Journal of Microbiological Methods, 2006, 66(2):183-93.
pmid: 16632005 |
[42] |
Miyamoto-Shinohara Y, Imaizumi T, Sukenobe J, et al. Survival rate of microbes after freeze-drying and long-term storage[J]. Cryobiology, 2000, 41(3):251-255.
pmid: 11161557 |
[43] | Caldwell DR, Bryant MP. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria[J]. Applied and Environmental Microbiology, 1966, 14(5):794-801. |
[44] | 李昊. 固氮菌和纤维素分解菌的分离及混合添加对瘤胃发酵的影响[D]. 哈尔滨:东北农业大学, 2013. |
Li H. Isolation of nitrogen-fixing bacteria and cellulolytic bacteria and the effect of its mixed addition on rumen fermentation[D]. Harbin:Northeast Agriculture Univesity, 2013. | |
[45] |
Sari WN, Safika, Darmawi, et al. Isolation and identification of a cellulolytic Enterobacter from rumen of Aceh cattle[J]. Veterinary World, 2017, 10(12):1515-1520.
doi: 10.14202/vetworld. URL |
[46] | 朱继红, 孙满吉, 张永根. 瘤胃纤维降解菌株的筛选[J]. 饲料博览, 2012(1):5-8. |
Zhu JH, Sun MJ, Zhang YG. Screening of the ruminal rice straw cellulolytic bacteria[J]. Feed Review, 2012(1):5-8. | |
[47] | 朱继红. 瘤胃兼性厌氧CDB的分离鉴定及其对奶牛生产性能的研究[D]. 哈尔滨:东北农业大学, 2012. |
Zhu JH. Study on isolation, characterization and production performance of ruminal facultatively anaerobic cellulose-decomposing bacteria in Holstein Cows[D]. Harbin:Northeast Agricultural University, 2012. | |
[48] | 买尔哈巴·艾合买提. 瘤胃中纤维素分解菌的分离、鉴定及其在青贮饲料中的应用研究[D]. 乌鲁木齐:新疆大学, 2013. |
Maierhaba A. Isolation and identification of cellulose decomposing bacteria in rumen and its application in silage[D]. Urumqi:Xinjiang University, 2013. | |
[49] | 蒋培培, 刘福音, 邹智坤, 等. 牦牛瘤胃中产纤维素酶芽孢杆菌的分离鉴定[J]. 中国畜牧兽医, 2016, 43(2):527-534. |
Jiang PP, Liu JC, Zou ZK, et al. Isolation and identification of Cellulase Producing Bacillus from rumen of yak[J]. Chinese Animal Husbandry and Veterinary, 2016, 43(2):527-534. | |
[50] | 侯进慧, 刘彤, 李同祥. 产纤维素酶菌株Pantoea ananatis P5的筛选鉴定和活性研究[J]. 徐州工程学院学报:自然科学版, 2013, 28(4):80-84. |
Hou JH, Liu T, Li TX. Screening, identification and activity of Cellulase Producing Strain Pantoea ananatis P5[J]. Journal of Xuzhou Institute of Technology:Natural Science Edition, 2013, 28(4):80-84. | |
[51] | 王炳晓. 奶牛瘤胃兼性厌氧纤维素分解细菌的分离鉴定及其产酶研究[D]. 泰安:山东农业大学, 2008. |
Wang BX. Isolation, identification and enzyme production of facultative anaerobic cellulolytic bacteria from rumen of dairy cows[D]. Tai’an:Shandong Agricultural University, 2008. | |
[52] | 王炳晓, 柴同杰, 苏鹏程, 等. 奶牛瘤胃兼性厌氧纤维素分解菌的分离鉴定[J]. 西北农林科技大学学报:自然科学版, 2009, 37(3):35-42. |
Wang BX, Chai TJ, Su PC, et al. Isolation and identification of facultative anaerobic cellulolytic bacteria in rumen of dairy cows[J]. Journal of Northwest A & F University:Natural Science Edition, 2009, 37(3):35-42. | |
[53] | 彭忠利, 邹智坤, 曾泽, 等. 藏绵羊瘤胃中产纤维素酶芽胞杆菌的分离鉴定[J]. 动物医学进展, 2016, 37(4):70-73. |
Peng ZL, Zou ZK, Zeng Z, et al. Isolation and identification of Cellulase Producing Bacillus from rumen of Tibetan sheep[J]. Progress in Animal Medicine, 2016, 37(4):70-73. | |
[54] | 崔小蕾. 功能性微生物的筛选[D]. 大连:大连工业大学, 2017. |
Cui XL. Screening of functional microorganisms[D]. Dalian:Dalian University of Technology, 2017. | |
[55] | 周非帆. 瘤胃纤维降解细菌的分离鉴定及纤维降解特性研究[D]. 呼和浩特:内蒙古农业大学, 2014. |
Zhou FF. Isolation and identification of rumen fiber degrading bacteria and study on fiber degradation characteristics[D]. Hohhot:Inner Mongolia Agricultural University, 2014. | |
[56] |
Sewell GW, Aldrich HC, Williams D, et al. Isolation and characterization of xylan-degrading strains of butyrivibrio fibrisolvens from a napier grass-fed anaerobic digester[J]. Applied Environmental Microbiology, 1988, 54(5):1085-1090.
doi: 10.1128/aem.54.5.1085-1090.1988 URL |
[57] | 张贝贝. 牛瘤胃细菌产木聚糖酶菌株的筛选及其酶学性质的研究[D]. 郑州:河南农业大学, 2013. |
Zhang BB. Screening of xylanase-producing strain from bovine rumen and its enzymatic properties[D]. Zhengzhou:Henan Agricultural University, 2013. | |
[58] | Blackburn TH, Hobson PN. Further studies on the isolation of proteolytic bacteria from the sheep rumen[J]. Microbiology, 1962, 29(1):69-81. |
[59] | Cook AR. Urease activity in the rumen of sheep and the isolation of ureolytic bacteria[J]. Microbiology, 1976, 92(1):32-48. |
[60] | Bellingham F, Bernstein M. The isolation of a biuret degrading bacterium from the rumen of a sheep[J]. Journal of Applied Microbiology, 1973, 36(1):183-186. |
[61] | 吴凌, 赵明娟, 夏成, 等. 痤疮丙酸杆菌的分离鉴定及其对瘤胃微生物发酵的影响[J]. 微生物学报, 2009, 49(2):168-173. |
Wu L, Zhao MJ, Xia C, et al. Isolation and identification of Propionibacterium acnes and its effect on rumen microbial fermentation[J]. Acta microbiologica Sinica, 2009, 49(2):168-173. | |
[62] | 赵明娟, 吴凌, 夏成, 等. 奶牛瘤胃中粪肠球菌的分离鉴定[J]. 黑龙江畜牧兽医, 2008(9):65-66. |
Zhao MJ, Wu L, Xia C, et al. Isolation and identification of Enterococcus faecalis from rumen of dairy cows[J]. Heilongjiang Animal Husbandry and Veterinary, 2008(9):65-66. | |
[63] | 于鸿玲. 反刍月形单胞菌及其转座工程菌对羊瘤胃发酵的影响[D]. 哈尔滨:东北农业大学, 2006. |
Yu HL. Effects of lunamonas ruminant and its transposable engineering bacteria on rumen fermentation of sheep[D]. Harbin:Northeast Agricultural University, 2006. | |
[64] | 王德光. 饲用菌种(株)的分离鉴定及复合菌剂降解玉米皮—统糠粗纤维的效果研究[D]. 杨凌:西北农林科技大学, 2017. |
Wang DG. Isolation and identification of feeding species(strains)and effect of compound bacteria on degradation of Corn Meal-rice bran[D]. Yangling:Northwest A&F University, 2017. | |
[65] | 徐凤, 张焕容, 张兴民, 等. 不同年龄山羊胃肠道乳酸菌的分离鉴定[J]. 黑龙江畜牧兽医, 2017(5):108-110. |
Xu F, Zhang HR, Zhang XM. Isolation and identification of lactic acid bacteria from gastrointestinal tract of goats of different ages[J]. Heilongjiang Animal Science and Veterinary Medicine, 2017(5):108-110. | |
[66] | 龙淼, 逄晓阳, 朱连勤, 等. 牛瘤胃耐酸乳酸杆菌的分离与鉴定[J]. 中国兽医学报, 2009, 29(3):288-291. |
Long M, Fu XY, Zhu LQ, et al. Isolation and identification of acid fast Lactobacillus from rumen of cattle[J]. Chinese Journal of Veterinary Medicine, 2009, 29(3):288-291. | |
[67] | 欧海龙. 产琥珀酸放线杆菌的分离鉴定及发酵特性[D]. 大庆:黑龙江八一农垦大学, 2009. |
Ou HL. Isolation, identification and fermentation characteristics of Actinobacillus succinogenes[D]. Daqing:Heilongjiang Bayi Agricultural University, 2009. | |
[68] | 吴凌, 欧海龙, 夏成, 等. 产琥珀酸放线杆菌的分离鉴定及其对瘤胃微生物发酵的影响[J]. 中国微生态学杂志, 2009, 21(8):737-740, 742. |
Wu L, Ou HL, Xia C, et al. Isolation and identification of Actinobacillus succinogenes and its effect on rumen microbial fermentation[J]. Chinese Journal of Microbiology, 2009, 21(8):737-740, 742. | |
[69] | 李兴江, 潘丽军, 姜绍通, 等. 牛瘤胃中琥珀酸产生菌的分离鉴定研究[J]. 合肥工业大学学报:自然科学版, 2007, 30(10):1327-1330. |
Li XJ, Pan LJ, Jiang ST. Isolation and identification of succinic acid producing bacteria from rumen of bovine[J]. Journal of Hefei University of Technology:Natural Science, 2007, 30(10):1327-1330. | |
[70] | 陈晓晖. 琥珀酸产生菌的分离鉴定、诱变选育及发酵条件优化[D]. 合肥:合肥工业大学, 2007. |
Chen XH. Isolation, identification, mutation breeding and optimal fermentation conditions of succinic acid producing bacteria[D]. Hefei:Hefei University of Technology, 2007. | |
[71] | 李兴芳, 徐雯, 张胜男, 等. 山羊瘤胃中单宁降解菌的分离、筛选和鉴定[J]. 动物营养学报, 2015, 27(6):1921-1927. |
Li XF, Xu W, Zhang SN. Isolation, screening and identification of tannin degrading bacteria in rumen of goats[J]. Chinese Journal of Animal Nutrition, 2015, 27(6):1921-1927. | |
[72] |
Macpheeson MJ. Isolation and identification of amylolytic Streptococci from the rumen of the sheep[J]. Journal of Pathology Bacteriology, 1953, 66:95-102.
doi: 10.1002/(ISSN)1555-2039 URL |
[73] |
Jung D, Seo D, Kim G, et al. The effect of resistant starch(RS)on the bovine rumen microflora and isolation of RS-degrading bacteria[J]. Applied Microbiology Biotechnology, 2018, 102(11):4927-4936.
doi: 10.1007/s00253-018-8971-z URL |
[74] | Hobson PN, Mann SOJ. The isolation of glycerol-fermenting and lipolytic bacteria from the rumen of the sheep[J]. Microbiology, 1961, 25(2):227-240. |
[75] | Sanusi OA. Identification and enzyme production of a cellulolytic Bacillus-strain isolated from moose(Alces alces)rumen[D]. Elverlum: Hedmark University College, 2013. |
[76] |
Hungate RE. The anaerobic mesophilic cellulolytic bacteria[J]. Bacteriol Rev, 1950, 14(1):1-49.
doi: 10.1128/br.14.1.1-49.1950 URL |
[77] |
Kolinko S, Wu YW, Tachea F, et al. A bacterial pioneer produces cellulase complexes that persist through community succession[J]. Nature Microbiology, 2018, 3(1):99-107.
doi: 10.1038/s41564-017-0052-z URL |
[78] |
Reichardt W. Impact of the Antarctic benthic fauna on the enrichment of biopolymer degrading psychrophilic bacteria[J]. Microbial Ecology, 1988, 15(3):311-321.
doi: 10.1007/BF02012644 pmid: 24201408 |
[79] |
Jung HG, Varel VH. Influence of forage type on ruminal bacterial populations and subsequent in vitro fiber digestion[J]. Journal of Dairy Science, 1988, 71(6):1526-1535.
pmid: 2841364 |
[80] |
McDonald JE, Rooks DJ, McCarthy AJ, Methods for the isolation of cellulose-degrading microorganisms[J]. Methods in Enzymology, 2012, 510:349-374.
doi: 10.1016/B978-0-12-415931-0.00019-7 pmid: 22608736 |
[81] |
Butterworth JP, Bell SE, Garvock MG. Isolation and properties of the xylan-fermenting bacterium 11[J]. Biochemical Journal, 1960, 74(1):180-182.
doi: 10.1042/bj0740180 URL |
[82] |
Nyonyo T, Shinkai T, Mitsumori M. Improved culturability of cellulolytic rumen bacteria and phylogenetic diversity of culturable cellulolytic and xylanolytic bacteria newly isolated from the bovine rumen[J]. FEMS Microbiology Ecology, 2014, 88(3):528-537.
doi: 10.1111/1574-6941.12318 pmid: 24612331 |
[83] |
Simunek J, Killer J, Sechovcova H, et al. Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer(Cervus elaphus)closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola[J]. Folia Microbiologica, 2018, 63(3):391-399.
doi: 10.1007/s12223-017-0577-9 URL |
[84] |
Ghali MB, Scott PT, Jassim RAM. Characterization of Streptococcus bovis from the rumen of the dromedary camel and Rusa deer[J]. Letters in Applied Microbiology, 2004, 39(4):341-346.
pmid: 15355536 |
[85] |
Jensen RG, Smith KL, Edmondson JE, et al. The characteristics of some rumen Lactobacilli[J]. Journal of Bacteriology, 1956, 72(2):253-258.
doi: 10.1128/jb.72.2.253-258.1956 URL |
[86] |
Mcsweeney CS, Palmer B, Bunch RJ, et al. Isolation and characterization of proteolytic ruminal bacteria from sheep and goats fed the tannin-containing shrub legume Calliandra calothyrsus[J]. Applied Environmental Microbiology, 1999, 65(7):3075-3083.
doi: 10.1128/AEM.65.7.3075-3083.1999 URL |
[87] |
John A, Isaacson HR, Bryant MP. Isolation and characteristics of a ureolytic strain of Selenomonas ruminantium[J]. Journal of Dairy Science, 1974, 57(9):1003-1014.
pmid: 4213200 |
[88] | 刘欢. 甲烷氧化菌的分离与鉴定及其活菌制剂对奶牛瘤胃内甲烷排放量的影响[D]. 长春:吉林大学, 2016. |
Liu H. Isolation and identification of methanotrophic bacteria and effect of its active agents on methane emission of dairy cows[D]. Changchun:Jilin University, 2016. | |
[89] |
Sharma D, Mal G, Kannan A, et al. Degradation of euptox A by tannase-producing rumen bacteria from migratory goats[J]. Journal of Applied Microbiology, 2017, 123(5):1194-1202.
doi: 10.1111/jam.13563 pmid: 28801990 |
[90] | Camboim EK, Almeida AP, Tadra-Sfeir MZ, et al. Isolation and identification of sodium fluoroacetate degrading bacteria from caprine rumen in Brazil[J]. The Scientific World Journal, 2012(1):178254. |
[91] |
Faruque AJMO, Jarvis BDW, Hawke JC. Studies on rumen metabolism. VIII. Characteristics of lipases in rumen contents and in rumen bacteria[J]. Journal of the Science of Food Agriculture, 1974, 25(4):439-449.
doi: 10.1002/(ISSN)1097-0010 URL |
[92] | Henderson C. The isolation and characterization of strains of lipolytic bacteria from the ovine rumen[J]. Journal of Applied Microbiology, 1975, 39(2):101-109. |
[93] | 韩生义, 刘晓丽, 张国权, 等. 牦牛瘤胃中产脂肪酶微生物的分离与鉴定[J]. 微生物学通报, 2019, 46(9):2292-2301. |
Han SY, Liu XL, Zhang GQ, et al. Isolation and identification of lipase-producing bacteria from rumen of yak[J]. Microbiology China, 2019, 46(9):2292-2301. | |
[94] | Priji P, Unni KN, Sajith S, et al. Production, optimization, and partial purification of lipase from Pseudomonas sp. strain BUP6, a novel rumen bacterium characterized from Malabari goat[J]. Biotechnology Applied Biochemistry, 2015, 62(1):71-78. |
[95] |
Priji P, Sajith S, Unni KN, et al. Pseudomonas sp. BUP6, a novel isolate from Malabari goat produces an efficient rhamnolipid type biosurfactant[J]. Journal of Basic Microbiology, 2017, 57(1):21-33.
doi: 10.1002/jobm.v57.1 URL |
[96] | 王铁良, 于颖, 刘秀萍. 牛源性蛋氨酸生产菌的分离筛选及发酵条件优化[J]. 畜牧与兽医, 2017, 49(8):57-61. |
Wang TL, Yu Y, Liu XP. Isolation, screening and optimal fermentation conditions of methionine producing bacteria from bovine[J]. Animal Husbandry and Veterinary Medicine, 2017, 49(8):57-61. | |
[97] | Maluszynska GM, Janotabassalik L. A cellulolytic rumen bacterium, Micromonospora ruminantium sp. nov[J]. Microbiology, 1974, 82(1):57-65. |
[98] |
Chun BH, Lee HJ, Jeong SE, et al. Leucobacter ruminantium sp. nov., isolated from the bovine rumen[J]. International Journal of Systematic Evolutionary Microbiology, 2017, 67(8):2634-2639.
doi: 10.1099/ijsem.0.002003 URL |
[99] |
Jeon BS, Kim S, Sang B. Megasphaera hexanoica sp. nov., a medium-chain carboxylic acid-producing bacterium isolated from a cow rumen[J]. International Journal of Systematic Evolutionary Microbiology, 2017, 67(7):2114-2120.
doi: 10.1099/ijsem.0.001888 URL |
[100] | Gylswyk NOV, Hoffman JPL. Characteristics of cellulolytic cillobacteria from the rumens of sheep fed teff(Eragrostis tef)hay diets[J]. Microbiology, 1970, 60(3):381-386. |
[101] | Gylswyk NOV, Roche C. Characteristics of Ruminococcus and cellulolytic Butyrivibrio species from the rumens of sheep fed differently supplemented teff(Eragrostis tef)hay diets[J]. Microbiology, 1970, 64(1):11-17. |
[102] | Jarvis BDW, Annison EF. Isolation, classification and nutritional requirements of cellulolytic cocci in the sheep rumen[J]. Microbiology, 1967, 47(2):295-307. |
[103] |
Luna L, Hernández D, Silva HV, et al. Isolation, biochemical characterization, and phylogeny of a cellulose-degrading ruminal bacterium[J]. Revista Colombiana de Ciencias Pecuarias, 2019, 32(2):117-125.
doi: 10.17533/udea.rccp.v32n2a05 URL |
[104] | Kameshwar AKS, Qin WS. Isolation and screening of cellulose-degrading microorganisms from different ecological niches, In: Methods in Molecular Biology[M]. New York: Humana Press, 2018:47-56. |
[105] |
King KW, Smith PH. Comparisons of two media proposed for the isolation of bacteria from the rumen[J]. Journal of Bacteriology, 1955, 70(6):726.
doi: 10.1128/jb.70.6.726-729.1955 URL |
[106] | Gylswyk NOV. A comparison of two techniques for counting cellulolytic rumen bacteria[J]. Microbiology, 1970, 60(2):191-197. |
[107] |
Battumur U, Lee M, Bae GS, et al. Isolation and characterization of a new Methanoculleus bourgensis strain KOR-2 from the rumen of Holstein steers[J]. Asian-Australasian Journal of Animal Sciences, 2019, 32(2):241.
doi: 10.5713/ajas.18.0409 URL |
[1] | 朱建峰, 杨秀艳, 武海雯, 张华新. 植物种子萌发期耐盐碱性提高技术研究进展[J]. 生物技术通报, 2020, 36(2): 158-168. |
[2] | 刘雪婷, 元虹懿, 张明海, 关伟军. 鸭脂肪间充质干细胞分离培养与生物学特性[J]. 生物技术通报, 2016, 32(8): 122-128. |
[3] | 虞飞, 李斌, 张晶晶, 丁海麦, 张学明. 重组人gdnf乳腺特异表达载体转染牛胎儿成纤维细胞的研究[J]. 生物技术通报, 2014, 0(8): 102-107. |
[4] | 吕春荣, 熊慧, 宫雪莲, 关伟军, 马月辉. 北京鸭表皮干细胞的分离培养[J]. 生物技术通报, 2013, 0(11): 93-97. |
[5] | 褚姣娇;徐正会;. 蚂蚁分子系统学研究进展[J]. , 2009, 0(07): 42-47. |
[6] | 张艳艳;王颖;陈莉娜;李林凤;靳亚平;关伟军;马月辉;. 禽类胚胎生殖细胞的研究[J]. , 2008, 0(06): 40-43. |
[7] | 杨恕玲;董雅娟;柏学进;程明;李建栋;王雪红;董方圆;. 昆明小鼠胚胎干细胞的体外分离培养[J]. , 2006, 0(S1): 422-426. |
[8] | . 《生物技术通报》稿约[J]. , 2002, 0(01): 57-57. |
[9] | 李思经. 食品生产的未来[J]. , 1995, 0(05): 30-30. |
[10] | 李思经;. 重组瘤胃细菌有利于饲料消化[J]. , 1992, 0(09): 5-6. |
[11] | 李思经. 联合研究新型煤产物[J]. , 1991, 0(06): 24-25. |
[12] | 朱遐;. 莫斯科新建生物技术研究所[J]. , 1991, 0(02): 29-30. |
[13] | 王璋瑜;. 人蛋白抑制小鼠中人肿瘤的生长[J]. , 1990, 0(12): 17-17. |
[14] | 王璋瑜;. 以生物学方法生产丁醇[J]. , 1990, 0(06): 9-10. |
[15] | 李思经;. 除草剂抗性作物的大田试验[J]. , 1990, 0(05): 16-17. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||