鸭脂肪间充质干细胞分离培养与生物学特性

doi:10.13560/j.cnki.biotech.bull.1985.2016.08.019

生物技术通报 ›› 2016, Vol. 32 ›› Issue (8): 122-128.doi: 10.13560/j.cnki.biotech.bull.1985.2016.08.019

鸭脂肪间充质干细胞分离培养与生物学特性

刘雪婷^{1, 2}, 元虹懿^{1, 2}, 张明海¹, 关伟军²

1. 东北林业大学,哈尔滨 150040;
2. 中国农业科学院北京畜牧兽医研究所,北京 100193

修回日期:2015-11-02 出版日期:2016-08-25 发布日期:2016-08-25
作者简介:刘雪婷,女,硕士研究生,研究方向：野生动物生态学、保护与管理学;E-mail：esselxt@126.com
基金资助:
国家自然科学基金项目（31472099）,中国农业科学院科技创新工程项目（cxgc-ias-01）

Isolation and Biological Characterization of Duck Adipose-derived Mesenchymal Stem Cells

LIU Xue-ting^{1, 2}, YUAN Hong-yi^{1, 2}, ZHANG Ming-hai¹, GUAN Wei-jun²

1. Northeast Forestry University,Harbin 150040;
2. Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193

Revised:2015-11-02 Published:2016-08-25 Online:2016-08-25

摘要/Abstract

摘要： 建立禽类脂肪来源的间充质干细胞（adipose-derived stem cells,ADSCs）体外培养体系,探索其生物学特性和多分化潜能。通过酶消化法分离18日龄鸭胚ADSCs,绘制生长曲线,通过RT-PCR、免疫荧光和流式细胞术阳性率检测对ADSCs进行鉴定,诱导ADSCs向成骨细胞和脂肪细胞分化。结果表明,鸭胚胎ADSCs体外增殖能力良好;阳性表达CD29,CD44,CD105,表达率分别为95.39%、94.53%和98.19%;经体外诱导后可分化为成骨细胞和脂肪细胞。研究认为在适宜的培养条件下,体外培养的ADSCs能够稳定增殖并具有多分化潜能,可作为组织工程的种子细胞进行保存。

关键词: 脂肪间充质干细胞, 分离培养, 体外诱导分化, 多分化潜能, 生物学特性

Abstract: This study aims to establish in vitro culture system for the poultry adipose-derived stem cells（ADSCs）,to study the biological characteristics and the multi-differentiation potential. Enzyme digestion method was applied to separate Beijing duck 18-day-old embryos ADSCs and to draw the growth curve. ADSCs were identified by RT-PCR,immunofluorescence and flow cytometric analysis. Our results suggested that the ADSCs had solid proliferative activity and were positive for CD29,CD44 and CD105,the rate of CD29+,CD44+,and CD105+ ADSCs was 95.39%,94.53%,98.19%,respectively. ADSCs differentiated into adipocytes and osteoblasts by induced differentiation in vitro. In summary,under appropriate experimental circumstances,Beijing duck embryo ADSCs have a strong self-renewing ability and multi-differentiation potential in vitro,and it can be an ideal type of seed cell for cellular transplantation therapy.

Key words: adipose-derived stem cells, isolation, in vitro directional differentiation, multi-differentiation potential, the biological characteristics

刘雪婷, 元虹懿, 张明海, 关伟军. 鸭脂肪间充质干细胞分离培养与生物学特性[J]. 生物技术通报, 2016, 32(8): 122-128.

LIU Xue-ting, YUAN Hong-yi, ZHANG Ming-hai, GUAN Wei-jun. Isolation and Biological Characterization of Duck Adipose-derived Mesenchymal Stem Cells[J]. Biotechnology Bulletin, 2016, 32(8): 122-128.

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鸭脂肪间充质干细胞分离培养与生物学特性

Isolation and Biological Characterization of Duck Adipose-derived Mesenchymal Stem Cells

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