利用转基因烟草表达人胰高血糖素样肽1 的研究

生物技术通报 ›› 2013, Vol. 0 ›› Issue (1): 139-143.

利用转基因烟草表达人胰高血糖素样肽1 的研究

梁宏伟¹ ,陈发菊¹ ,王玉兵¹ ,李凤兰² ,陆海²

1. 三峡大学生物技术研究中心，宜昌 443002 ； 2. 北京林业大学生物科学与技术学院，北京 100083

收稿日期:2012-07-29 修回日期:2013-01-31 出版日期:2013-01-30 发布日期:2013-01-30
作者简介:梁宏伟，男，讲师，博士，研究方向：植物学、植物细胞工程与分子生物学；E-mail ：lianghwcn@yahoo.com.cn
基金资助:
三峡大学人才科研启动基金项目（KJ2012B023），国家林业局“948”项目(2006-4-73)

Expression of Human Glucagon-like Peptide-1 in Transgenic Tobaccos

Liang Hongwei ¹ Chen Faju ¹ Wang Yubing ¹ Li Fenglan², Lu Hai ²

1. Biotechnology Research Center，China Three Gorges University，Yichang 443002;
2. College of Biological Sciences and Biotechnology，Beijing Forest University，Beijing 100083

Received:2012-07-29 Revised:2013-01-31 Published:2013-01-30 Online:2013-01-30

摘要/Abstract

摘要： 人胰高血糖素样肽1（hGLP1）是一种短肽激素，是近年来备受关注的治疗糖尿病最有前景的候选药物。在设计合成hGLP1 基因并构建植物表达载体的基础上，通过农杆菌介导将hGLP1 基因导入烟草基因组中，获得转化再生植株，经过PCR扩增和Southern blot 分析，证实hGLP1 基因已整合进入6 个株系的烟草基因组中。GUS 组织化学染色表明，与目的基因融合的报告基因在转基因烟草中获得表达。Western blot 检测表明，其中2 个株系转基因烟草叶片中能够检测到hGLP1 融合蛋白的表达。初步的动物试验表明该融合蛋白具有一定的降血糖生物活性。

关键词: 烟草 , 转基因 , 人胰高血糖素样肽1 , 生物活性

Abstract: Human pancreatic glucagon-like peptide-1(hGLP1)is a short peptide hormone with special attention in the treatmentof diabetes, is considered as the most promising candidate medicines. Based on design composition of hGLP1 gene and construction of plantexpression vector, hGLP1 gene was introduced into tobacco through Agrobacterium tumfaciens-mediated method. The PCR amplificationand Southern blot analysis indicated that the hGLP1 gene have been integrated into the genome of six lines of the regenerated tobaccos. GUShistochemistry staining showed that the reporter gene fusion with the target gene was expressed in transgenic tobacco plants. Western blotanalysis confirmed that hGLP1 fusion protein was detected in two lines of transgenic tobacco leaves. The fusion protein demonstrated a certainbiological activity in reducing blood sugar by elementary animal experiments.

Key words: Tobacco , Transgene , Human glucagon-like peptide 1 , Biological activity

梁宏伟, 陈发菊, 王玉兵, 李凤兰, 陆海. 利用转基因烟草表达人胰高血糖素样肽1 的研究[J]. 生物技术通报, 2013, 0(1): 139-143.

Liang Hongwei Chen Faju Wang Yubing Li Fenglan, Lu Hai. Expression of Human Glucagon-like Peptide-1 in Transgenic Tobaccos[J]. Biotechnology Bulletin, 2013, 0(1): 139-143.

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