巴西橡胶树HbNTL1 基因启动子的克隆与序列分析

生物技术通报 ›› 2013, Vol. 0 ›› Issue (1): 83-88.

巴西橡胶树HbNTL1 基因启动子的克隆与序列分析

康桂娟 ,黎瑜,曾日中,聂智毅

中国热带农业科学院橡胶研究所农业部橡胶树生物学与遗传资源利用重点实验室和省部共建国家重点实验室培育基地—海南省热带作物栽培生理学重点实验室，儋州 571737

收稿日期:2012-06-14 修回日期:2013-01-31 出版日期:2013-01-30 发布日期:2013-01-30
作者简介:康桂娟，女，博士，助理研究员，研究方向：橡胶树分子生物学；E-mail ：guijuan.kang@gmail.com
基金资助:
中国热带农业科学院橡胶研究所基本科研业务费专项资金（1630022011024）

Cloning and Sequence Analysis of HbNTL1 Promoter from Hevea brasiliensis

Kang Guijuan, Li Yu ,Zeng Rizhong ,Nie Zhiyi

Key Laboratory of Ministry of Agriculture for Rubber Biology and Genetic Resource Utilization， State Key Laboratory Breeding Base of Cultivation ＆ Physiology for Tropical Crops，Rubber Research Institute，Chinese Academy of Tropical Agricultural Sciences（CATAS），Danzhou 571737

Received:2012-06-14 Revised:2013-01-31 Published:2013-01-30 Online:2013-01-30

摘要/Abstract

摘要： 膜结合NAC 转录因子（NTLs）是植物NAC 转录因子家族中一类C 端具有跨膜结构域（transmembrane motifs，TMs）的转录调控因子，在植物生长发育、激素调节和逆境胁迫应答中具有重要的功能。根据巴西橡胶树（Hevea brasiliensis）膜结合类NAC 转录因子HbNTL1 基因cDNA 序列，利用基因组步移的方法从巴西橡胶树叶片基因组DNA 中克隆获得了HbNTL1 基因上游1 718 bp 的调控片段。序列分析表明，该段序列含有一个典型的真核生物核心启动子区域，转录起始位点A 位于起始密码子上游206 bp 处。该启动子序列除了含有多个TATA-box、CAAT-box 等基本顺式作用元件外，还存在赤霉素、茉莉酸和脱落酸等激素响应元件以及大量逆境胁迫诱导相关的顺式调控元件，如ABRE、DOFCOREZM、MYBCORE、W-box 和MYCCONSENSUSATHSE等反应元件，表明HbNTL1 转录因子可能是一个逆境胁迫相关NAC 转录因子，在橡胶树抵御逆境胁迫的生理过程中具有重要功能。

关键词: 巴西橡胶树 , HbNTL1 , 启动子 , 顺式调控元件 , 逆境胁迫

Abstract: Membrane-bound NAC transcription factors(NTLs)are a group of NAC transcription factors with transmembrane motif(TMs)in the C-terminal region, play important roles in plant growth and development, hormone regulations and stress responses. A 1 718 bp5' regulatory sequence of HbNTL1 was cloned from leaf genomic DNA of H. brasiliensis based on the cDNA sequences using Genome Walkingmethod. Sequence analysis showed that it consisted of a typical core promoter region of eukaryotic, and the transcription start site(TSS, A)located 206 bp upstream of the initiation codon ATG. The promoter contains the basic elements ：TATA-box, CAAT-box, and some hormoneresponsive elements as well as and multiple stress-induced elements, such as, ABRE, DOFCOREZM, MYBCORE, MYCCONSENSUSATHSEand W-box. It is suggested that HbNTL1 gene may play important roles in response to various stresses in rubber tree as a stress-related NACtranscription factor.

Key words: Hevea brasiliensis , HbNTL1 promoter , cis-acting element , Stress responsiveness

康桂娟, 黎瑜, 曾日中, 聂智毅. 巴西橡胶树HbNTL1 基因启动子的克隆与序列分析[J]. 生物技术通报, 2013, 0(1): 83-88.

Kang Guijuan, Li Yu, Zeng Rizhong, Nie Zhiyi. Cloning and Sequence Analysis of HbNTL1 Promoter from Hevea brasiliensis[J]. Biotechnology Bulletin, 2013, 0(1): 83-88.

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