DNA条形码鉴别内蒙古地区啮齿动物

生物技术通报 ›› 2013, Vol. 0 ›› Issue (8): 94-98.

DNA条形码鉴别内蒙古地区啮齿动物

常子丽, 刘芳, 王建军, 李建云, 胡艳红, 武正华, 王浩辉, 李科, 杨志羡, 马志东, 范蒙光, 张忠兵

（内蒙古自治区地方病防治研究中心，呼和浩特 010031）

收稿日期:2013-03-04 修回日期:2013-08-11 出版日期:2013-08-11 发布日期:2013-09-02
作者简介:常子丽，女，博士，研究方向：鼠疫预防控制；E-mail：changzili_cn@163.com
基金资助:
2012年卫生公益性行业科研专项（201202021）

Identification of Rodents by DNA Barcoding in Inner Mongolia Area

Chang Zili, Liu Fang, Wang Jianjun, Li Jianyun, Hu Yanhong, Wu Zhenghua, Wang Haohui, Li Ke, Yang Zhixian, Ma Zhidong, Fan Mengguang, Zhang Zhongbing

（Inner Mongolia Center of Endemic Disease Control and Research，Hohhot 010031）

Received:2013-03-04 Revised:2013-08-11 Published:2013-08-11 Online:2013-09-02

摘要/Abstract

摘要： 为探究应用DNA条形码技术对啮齿动物进行分子生物学鉴定的可行性，检测了内蒙古地区7种啮齿动物的59条线粒体COI基因部分序列，用MEGA5.0软件计算GC含量、遗传距离，采用邻接法（NJ）构建分析系统发育树。结果发现，所有序列富含AT，大部分鼠种都有特定的氨基酸组分。种内平均遗传距离为0.6%，不同鼠种间平均遗传距离为23.1%，NJ进化树能清楚的区分7个不同的类群。所以认为通过COI基因应用DNA条码技术可以鉴别内蒙古地区的啮齿动物。

关键词: DNA条形码, 啮齿动物, CO, I基因

Abstract: To study the feasibility of DNA barcoding in the identification of rodents. 59 specimens of 7 species were sequenced（barcoded）for a 657 bp region of the mitochondrial cytochrome oxidase subunit I gene（COI）in Inner Mongolia Area, China. The GC content and genetic distance was calculated using MEGA5.0, and the phylogenetic trees were constructed with Neighbor-Joining（NJ）method. The results showed that AT contents was larger in all sequences, mostly species had specified amino acid component. The average intraspecific genetic distance（K2P）was 0.6% and the average interspecific genetic distance was 23.1%. A Neighbor-Joining tree showed 7 major clusters apparent. We conclude that COI sequencing of DNA barcoding can be used to identify rodents species in Inner Mongolia.

Key words: DNA barcoding, Rodent, COI gene

常子丽, 刘芳, 王建军, 李建云, 胡艳红, 武正华, 王浩辉, 李科, 杨志羡, 马志东, 范蒙光, 张忠兵. DNA条形码鉴别内蒙古地区啮齿动物[J]. 生物技术通报, 2013, 0(8): 94-98.

Chang Zili, Liu Fang, Wang Jianjun, Li Jianyun, Hu Yanhong, Wu Zhenghua, Wang Haohui, Li Ke, Yang Zhixian, Ma Zhidong, Fan Mengguang, Zhang Zhongbing . Identification of Rodents by DNA Barcoding in Inner Mongolia Area[J]. Biotechnology Bulletin, 2013, 0(8): 94-98.

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