表面活性剂对纤维素酶解的影响及机理

doi:10.13560/j.cnki.biotech.bull.1985.2019-0158

摘要/Abstract

摘要： 随着能源危机和环境问题的日益紧张,寻求一种环境友好、可持续和充足的替代能源变得越来越重要。以木质纤维素生物质为原料,发酵生产生物燃料和其他生物材料的研究得到了越来越广泛的关注,而生物酶法高效降解木质纤维素是生物质转化利用的研究热点。添加表面活性剂（SF）可显著提高纤维素的酶解效率和转化率,但仍受到诸多因素的影响。综述了表面活性剂提升纤维素酶水解能力的研究现状,归纳了其种类与浓度、纤维素酶的来源与组成以及底物结构和水解条件对表面活性剂作用效果产生的影响,并从其对酶的吸附性质、酶的稳定性与活性、底物表面结构的影响等方面分析了表面活性剂提高纤维素的酶水解率和转化率的作用机制。并针对目前的研究现状,对今后应重点研究的方向进行了展望,即深入探究SF、纤维素酶与底物三者之间的分子作用力在稳定纤维素酶活性、改变酶吸附性质以及底物表面性质等方面发挥的作用,旨在为木质纤维素生物质的酶解转化研究提供理论借鉴。

关键词: 表面活性剂, 木质纤维素, 水解, 纤维素酶, 稳定性, 吸附

Abstract: With the increasing tension of energy crisis and environmental problems,it is becoming growingly important to seek an environmentally friendly,sustainable and adequate energy alternative. The research on fermentation of lignocellulose biomass to produce biofuels and other biomaterials has attracted more and more attention. Enzymatic degradation of lignocellulose is a hot topic in biomass conversion and utilization. Surfactant（SF）can significantly improve the enzymatic hydrolysis efficiency and conversion of cellulose,but it is still affected by many factors. Here we reviewed the research status of SF on enhancing the hydrolysis ability of cellulase,and summarized the types and concentration of SF,the source and composition of cellulase,the influence of substrate structure,and hydrolysis conditions on the effectiveness of SF. Further,we analyzed the mechanism of SF enhancing cellulosic enzyme efficiency and conversion rate based on the adsorption properties of enzymes,the stability and activity of enzyme,and the surface structure of substrate. In view of the current research situation,we prospected the future research directions,that is,further exploring the role of molecular interaction among SF,cellulase and substrates in stabilizing cellulase activity,changing the adsorption properties of enzymes,and the surface properties of substrates,aiming to provide a theoretical reference for the enzymatic transformation of lignocellulose biomass.

Key words: surfactant, lignocellulose, hydrolysis, cellulase, stability, adsorption

张家顺, 高丽莉, 马江山, 刘高强. 表面活性剂对纤维素酶解的影响及机理[J]. 生物技术通报, 2019, 35(9): 11-20.

ZHANG Jia-shun, GAO Li-li, MA Jiang-shan, LIU Gao-qiang. Effect of Surfactant on Cellulase Hydrolysis and Its Mechanism[J]. Biotechnology Bulletin, 2019, 35(9): 11-20.

参考文献

[1] Himmel ME, Ding SY, Johnson DK, et al.Biomass recalcitrance:engineering plants and enzymes for biofuels production[J]. Science, 2007, 315(5813):804-807.
[2] Sindhu R, Binod P, Panden A, et al.Biological pretreatment of lignocellulosic biomass-an overview[J]. Bioresource Technology, 2016, 199:76-82.
[3] Rocha-Martin J, Martinez-Bernal C, Perez-Cobas Y, et al.Additives enhancing enzymatic hydrolysis of lignocellulosic biomass[J]. Bioresource Technology, 2017, 244(Pt 1):48-56.
[4] Chakraborty R, Chatterjee S, Mukhopadhyay P, et al.Progresses in waste biomass derived catalyst for production of biodiesel and bioethanol:a review[J]. Procedia Environmental Sciences, 2016, 35:46-554.
[5] Xing Y, Bu L, Sun D, et al.Enhancement of high-solids enzymatic hydrolysis and fermentation of furfural residues by addition of Gleditsia saponin[J]. Fuel, 2016, 177:142-147.
[6] Pennacchio A, Ventorino V, Cimini D.Isolation of new cellulase and xylanase producing strains and application to lignocellulosic biomasses hydrolysis and succinic acid production[J]. Bioresource Technology, 2018, 259:325-333.
[7] Makhuvele R, Ncube I, Rensburg ELJ, et al.Isolation of fungi from dung of wild herbivores for application in bioethanol production[J]. Brazilian Journal of Microbiology, 2017, 48(4):648-655.
[8] Kuhad RC, Deswal D, Sharma S, et al.Revisiting cellulase production and redefining current strategies based on major challenges[J]. Renewable and Sustainable Energy Reviews, 2016, 55:249-272.
[9] Karimi K, Taherzadeh MJ.A critical review on analysis in pretreatment of lignocelluloses:Degree of polymerization, adsorption/desorption, and accessibility[J]. Bioresource Technology, 2016, 203:348-356.
[10] Kristensen JB, Borjesson J, Bruun MH, et al.Use of surface active additives in enzymatic hydrolysis of wheat straw lignocellulose[J]. Enzyme and Microbial Technology, 2007, 40(4):888-895.
[11] Eriksson T, Borjesson J, Tjerneld F.Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose[J]. Enzyme and Microbial Technology, 2002, 31(3):353-364.
[12] Cai C, Qiu X, Lin X, et al.Improving enzymatic hydrolysis of lignocellulosic substrates with pre-hydrolysates by adding cetyltrimethylammonium bromide to neutralize lignosulfonate[J]. Bioresource Technology, 2016, 216:968-975.
[13] Lin XL, Zhou MS, Wang SY, et al.Synthesis, structure, and dispersion property of a novel lignin-based polyoxyethylene ether from kraft lignin and poly(ethylene glycol)[J]. Acs Sustainable Chemistry and Engineering, 2014, 2(7):1902-1909.
[14] Zajjc JE, Panchal CJ.Bio-emulsifiers[J]. Crc Critical Reviews in Microbiology, 1976, 5(1):39-66.
[15] Talukder MMR, Hai YG, Puah SM.Interaction of silica with cellulase and minimization of its inhibitory effect on cellulose hydrolysis[J]. Biochemical Engineering Journal, 2017, 18:91-96.
[16] Kamsani N, Salleh MM, Basriet SA, et al.Effects of surfactant on the enzymatic degradation of oil palm empty fruit bunch(OPEFB)[J]. Waste and Biomass Valorization, 2017(3):1-8.
[17] Parnthong J, Kungsanant S, Chavadej S.Enhancement of enzymatic hydrolysis of lignocellulosic materials by nonionic surfactant[J]. Key Engineering Materials, 2017, 757:151-155.
[18] Kim HJ, Kim SB, Kim CJ.The effects of nonionic surfactants on the pretreatment and enzymatic hydrolysis of recycled newspaper[J]. Biotechnology and Bioprocess Engineering, 2007, 12(2):147-151.
[19] Li C, Zhong L, Lan FH, et al.Effect of cellobiase and surfactant supplementation on the enzymatic hydrolysis of pretreated wheat straw[J]. Bioresources, 2011, 6(4):3850-3858.
[20] Parnthong J, Kungsanant S, Chavadej S.The Influence of nonionic surfactant adsorption on enzymatic hydrolysis of oil palm fruit bunch[J]. Applied Biochemistry and Biotechnology, 2018(1):1-14.
[21] Cai C, Qiu XQ, Zeng MJ, et al.Using polyvinylpyrrolidone to enhance the enzymatic hydrolysis of lignocelluloses by reducing the cellulase non-productive adsorption on lignin[J]. Bioresource Technology, 2016, 227:74-81.
[22] Sipos B, Szilagyi M, Sebestyen Z, et al.Mechanism of the positive effect of poly(ethylene glycol)addition in enzymatic hydrolysis of steam pretreated lignocelluloses[J]. Comptes Rendus Biologies, 2011, 334(11):812-823.
[23] Li XL, Li M, Pu YQ, et al.Inhibitory effects of lignin on enzymatic hydrolysis:The role of lignin chemistry and molecular weight[J]. Renewable Energy, 2018, 123:664-674.
[24] Mizutani C, Sethumadhavan K, Howley P, et al.Effect of a nonionic surfactant on trichoderma cellulase treatments of regenerated cellulose and cotton yarns[J]. Cellulose, 2002, 9(1):83-89.
[25] Gupta R, Lee YY.Mechanism of cellulase reaction on pure cellulosic substrates[J]. Biotechnology and Bioengineering, 2010, 102(6):1570-1581.
[26] Lou HM, Zhu JY, Lan TQ, et al.pH-Induced lignin surface modification to reduce nonspecific cellulase binding and enhance enzymatic saccharification of lignocelluloses[J]. Chemsuschem, 2013, 6(5):919-927.
[27] Wang ZJ, Lan TQ, Zhu JY.Lignosulfonate and elevated pH can enhance enzymatic saccharification of lignocelluloses[J]. Biotechnology for Biofuels, 2013, 6(1):9.
[28] Yang MH, Zhang AM, Liu BB, et al.Improvement of cellulose conversion caused by the protection of Tween-80 on the adsorbed cellulase[J]. Biochemical Engineering Journal, 2011, 56(3):125-129.
[29] Okino S, Ikeo M, Makoto U, et al.Effects of Tween 80 on cellulase stability under agitated conditions[J]. Bioresource Technology, 2013, 142(8):35-539.
[30] Jorgenson H, Vibr-Pedersen J, Larsen J, et al.Liquefaction of lignocellulose at high-solids concentrations[J]. Biotechnology and Bioengineering, 2010, 96(5):862-870.
[31] Ma XX, Yue GJ, Yu JL, et al.Enzymatic hydrolysis of cassava bagasse with high solid loading[J]. Journal of Biobased Materials and Bioenergy, 2011, 5(2):275-281.
[32] Reese ET.Inactivation of cellulase by shaking and its prevention by surfactants[J]. J Appl Biochem, 1980, 2(1):36-39.
[33] Ouyang J, Dong ZW, Song XY, et al.Improved enzymatic hydrolysis of microcrystalline cellulose(Avicel PH101)by polyethylene glycol addition[J]. Bioresource Technology, 2010, 101(17):6685-6691.
[34] Borjesson J, Engqvist M, Sipos B, et al.Effect of poly(ethylene glycol)on enzymatic hydrolysis and adsorption of cellulase enzymes to pretreated lignocellulose[J]. Enzyme and Microbial Technology, 2007, 41(1):186-195.
[35] Cai C, Pang YX, Zhan XJ, et al.Using temperature-responsive zwitterionic surfactant to enhance the enzymatic hydrolysis of lignocelluloses and recover cellulase by cooling[J]. Bioresource Technology, 2017, 243:1141-1148.
[36] Alhammad A, Adewale P, Kuttiraja M, et al.Enhancing enzyme-aided production of fermentable sugars from poplar pulp in the presence of non-ionic surfactants[J]. Bioprocess and Biosystems Engineering, 2018. 41(8):1-10.
[37] Zhou Y, Chen HM, Feng Q, et al.Non-ionic surfactants do not consistently improve the enzymatic hydrolysis of pure cellulose[J]. Bioresource Technology, 2015, 182:136-143.
[38] Liu J, Shi JG, Jian L, et al.Characterization of the interaction between surfactants and enzymes by fluorescence probe[J]. Enzyme and Microbial Technology, 2011, 49(4):360-365.
[39] Chandel AK, Silva SSD.Sustainable degradation of lignocellulosic biomass - techniques, applications and commercialization[J]. Bioresource Engineering, 2013, 275:61-64.
[40] Jiang F, Qian C, Esker AR, et al.Effect of non-ionic surfactants on dispersion and polar interactions in the adsorption of cellulases onto lignin[J]. Journal of Physical Chemistry B, 2017, 121(41):9607-9620.
[41] Cai C, Zhan XJ, Zeng MJ, et al.Using recyclable pH-responsive lignin amphoteric surfactant to enhance the enzymatic hydrolysis of lignocelluloses[J]. Green Chemistry, 2017. 19(22):5479-5487.
[42] Lin XL, Qiu XQ, Yuan L, et al.Lignin-based polyoxyethylene ether enhanced enzymatic hydrolysis of lignocelluloses by dispersing cellulase aggregates[J]. Bioresource Technology, 2015, 185:165-170.
[43] Lin XL, Qiu XQ, Lou HM, et al.Enhancement of lignosulfonate-based polyoxyethylene ether on enzymatic hydrolysis of lignocelluloses[J]. Industrial Crops & Products, 2016. 80:86-92.
[44] Li JH, Li SZ, Fan CY, et al.The mechanism of poly(ethylene glycol)4000 effect on enzymatic hydrolysis of lignocellulose[J]. Colloids and Surfaces Biointerfaces, 2012, 89(1):203-210.
[45] Lin XL, Cai C, Huang JH, et al.Understanding the effect of the complex of lignosulfonate and cetyltrimethylammonium bromide on the enzymatic digestibility of cellulose[J]. Energy and Fuels, 2017, 31(1):672-678.
[46] Xin DL, Yang M, Chen X, et al.Improving the hydrolytic action of cellulases by Tween 80:Offsetting the lost activity of cellobiohydrolase Cel7A[J]. Acs Sustainable Chemistry and Engineering, 2017, 5(12):11339-11345.
[47] Lou HM, Zeng MJ, Hu QY, et al.Nonionic surfactants enhanced enzymatic hydrolysis of cellulose by reducing cellulase deactivation caused by shear force and air-liquid interface[J]. Bioresource Technology, 2017, 249:1-8.
[48] Bhagia S, Dhir R, Kumar R, et al.Deactivation of cellulase at the air-liquid interface is the main cause of incomplete cellulose conversion at low enzyme loadings[J]. Scientific Reports, 2018. 8(1):1350.
[49] Zhang M, Ouyang J, Liu BT, et al.Comparison of hydrolysis efficiency and enzyme adsorption of three;Different cellulosic materials in the presence of poly(ethylene Glycol)[J]. Bioenergy Research, 2013, 6(4):1252-1259.
[50] Zheng J, Li LY, Chen SF, et al.Molecular simulation study of water interactions with oligo(ethylene glycol)-terminated alkanethiol self-assembled monolayers[J]. Langmuir the Acs Journal of Surfaces and Colloids, 2004, 20(20):8931-8938.
[51] Kaar WE, Holtzapple MT.Benefits from Tween during enzymic hydrolysis of corn stover[J]. Biotechnology and Bioengineering, 2015, 59(4):419-427.
[52] Seo DJ, Fujita H, Sakoda A.Structural changes of lignocelluloses by a nonionic surfactant, Tween 20, and their effects on cellulase adsorption and saccharification[J]. Bioresource Technology, 2011. 102(20):9605-9612.
[53] Kim W, Gamo Y, Sani YM, et al.Effect of Tween 80 on hydrolytic activity and substrate accessibility of carbohydrolase I(CBH I)from Trichoderma viride[J]. Asian Australasian Journal of Animal Sciences, 2006, 19(5):684-689.
[54] Helle SS, Duff S JB, Cooper DG.Effect of surfactants on cellulose hydrolysis[J]. Biotechnology and Bioengineering, 2010, 42(5):611-617.
[55] Lin XL, Qiu XQ, Zhu DM, et al.Effect of the molecular structure of lignin-based polyoxyethylene ether on enzymatic hydrolysis efficiency and kinetics of lignocelluloses[J]. Bioresource Technology, 2015, 193:266-273.
[56] Hemmatinejad N, Vahabzadeh F, Kordestani SS.Effect of surfactants on enzymatic hydrolysis of cellulosic fabric[J]. Iranian Polymer Journal, 2002, 11(5):333-338.