广藿香百秋李醇分子调控及合成生物学研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2021-0126

摘要/Abstract

摘要：

百秋李醇是广藿香主要活性成分之一,也是《中国药典》中评价广藿香药材的重要指标,可通过天然提取或合成生物学工程两种方式获得。广藿香中百秋李醇含量低,是其规模化应用的瓶颈。本文从百秋李醇的合成途径、分子调控机制和合成生物学工程三方面综述近年来相关研究进展,为百秋李醇的开发与应用提供参考。

关键词: 广藿香, 百秋李醇, 分子调控机制, 合成生物工程

Abstract:

Patchoulol is one of the main active components in Pogostemon cablin,and it is an important index for evaluating P. cablin in Chinese Pharmacopoeia. It can be obtained by natural extraction or synthetic biological engineering. The little amount of patchoulol in P. cablin is the bottleneck of its large-scale application. This paper reviews the research progress on patchoulol synthesis pathway,molecular regulation mechanism and synthetic biology engineering,providing reference for patchoulol development and application.

Key words: Pogostemon cablin, patchoulol, mechanism of molecular regulation, synthetic biology engineering

张婵, 姚广龙, 张军锋, 于靖, 杨东梅, 陈萍, 吴友根. 广藿香百秋李醇分子调控及合成生物学研究进展[J]. 生物技术通报, 2021, 37(8): 55-64.

ZHANG Chan, YAO Guang-long, ZHANG Jun-feng, YU Jing, YANG Dong-mei, CHEN Ping, WU You-gen. Research Progress on Patchoulol Molecular Regulation and Synthetic Biology in Pogostemon cablin[J]. Biotechnology Bulletin, 2021, 37(8): 55-64.

图/表 3

参考文献 52

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基因 Gene	全长 Full length/bp	预测蛋白特征 Predicted protein characteristics	GenBank登录号 GeneBank accession No.	文献 Reference
PatHMGR	2209	425 aa,疏水性蛋白,两个跨膜区且不含信号肽	MK591025	[20]
PatIPI	1315	292 aa,亲水性蛋白,无跨膜区具信号肽	MW238535	[19]
PatFPS	1177	349 aa,亲水性蛋白,无跨膜区,不含信号肽,含DDXXD保守结构域	-	[21]
	1050		-	[17]
	1050		-	[18]
	1659	552-570 aa,亲水性蛋白,无跨膜区,无信号肽,含DDXXD保守序列,2 个倍半萜合酶结构域	AY508730.1	[22]
	1659		KF983531	[23]
PatPTS	1659		KP694233.1	[24]
	1713		-	[25]
	1659		-	[26]

基因 Gene	全长 Full length/bp	预测蛋白特征 Predicted protein characteristics	GenBank登录号 GeneBank accession No.	文献 Reference
PatHMGR	2209	425 aa,疏水性蛋白,两个跨膜区且不含信号肽	MK591025	[20]
PatIPI	1315	292 aa,亲水性蛋白,无跨膜区具信号肽	MW238535	[19]
PatFPS	1177	349 aa,亲水性蛋白,无跨膜区,不含信号肽,含DDXXD保守结构域	-	[21]
	1050		-	[17]
	1050		-	[18]
	1659	552-570 aa,亲水性蛋白,无跨膜区,无信号肽,含DDXXD保守序列,2 个倍半萜合酶结构域	AY508730.1	[22]
	1659		KF983531	[23]
PatPTS	1659		KP694233.1	[24]
	1713		-	[25]
	1659		-	[26]

年份 Year	生物 Organisms	特点 Characteristics	百秋李醇产量 Productivities of patchoulol	文献 Reference
2020	酿酒酵母 Saccharomyces cerevisiae	真菌,高生长速率, 糖代谢速率快,耐受性高。	42.1 mg·L^-1	[41]
2019			466.8 mg·L^-1	[9]
2015			41.6 mg·L^-1	[42]
2011			40.9 mg·L^-1	[43]
2008			11.5 mg·L^-1	[44]
2018	谷氨酸棒杆菌 Corynebacterium glutamicum	革兰氏阳性细菌,细胞密度高和容积生产率高。	60 mg·L^-1	[2]
2019	荚膜红细菌 Rhodobacter capsulatus	革兰氏阴性细菌,营光合自养,	24 mg·L^-1	[45]
2006	烟草 Nicotiana tobacum	高等植物,多年生草本常用萜类生产底盘。	30 µg·g^-1FW	[46]
2016	莱茵衣藻 Chlamydomonas reinhardtii	低等植物,单细胞真核藻类,细胞结构简单。	922 µg·g^-1 CDW	[47]
2020	小立碗藓 Physcomitrella patens	低等植物,萜类背景简单,极高同源重组率。	32.9 µg·g^-1 CDW	[48]
2014	小立碗藓 Physcomitrella patens	低等植物,萜类背景简单,极高同源重组率。	59 µg·g^-1 CDW	[49]
2018	地钱 Marchantia polymorpha	低等植物,基因组小、冗余基因少、易繁殖培养。	1 000.36 μg·g^-1CDW	[50]

年份 Year	生物 Organisms	特点 Characteristics	百秋李醇产量 Productivities of patchoulol	文献 Reference
2020	酿酒酵母 Saccharomyces cerevisiae	真菌,高生长速率, 糖代谢速率快,耐受性高。	42.1 mg·L^-1	[41]
2019			466.8 mg·L^-1	[9]
2015			41.6 mg·L^-1	[42]
2011			40.9 mg·L^-1	[43]
2008			11.5 mg·L^-1	[44]
2018	谷氨酸棒杆菌 Corynebacterium glutamicum	革兰氏阳性细菌,细胞密度高和容积生产率高。	60 mg·L^-1	[2]
2019	荚膜红细菌 Rhodobacter capsulatus	革兰氏阴性细菌,营光合自养,	24 mg·L^-1	[45]
2006	烟草 Nicotiana tobacum	高等植物,多年生草本常用萜类生产底盘。	30 µg·g^-1FW	[46]
2016	莱茵衣藻 Chlamydomonas reinhardtii	低等植物,单细胞真核藻类,细胞结构简单。	922 µg·g^-1 CDW	[47]
2020	小立碗藓 Physcomitrella patens	低等植物,萜类背景简单,极高同源重组率。	32.9 µg·g^-1 CDW	[48]
2014	小立碗藓 Physcomitrella patens	低等植物,萜类背景简单,极高同源重组率。	59 µg·g^-1 CDW	[49]
2018	地钱 Marchantia polymorpha	低等植物,基因组小、冗余基因少、易繁殖培养。	1 000.36 μg·g^-1CDW	[50]