生物技术通报 ›› 2013, Vol. 0 ›› Issue (6): 155-159.

• 研究报告 • 上一篇    下一篇

微生物菌体对Cd2+等重金属离子的吸附研究

周广麒1, 任铮宇1, 杨洪泽2, 李荣娇2, 邢艳杰2, 赵玉清2   

  1. (1.大连工业大学生物工程学院, 大连 116034;2.大连民族学院生命科学学院, 大连 116605)
  • 收稿日期:2013-06-20 修回日期:2013-06-20 出版日期:2013-06-20 发布日期:2013-06-20
  • 作者简介:周广麒, 男, 教授, 研究方向: 微生物技术及产业化;E-mail: dlzgq@126. com

Bacterial Adsorption of Heavy Metal Ions such as Cd2+

Zhou Guangqi1 Ren Zhengyu1 Yang Hongze2 Li Rongjiao2 Xing Yanjie2 Zhao Yuqing2   

  1. (1. School of Biological Engineering, Dalian Polytechnic University, Dalian 116034;2. College of Life Science, Dalian Nationalities University, Dalian 116605)
  • Received:2013-06-20 Revised:2013-06-20 Published:2013-06-20 Online:2013-06-20

摘要: 旨在研究筛选的微生物菌体对溶液中Cd2+等重金属离子的吸附及解吸的方法。结果表明, 微生物菌体对Cd2+ 的适宜吸附时间为60 min。通过红外光谱分析, 明确了菌体表面某些多糖和蛋白质中的酰胺基、羟基、羰基等官能团在吸附过程中的重要作用。使用0.1 mol/L NH4OH溶液对微生物菌体进行预处理, 能大大提高其对Cd2+、Ni2+和Pb2+的吸附能力;0.02 mol/L HCl溶液对各重金属离子的解吸率都在96%以上, 解吸后的菌体可以重复用于吸附。

关键词: 微生物菌体, 重金属离子, 红外光谱, 吸附, 解吸

Abstract: This paper studies the bacterial adsorption and desorption of cadmium ions and other heavy metal ions. The test results showed that the appropriate adsorption time of cadmium ions by the bacteria is 60 min. Infrared spectral analysis indicates that the amide, hydroxyl and carbonyl functional groups of polysaccharides and proteins on cell surface play a key role in the adsorption process. Further experimental results show that pretreatment of 0.1 mol/L NH4OH solution on cell can significantly increase the bacterial adsorption capacity for Cd2+, Ni2+ and Pb2+;0.02 mol/L HCl solution can make more than 96% of the heavy metal ions to be desorbed from the microbial cell, and after desorption, the bacteria can be reused.

Key words: Microbial bacteria, Heavy metal ions Infrared, Spectroscopy, Adsorption, Desorption