新疆陆地棉组成型表达海岛棉脂质转移酶基因GbLTP1和GbLTP3特性研究

生物技术通报 ›› 2013, Vol. 0 ›› Issue (6): 87-93.

新疆陆地棉组成型表达海岛棉脂质转移酶基因GbLTP1和GbLTP3特性研究

沈海涛^{1, 2}, 王爱英^{1, 2}, 李予霞¹, 祝建波^{1, 2}

(1.石河子大学生命科学学院, 石河子 832003;2.石河子大学农业生物技术重点实验室, 石河子 832003)

收稿日期:2013-06-20 修回日期:2013-06-20 出版日期:2013-06-20 发布日期:2013-06-20
作者简介:沈海涛, 男, 硕士, 助理研究员, 研究方向: 植物基因工程;E-mail: ghost521@126.com
基金资助:
国家转基因专项(2011zx08011-002), 兵团青年科技创新资金专项(2013cb010)

Study on Characteristics of Constitutive Expression of GbLTP1 and GbLTP3 Genes in Land-cotton

Shen Haitao^{1, 2} Wang Aiying^{1, 2} Li Yuxia¹ Zhu Jianbo^{1, 2}

(1. College of Life Sciences of Shihezi University, Shihezi 832003;2. Key Laboratory of Agriculture Biotechnology of Shihezi University, Shihezi 832003)

Received:2013-06-20 Revised:2013-06-20 Published:2013-06-20 Online:2013-06-20

摘要/Abstract

摘要： 根据LTPs基因保守序列从海岛棉品种新海21中克隆两个脂质转移酶基因GbLTP1和GbLTP3。构建组成型表达载体pCAMBIA2301-GbLTP1和pCAMBIA2301-GbLTP3两个植物表达载体转化陆地棉。通过卡那霉素筛选、PCR和RT-PCR检测, 获得转GbLTP1基因棉花和转GbLTP3基因棉花各两个株系。利用SPSS18.0分析转基因后代抗病性、农艺性状和经济性状影响。结果显示, 通过将GbLTP3与GbLTP1基因导入新疆陆地棉, 棉花抗黄、枯萎病抗性和纤维品质相对于受体显著提高, 特别是纤维长度和整齐度达到极显著水平(P<0.05) 。外源基因GbLTP3与GbLTP1基因导入不会影响棉花农艺性状、产量性状。

关键词: 脂质转移酶, 转基因棉花, 棉花枯萎病和黄萎病, 农艺性状, 纤维品质

Abstract: According to the conserved sequences of LTPs gene from G. barbadense variety Xinhai 21 clone two lipid transferase gene GbLTP1 and GbLTP3. Constructing the constitutive expression vector pCAMBIA2301-GbLTP1 and pCAMBIA2301-GbLTP1 two plant expression vectors were transformed into upland cotton. By kanamycin screening, PCR and RT-PCR technique, to obtain transgenic GbLTP1 cotton and transgenic GbLTP3 cotton in all two strains. The progeny of transgenic plants disease resistance, agronomic and economic traits analysis, results showed that: the expression of GbLTP3 and GbLTP1 gene and Xinjiang upland cotton resistance to Fusarium wilt, resistance to yellow, and no significant increase of cotton. Effects of exogenous GbLTP3 gene and GbLTP1 gene into not agronomic traits, yield traits of cotton fiber quality, especially on fiber length has a certain improving effect.

Key words: Lipid transfer protein, Transgenic cotton, Cotton fusarium wilt and verticillium wilt, Agronomic characters, Fiber quality

沈海涛, 王爱英, 李予霞, 祝建波. 新疆陆地棉组成型表达海岛棉脂质转移酶基因GbLTP1和GbLTP3特性研究[J]. 生物技术通报, 2013, 0(6): 87-93.

Shen Haitao, Wang Aiying, Li Yuxia, Zhu Jianbo. Study on Characteristics of Constitutive Expression of GbLTP1 and GbLTP3 Genes in Land-cotton[J]. Biotechnology Bulletin, 2013, 0(6): 87-93.

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