枣ABF/AREB基因家族鉴定及其在果实发育中的表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2024-0328

摘要/Abstract

摘要：

【目的】 ABFs（ABRE binding factors）/AREB（ABA responsive element binding）转录因子不仅参与植株的胁迫响应，还影响植株和果实的发育，了解其在枣果实发育过程中的作用，为深入研究枣ABF基因功能提供基础。【方法】 对枣ABF基因进行了全基因组鉴定，并对其理化分析、亚细胞定位、基因结构、基序、系统进化关系、启动子顺式作用元件及表达模式进行了分析。【结果】 在枣基因组共发现5个ABF基因，定位在5条不同假染色体上。ZjABF编码氨基酸序列长度为321-474 aa，等电点为7.75-9.85，亲水系数为-0.807- -0.592，5个成员均为亲水蛋白。不稳定系数为35.62-61.51。基因结构及基序分析表明枣ABF家族成员均存在UTR，分别含有3-5个外显子，6-7个motif。系统发育树将ABF家族分成了3个大类。启动子顺式作用元件分析表明，枣ABF基因家族启动子具有多种响应激素和非生物胁迫相关的顺式作用元件。通过对‘金丝小枣’和‘金魁王’果实发育不同阶段的转录组数据分析，除ZjABF4以外的ZjABFs均有不同的表达，表明枣ABF家族在果实发育过程中发挥了调控作用。利用同源蛋白预测了ZjABF3、ZjABF4和ZjABF5的互作蛋白。【结论】 枣ABF家族基因结构保守，启动子区域存在多个响应激素和非生物胁迫相关的顺式作用元件，在果实发育不同阶段有不同的表达模式。

关键词: 枣, ABF/AREB基因家族, 生物信息学分析, 果实发育

Abstract:

【Objective】 ABFs（ABRE binding factors）/AREB（ABA responsive element binding）transcription factors, which not only participate in the plant stress response, but also affect the development of plants and fruits. Understanding its role in jujube fruit development provides a basis for further study of jujube ABF gene function. 【Method】 We conducted genome-wide identification of jujube ABF gene and analyzed the physicochemical analysis, subcellular localization, gene structure, motifs, phylogenetic relationships, promoter cis-acting elements and expression patterns. 【Result】 Five ABF genes were found in the jujube genome and located on five different pseudochromosomes. The length of the coding amino acid sequence of ZjABF was 321 to 474 aa, the isoelectric point was 7.75 to 9.85, the hydrophilic coefficient was between -0.807 and -0.592, and all five members were hydrophilic proteins. The instability coefficient ranged from 35.62 to 61.51. Gene structure and motif analysis showed that all ABF family members had UTR, containing 3 to 5 exons and 6 to 7 motifs. Phylogenetic trees grouped the ABF family into three large classes. Promoter cis-acting element analysis showed that jujube ABF gene family promoters had multiple cis-acting elements related to response hormones and abiotic stress. By analyzing the transcriptome data at different stages of fruit development, all ZjABFs except ZjABF4 were differently expressed, indicating that the ABF family played a regulatory role in fruit development. Interacting proteins of ZjABF3, ZjABF4, and ZjABF5 were predicted using homologous proteins. 【Conclusion】 The structure of ABF family in jujube is conserved, and there are multiple cis-acting elements in the promoter region that respond to hormones and abiotic stress, with different expression patterns during different stages of fruit development.

Key words: Ziziphus jujuba Mill., ABF/AREB gene family, bioinformatics analysis, fruit development

杨冲, 程莎莎, 艾长丰, 赵璇, 刘孟军. 枣ABF/AREB基因家族鉴定及其在果实发育中的表达分析[J]. 生物技术通报, 2024, 40(11): 184-191.

YANG Chong, CHENG Sha-sha, AI Chang-feng, ZHAO Xuan, LIU Meng-jun. Identification of ABF/AREB Gene Family and Their Expression Analysis in Jujube Fruit[J]. Biotechnology Bulletin, 2024, 40(11): 184-191.

图/表 7

表1 枣ABF的理化性质

Table 1 Physicochemical properties of jujube ABF

基因名称Gene name	基因编号 Gene ID	编码氨基酸长度 Length of encoded amino acids/aa	蛋白质分子量 Molecular weight of protein/kD	等电点 pI	染色体定位 Chromosome location	亲水性GRAVY	不稳定系数 Instability index	脂肪族氨基酸指数 Aliphatic index	亚细胞定位 Subcellular localization
ZjABF1	XP_048337122.1	436	46.24	9.85	Chr.9	-0.614	52.33	66.93	nucl: 14
ZjABF2	XP_060673229.1	470	50.76	7..75	Chr.5	-0.592	35.62	68.09	nucl: 14
ZjABF3	XP_015898748.2	438	48.07	8.47	Chr.11	-0.787	61.51	63.88	nucl: 14
ZjABF4	XP_015872556.3	474	52.64	8.82	Chr.10	-0.798	55.84	64.58	chlo: 6, pero: 5, nucl: 2.5, cyto_nucl: 2
ZjABF5	XP_048326228.1	321	35.58	8.5	Chr.8	-0.807	59.61	66.88	nucl: 14

图1 枣ABF基因家族的基因结构分析

Fig. 1 Analysis of gene structure of ABF gene family in jujube

图2 枣ABF家族蛋白保守结构域分析

Fig. 2 Analysis of conserved motif of ABF family protein in jujube

图3 枣和拟南芥、桃ABF家族的系统进化树分析

Fig. 3 Phylogenetic tree analysis of ABF families from jujube, Arabidopsis and peach（Prunus persica）

图4 枣ABF基因顺式作用元件预测

Fig. 4 Prediction of cis-acting elements of ABF genes in jujube

图5 果实不同发育时期枣ABFs的表达分析 S1：幼果初期；S2：幼果中期；S3：硬核前期；S4：硬核期；S5：白熟期；S6：白熟后期；S7：四分之一着色期；S8：半红期；S9：全红期

Fig. 5 Expression analysis of ABFs at different fruit development stages S1: Early stage of young fruit. S2: Middle stage of young fruit. S3: Pre hard core stage. S4: Hard core stage. S5: White ripening stage. S6: Late stage of white ripening. S7: Quarter coloring stage. S8: Half-red stage. S9: Full-red stage

图6 枣ABFs的互作蛋白预测 A：ZjABF3的互作蛋白预测；B：ZjABF4的互作蛋白预测；C：ZjABF5的互作蛋白预测

Fig. 6 Interacting protein prediction of ABF in jujube A: The interacting protein prediction of ZjABF3. B: The interacting protein prediction of ZjABF4. C: The interacting protein prediction of ZjABF5

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