长距离实时定量PCR技术及其在DNA损伤研究中的应用

生物技术通报 ›› 2013, Vol. 0 ›› Issue (5): 52-57.

长距离实时定量PCR技术及其在DNA损伤研究中的应用

梁姣¹, 王淑红¹, 张子平², 王艺磊¹

(1.集美大学水产学院，厦门 361021；2.西东大学生物系，新泽西州 07079)

收稿日期:2012-12-12 修回日期:2013-05-24 出版日期:2013-05-24 发布日期:2013-05-24
作者简介:梁姣，女，硕士研究生，研究方向：水产生物技术；E-mail：18259226570@163.com
基金资助:
集美大学创新团队基金项目(2010A001)

Long Amplicon Real-time Quantitative PCR Technology and Its Application in DNA Damage Detection

Liang Jiao¹, Wang Shuhong¹, Zhang Ziping², Wang Yilei¹

(1. Fisheries College，Jimei University，Xiamen 361021；2. Department of Biological Science，Seton Hall University，New Jersey 07079)

Received:2012-12-12 Revised:2013-05-24 Published:2013-05-24 Online:2013-05-24
About author:王淑红，女，博士，副教授；E-mail：shwang@jmu.edu.cn

摘要/Abstract

摘要： DNA损伤作为评价环境化合物的遗传毒性、生物个体环境暴露水平和肿瘤罹患危险度的指标越来越受到人们的关注。检测DNA损伤的方法很多，而PCR技术由于所需样品量少，检测灵敏度高等优点，近年来发展很快。综述PCR技术在DNA损伤研究中的应用，并重点介绍新近发展的长距离实时定量PCR(long amplicon real-time quantitative PCR，LA-real time QPCR)的关键技术。

关键词: 长距离实时定量PCR, DNA损伤, 环境化合物

Abstract: DNA damage as an endpoint for evaluating genotoxicity of environmental compounds and organism levels of environmental exposure as well as the risk of tumor is always the research focus. Several methods were developed for detecting DNA damage, PCR technology, due to its little DNA needs and high accuracy is widely used in DNA damage detection recently. Here, we review the application of PCR on DNA damage detection, more over, the technical key steps of a newly developed technology called the long amplicon real-time quantitative PCR (LA-real time QPCR) also were detailed .

Key words: Long amplicon real-time quantitative, PCR DNA damage, Environmental compounds

梁姣, 王淑红, 张子平, 王艺磊. 长距离实时定量PCR技术及其在DNA损伤研究中的应用[J]. 生物技术通报, 2013, 0(5): 52-57.

Liang Jiao, Wang Shuhong, Zhang Ziping, Wang Yilei. Long Amplicon Real-time Quantitative PCR Technology and Its Application in DNA Damage Detection[J]. Biotechnology Bulletin, 2013, 0(5): 52-57.

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