D-氨基酸检测方法研究进展

生物技术通报 ›› 2013, Vol. 0 ›› Issue (1): 68-72.

D-氨基酸检测方法研究进展

李辉欣, 鞠建松, 张琳琳, 徐书景

河北师范大学生命科学学院，石家庄 050024

收稿日期:2012-08-21 修回日期:2013-01-31 出版日期:2013-01-30 发布日期:2013-01-30
作者简介:李辉欣，女，硕士研究生，研究方向：生物制药；E-mail ：hui_xin126@126.com
基金资助:
河北省自然科学基金项目（C2011205045），河北省科技支撑项目（10205521D），河北省留学回国人员资助项目（20100705），河北省高等学校科学技术研究青年基金项目（2011102），河北师范大学博士启动基金项目（L2009B13）

Research Progress on the Detection Methods of D-amino Acids

Li Huixin, Ju Jiansong, Zhang Linlin, Xu Shujing

College of Life Science，Hebei Normal University，Shijiazhuang 050024

Received:2012-08-21 Revised:2013-01-31 Published:2013-01-30 Online:2013-01-30

摘要/Abstract

摘要： 自然界中存在20 种基本氨基酸，除甘氨酸外都有两种互成镜像的对映体D-型和L-型。长期以来，人们认为D-氨基酸仅存在于微生物中，参与构成细胞壁肽聚糖层。但随着分析方法的发展，人们相继在多种生物体中（包括人体）发现了多种 D-氨基酸，D-氨基酸才引起人们的高度重视，并意识到它们在医药、农药和食品等的组成中起着重要作用。D-氨基酸检测方法还需要不断发展与完善，以便更好地分析和认识D-氨基酸的重要作用。主要介绍酶法、气相层析法、高效液相法及生物传感器等检测方法。

关键词: D-氨基酸 , 检测 , 高效液相 , 生物传感器

Abstract: As we all know there are 20 different kinds of basic amino acids in nature. In addition to glycine, each amino acid has twoenantiomers D-and L-type. D-amino acids have long been considered to be unique to microorganisms, which utilize some D-amino acids toconstruct the peptidoglycan layer of their cell wall. However, several D-amino acids have been detected in many organisms including mammalsbecause of the advance of analytical techniques in the last two decades. It was realized that D-amino acids might play significant roles in livingthings and as the important component of medicine, pesticide and food. In order to get a better analyzing and understanding the important role ofD-amino acids, the analytical methods for D-amino acids detection are constantly developing. Herein, several methods include enzymatic method,Gas chromatography, HPLC and biosensor assay were introduced.

Key words: D-amino acid , Detection , HPLC , Biosensor

李辉欣, 鞠建松, 张琳琳, 徐书景. D-氨基酸检测方法研究进展[J]. 生物技术通报, 2013, 0(1): 68-72.

Li Huixin, Ju Jiansong, Zhang Linlin, Xu Shujing. Research Progress on the Detection Methods of D-amino Acids[J]. Biotechnology Bulletin, 2013, 0(1): 68-72.

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